A5001193542- Multiple Myeloma Research and Treatments
- Bone Metabolism and Diseases
- Chemokine receptors and signaling
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- interferon and immune responses
- Bone health and osteoporosis research
- Bone health and treatments
- Circular RNAs in diseases
- Immune cells in cancer
- MicroRNA in disease regulation
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Circadian rhythm and melatonin
- RNA Research and Splicing
- Hedgehog Signaling Pathway Studies
- Pancreatitis Pathology and Treatment
- Cardiac Ischemia and Reperfusion
- Telomeres, Telomerase, and Senescence
- NF-κB Signaling Pathways
Sichuan University
2006-2025
Shandong Tumor Hospital
2024-2025
Shandong First Medical University
2021-2025
Tianjin Medical University
2018-2024
West China Hospital of Sichuan University
2021-2023
Guiyang Medical University
2020-2023
Tianjin Medical University General Hospital
2023
Southwest Medical University
2021
Central Clinical Hospital
2021
Yanbian University Hospital
2021
Abstract Excessive monocyte activation with the development of excessive or uncontrolled release proinflammatory cytokines often results in host tissue injury and even death patients pneumonia caused by 2019 novel coronavirus. However, changes cytokine profiles coronavirus disease (COVID-19) patients, as well underlying mechanisms that are involved, remain unknown. Using a array containing 174 inflammation-related cytokines, we found significantly altered severe COVID-19 compared those mild...
Abstract Senescence of bone marrow mesenchymal stem cells (BMSCs) impairs stemness and osteogenic differentiation, but the key regulators for senescence related osteogenesis are not well defined. Herein, we screened gene expression profiles human BMSCs from young old donors identified that elevation nucleosome assembly protein 1‐like 2 (NAP1L2) was correlated with BMSC impaired osteogenesis. Elevated NAP1L2 observed in replicative cell induced vitro, age‐related senescent mouse vivo,...
Development of chemoresistance is the main reason for failure clinical management multiple myeloma (MM), but genetic and epigenetic aberrations that interact to confer such remains unknown. In present study, we find high steroid receptor coactivator-3 (SRC-3) expression correlated with relapse/refractory poor outcomes in MM patients treated bortezomib (BTZ)-based regimens. Furthermore, immortalized cell lines, SRC-3 enhances resistance proteasome inhibitor (PI)-induced apoptosis....
Abstract Background Aging‐associated osteoporosis is frequently seen in the elderly clinic, but efficient managements are limited because of unclear nosogenesis. The current study aims to investigate role melatonin on senescent bone marrow stromal cells (BMSCs) and underlying regulating mechanism. Methods Melatonin levels were tested by ELISA. Gene expression profiles performed RNA‐sequencing, enrichment H3K36me2 gene promoters was analyzed Chromatin Immunoprecipitation Sequencing...
Background Immunotherapies targeting CD38 have demonstrated salient efficacy in relapsed/refractory multiple myeloma (MM). However, loss of antigen and outgrowth negative plasma cells emerged as a major obstacle clinics. All-trans retinoic acid (ATRA) has been reported to upregulate expression, but the mechanism adaptive genetic background remain unexplored. Methods The ATRA upregulating expression MM is evaluated by flow cytometry. interaction between NSD2 RARα analyzed immunoprecipitation,...
We examined the effects of tumor necrosis factor-α (TNFα) and interleukin-6 (IL6) gene knockout in preserving bone loss induced by ovariectomy (OVX) mechanisms involved metabolism. Twenty female wild-type (WT), TNFα-knockout (TNFα-/-) or IL6-knockout (IL6-/-) mice aged 12 weeks were sham-operated (SHAM) subjected to OVX killed after 4 weeks. Bone mass skeletal microarchitecture determined using micro-CT. marrow stromal cells (BMSCs) from all three groups (WT, TNFα-/- IL6-/-) differentiate...
Background Accumulating evidence indicates that high-fat diet (HFD) is a controllable risk factor for osteoporosis, but the underlying mechanism remains to be elucidated. As primary biological barrier nutrient entry into human body, composition and function of gut microbiota (GM) can altered rapidly by HFD, which may trigger abnormal bone metabolism. In current study, we analyzed signatures GM serum metabolomics in HFD-induced loss explored potential correlations metabolites on HFD-related...
