A5008033769- Gut microbiota and health
- Vibrio bacteria research studies
- Escherichia coli research studies
- Legionella and Acanthamoeba research
- Bacterial biofilms and quorum sensing
- Drug Transport and Resistance Mechanisms
- Diet and metabolism studies
- Bacterial Genetics and Biotechnology
- Clinical Nutrition and Gastroenterology
- Helicobacter pylori-related gastroenterology studies
- Antibiotic Resistance in Bacteria
- Therapeutic Uses of Natural Elements
- Microbial Metabolic Engineering and Bioproduction
- Biosimilars and Bioanalytical Methods
- Medical and Biological Ozone Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Liver Disease Diagnosis and Treatment
- Soil and Environmental Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Oral Health Pathology and Treatment
- Fermentation and Sensory Analysis
- Agriculture and Biological Studies
- Food Quality and Safety Studies
- Computational Drug Discovery Methods
Imperial College London
2016-2021
National Institute for Biological Standards and Control
2021
Medical Research Council
2019
The genus Legionella comprises 65 species, among which pneumophila is a human pathogen causing severe pneumonia. To understand the evolution of an environmental to accidental pathogen, we have functionally analyzed 80 genomes spanning 58 species. Uniquely, immense repository 18,000 secreted proteins encoding 137 different eukaryotic-like domains and over 200 paired with highly conserved type IV secretion system (T4SS). Specifically, show that eukaryotic Rho- Rab-GTPase are found nearly...
The mouse pathogen Citrobacter rodentium is used to model infections with enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC EPEC). Pathogenesis commonly modelled in mice developing mild disease (e.g., C57BL/6). However, little known about host responses exhibiting severe colitis C3H/HeN), which arguably provide a more clinically relevant for human paediatric enteric infection. Infection of C3H/HeN C. results rapid colonic colonisation, coinciding induction key inflammatory...
The gut microbiota plays a crucial role in susceptibility to enteric pathogens, including Citrobacter rodentium, model extracellular mouse pathogen that colonizes the colonic mucosa. C. rodentium infection outcomes vary between strains, with C57BL/6 and C3H/HeN mice clearing succumbing infection, respectively. Kanamycin (Kan) treatment at peak of Kan-resistant resulted relocalization from mucosa cecum solely cecal luminal contents; cessation Kan rapid clearance pathogen. We now show mice,...
Legionella pneumophila causes Legionnaires' disease, a severe form of pneumonia. L. translocates more than 300 effectors into host cells via its Dot/Icm (Defective in organelle trafficking/Intracellular multiplication) type IV secretion system to enable replication target cells. Here, we studied the effector LtpM, which is encoded recombination hot spot Paris. We show that C-terminal phosphoinositol 3-phosphate (PI3P)-binding domain, also found otherwise unrelated effectors, targets LtpM...
Generalised modules for membrane antigens (GMMA)-based vaccines comprise the outer from genetically modified Gram-negative bacteria containing proteins, phospholipids and lipopolysaccharides. Some lipoproteins lipopolysaccharides are pyrogens; thus, GMMA-based intrinsically pyrogenic. It is important to control pyrogenic content of biological medicines, including vaccines, prevent adverse reactions such as febrile responses. The rabbit pyrogen test (RPT) bacterial endotoxin (BET) most...
Bile acid diarrhoea (BAD) is a widespread gastrointestinal disease that often misdiagnosed as irritable bowel syndrome and estimated to affect 1% of the United Kingdom (UK) population alone. BAD associated with excessive bile synthesis secondary or idiopathic disorder (also known primary BAD). Current licensed treatment in UK has undesirable effects been same since was first discovered 1960s. Bacteria are essential transforming acids into acids. The profile an individual’s pool central...
<h3>Introduction</h3> The time it takes for food to travel from the oral cavity until is expelled in stool known as whole-gut transit-time (WGTT). composition of gut microbiome and its metabolites are major contributors health. Indeed, alterations microbiome, bile acid levels, WGTT were reported various gastrointestinal disorders including irritable bowel syndrome, Parkinson's disease, diarrhoea colorectal cancer. However, impact on metabolism has yet be established. <h3>Methods</h3> Healthy...
Significance Legionella pneumophila is a bacterial pathogen causing outbreaks of lethal pneumonia. The genus comprises 65 species for which aquatic amoebae are the natural reservoirs. Using functional and comparative genomics to deconstruct entire we reveal surprising parallel evolutionary trajectories that have led emergence human pathogenic Legionella. An unexpectedly large unique repository secreted proteins (>16,000) containing eukaryotic-like acquired from all domains life (plant,...
Citrobacter rodentium murine infection is the ‘gold standard’ model for investigating host response to human diarrhoeal pathogens, Enteropathogenic and Enterohaemorrhagic Escherichia coli. Mouse strains have varying susceptibility C. with some developing self-resolving mild diarrhoea whereas others develop severe dehydration resulting in lethal disease. One of primary defences against these invading pathogens are intestinal epithelial cells (IECs) which line gastrointestinal tract forming a...