A5060230459- Cancer Cells and Metastasis
- Venomous Animal Envenomation and Studies
- Healthcare and Venom Research
- Respiratory Support and Mechanisms
- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Heme Oxygenase-1 and Carbon Monoxide
- Ion channel regulation and function
- Toxin Mechanisms and Immunotoxins
- Chemical Reactions and Isotopes
- Neonatal Respiratory Health Research
- Cancer Genomics and Diagnostics
- Bacillus and Francisella bacterial research
- Liver physiology and pathology
- Hepatitis B Virus Studies
- Cell death mechanisms and regulation
- Ubiquitin and proteasome pathways
- Cell Image Analysis Techniques
Institut Pasteur Korea
2016-2025
Abstract The intracellular enzyme 11β-hydroxysteroid dehydrogenase type 1 (11βHSD1) catalyzes the interconversion of active glucocorticoid (cortisol) and its intrinsically inert form (cortisone) in metabolic tissues. Although 11βHSD1 is considered a promising therapeutic target disorders such as 2 diabetes, obesity, nonalcoholic steatohepatitis because hepatic functions, roles other tissues have received less attention. In this study, we show that 11βHSD1-specific inhibitor J2H-1702...
Hepatocellular carcinoma (HCC) is the most common form of primary liver cancer in adult. This study aimed to evaluate anticancer potential Naja haje venom subfractions purified by RP-HPLC against HCC. The was performed using two distinct models: a traditional Huh7 cell culture model and 3D involving formation multicellular tumor spheroids (MCTS). were generated co-culturing RFP-Huh7.5 cells with WI38, HUVEC, LX2 cells. Tests also conducted on normal hepatocytes (Fa2N-4). Our results indicate...
Hepatocellular carcinoma (HCC) is one of the most common malignant cancers worldwide and associated with substantial mortality. Because HCCs have strong resistance to conventional chemotherapeutic agents, novel therapeutic strategies are needed improve survival in HCC patients.Here, we developed a fluorescence image-based phenotypic screening system vitro identify HCC-specific drugs co-cultures cells hepatocytes. To this end, identified two distinctive markers HCC, CHALV1 AFP, which highly...
Hepatocellular carcinoma (HCC) is the most common primary liver cancer in adults, fifth malignancy worldwide and third leading cause of related death. An alternative to surgical treatments drugs, such as sorafenib, commonly used medicine necessary overcome this public health problem. In study, we determine anticancer effect on HCC Moroccan cobra Naja haje venom its fraction obtained by gel filtration chromatography against Huh7.5 cell line. Cells were grown together with WI38 human...
Hepatocellular carcinoma (HCC) is the most dominant primary liver cancer, which can be caused by chronic hepatitis virus infections and other environmental factors. Resection, transplantation, local ablation are only a few of highly effective curative procedures presently accessible. However, complementary treatments reduce cancer treatment side effects. In this present work, we evaluated activity Moroccan scorpion venom Buthus occitanus its fractions obtained chromatography gel filtration...
Abstract 3D cell culture system is highlighted as new method to screen for cancer therapies. The multicellular tumor spheroid cultures are closely similar pathophysiological gradients of in vivo tumors and subsequently an ideal screening model drugs. Hepatocellular carcinomas (HCC) resistant conventional chemotherapeutic agents remains unmet medical need. Hence, we focused developing spheroids-based assay identify drugs treat HCC. Tumor were formed hydrogel coated multi-well plates within 3...
Abstract CD133+ cells in hepatocellular carcinoma (HCC) exhibit cancer stem cell (CSC)-like properties as well resistance to chemotherapeutic agents and ionizing radiation; however, their function remains unknown. In this study, we tried define new target compound for enhancement of chemotherapy efficiency HCC through elucidation mechanism CD133-induced chemoresistance. Here, show that strong reactive oxygen species (ROS) via persistent maintenance the ROS-induced increase xCT expression...
Abstract Background: A chronic, local inflammatory milieu can cause tissue fibrosis that results in epithelial-to-mesenchymal transition (EMT), endothelial-to-mesenchymal (EndMT), increased abundance of fibroblasts, and further acceleration fibrosis. Methods: In this study, we aimed to identify potential mechanisms inhibitors using 3D model-based phenotypic screening. We established liver models multicellular tumor spheroids (MCTSs) composed hepatocellular carcinoma (HCC) stromal cells such...