The chromosomal t(4;14) (p16;q32) translocation drives high expression of histone methyltransferase nuclear SET domain-containing 2 (NSD2) and plays vital roles in multiple myeloma (MM) evolution progression. However, the mechanisms NSD2-driven epigenomic alterations chemoresistance to proteasome inhibitors (PIs) are not fully understood. Using a CRISPR/Cas9 sgRNA library bone marrow-bearing MM model, we found that hepatoma-derived growth factor (HRP2) was suppressor PIs its downregulation...
Abstract Acquired chemoresistance to proteasome inhibitors is a major obstacle in managing multiple myeloma but key regulators and underlying mechanisms still remain be explored. We find that high level of HP1γ associated with low acetylation modification the bortezomib-resistant cells using SILAC-based acetyl-proteomics assay, higher positively correlated poorer outcomes clinic. Mechanistically, elevated HDAC1 deacetylates at lysine 5 consequently alleviates ubiquitin-mediated protein...
Summary Background Myocardial infarction ( MI ) remains the most common cause of heart failure HF worldwide. For almost 50 years, has been recognized as a determinant adverse prognosis after , but efforts to promote myocardial repair have failed be translated into clinical therapies. Aims In this study, we investigated effects BRD 4 on cardiac function and underlying mechanism. Material Methods The in vivo rat model AMI vitro neonatal cardiomyocytes were established cultured respectively,...
Abstract Myeloma cells produce excessive levels of dickkopf-1 (DKK1), which mediates the inhibition Wnt signaling in osteoblasts, leading to multiple myeloma (MM) bone disease. Nevertheless, precise mechanisms underlying DKK1 overexpression remain incompletely understood. Herein, we provide evidence that hypoxia promotes expression cells. Under hypoxic conditions, p38 kinase phosphorylated cAMP-responsive element-binding protein (CREB) and drove its nuclear import activate transcription. In...
The purpose of the study was to determine roles interleukin-6 (IL6) in fat and bone communication. Male wild-type (WT) mice IL6 knockout (IL6(-/-)) were fed with either regular diet (RD) or high-fat (HFD) for 12 weeks. Bone mass microstructure evaluated by micro-computed tomography. Gene expression related lipid metabolisms assayed real-time quantitative polymerase chain reaction. marrow cells from both genotypes induced differentiate into osteoblasts osteoclasts, treated palmitic acid (PA)....
Abstract Proteasome inhibitors, such as bortezomib (BTZ), represent the key elements in chemotherapy regimens for multiple myeloma (MM), whereas acquired chemoresistance and ultimately relapse remain a major obstacle. In current study, we screened differently expressed cytokines bortezomib-resistant MM cells found that Dickkopf-1 (DKK1) level was remarkably augmented, CD138 significantly suppressed. DKK1 vitro specifically enhanced resistance of to treatment, excessive drove downregulation...
Osteoclastogenesis is a key process in osteolytic bone metastasis (BM). Previous studies indicated that some miRNAs could regulate cancers progression and osteoclastogenesis. Our purpose was to investigate the roles of lung cancer cells-derived circulating miR-21 on osteoclastogenesis its clinical significance BM patients.The difference miRNA expression two cell lines SBC-5 (with characteristic ability) SBC-3 (without were analyzed by microarray qRT-PCR. Circulating levels patients with or...
Abstract Ferroptosis has been demonstrated a promising way to counteract chemoresistance of multiple myeloma (MM), however, roles and mechanism bone marrow stromal cells (BMSCs) in regulating ferroptosis MM remain elusive. Here, we uncovered that were more susceptible ferroptotic induction under the interaction BMSCs using vitro vivo models. Mechanistically, elevated iron level cells, thereby activating steroid biosynthesis pathway, especially production lanosterol, major source reactive...
In multiple myeloma (MM), bone marrow stromal cells (BMSC) shape a unique niche within the marrow, promoting T-cell dysfunction and driving MM progression; however, precise underlying mechanisms remain elusive. Here, we show that BMSC-mediated reprogramming of led to heightened production CXCL10. CXCL10 orchestrated recruitment γδ T into this was observed in both Vk*MYC 5TGM1 mouse models MM, as well patients experiencing refractory or relapsed MM. Furthermore, dysfunctional exhibited...