A5017160328- Osteoarthritis Treatment and Mechanisms
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Knee injuries and reconstruction techniques
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- Neuropeptides and Animal Physiology
- Electrical and Bioimpedance Tomography
- Lower Extremity Biomechanics and Pathologies
- Proteoglycans and glycosaminoglycans research
- TGF-β signaling in diseases
- Bone Tissue Engineering Materials
- Redox biology and oxidative stress
- Metabolism, Diabetes, and Cancer
- Graphene and Nanomaterials Applications
- Parathyroid Disorders and Treatments
- Pluripotent Stem Cells Research
- Wound Healing and Treatments
- Pancreatic function and diabetes
- Heat shock proteins research
- Body Composition Measurement Techniques
Kyung Hee University
2014-2023
Kyung Hee University Dental Hospital
2017
National Institute of Dental and Craniofacial Research
2006
Konkuk University
2001
Abstract Germline knockout of the extracellular Ca2+-sensing receptor (CaR) leads to a phenotype that includes severe hypercalcemia, hyperparathyroidism, relative hypocalciuria, skeletal abnormalities, retarded growth, and early postnatal death. To investigate role heterotrimeric G proteins in CaR signaling, we used cre/lox technology delete respective α-subunits Gq G11 selectively parathyroid cells. Mice were PTH-Cre+/−; Gnaqflox/flox; Gna11−/− (PTH-Gαq/Gα11-double knockouts) viable, but...
Because articular chondrocyte-based autologous chondrocyte implantations (ACIs) have restrictively restored cartilage defects, alternative cell sources as a new therapeutic option for repair been introduced.To assess whether implantation of costal chondrocyte-derived pellet-type (CCP) ACI allows safe, functional, and structural restoration full-thickness defects in the knee.Case series; Level evidence, 4.In this first-in-human study, 7 patients with symptomatic, lesions were enrolled. The...
Cell encapsulation has been reported to convey cytoprotective effects and better maintain cell survival. In contrast other studies, our report shows that the deposition of two major biomacromolecules, collagen type I (Col) hyaluronic acid (HA), on mesenchymal stem cells (MSCs) does not entirely block plasma membrane surface. Instead, a considerable amount surface remained uncovered or only slightly covered, as confirmed by TEM observation FACS analysis based quantitative labeling. Despite...
The therapeutic use of ionizing radiation (e.g., X-rays and γ-rays) needs to inflict minimal damage on non-target tissue. Recent studies have shown that substance P (SP) mediates multiple activities in various cell types, including proliferation, anti-apoptotic responses, inflammatory processes. present study investigated the effects SP γ-irradiated bone marrow stem cells (BMSCs). In mouse extracts, prolonged activation Erk1/2 enhanced Bcl-2 expression, but attenuated apoptotic molecules p38...
Given recent progress in regenerative medicine, we need a means to expand chondrocytes quantity without losing their capability. Although many reports have shown that growth factor supplementation can beneficial effects, the use of factor-supplemented basal media has widespread effect on characteristics chondrocytes. Chondrocytes were vitro cultured 2 most widely used chondrocyte media, conventional culture medium and mesenchymal stem cell (MSC) medium, both with fibroblast factor-2 (FGF2)...
Human brain mapping of low-frequency electrical conductivity tensors can realize patient-specific volume conductor models for neuroimaging and stimulation. We report experimental validation in vivo human experiments a new electrodeless tensor imaging (CTI) method. From CTI giant vesicle suspension using 9.4-T MRI scanner, the relative error reconstructed image was found to be less than 1.7% compared with measured value an impedance analyzer. In five subjects were followed 3-T clinical...
Reconstruction of elastic cartilage requires a source chondrocytes that display reliable differentiation tendency. Predetermined tissue progenitor cells are ideal candidates for meeting this need; however, it is difficult to obtain donor because most serves important functions or forms external structures, making these tissues indispensable. We found vestigial in xiphoid processes and characterized as hyaline the proximal region distal region. Xiphoid process-derived (XCs) showed superb...
Near the bone remodeling compartments (BRC), extracellular calcium concentration (Ca2+o) is locally elevated and marrow stromal cells (BMSCs) close to BRC can be exposed high concentration. The calcium-sensing receptor (CaSR) known play a key role in maintaining homeostasis by sensing fluctuations levels of (Ca2+o). When human BMSCs (hBMSCs) were various concentrations (1.8, 3, 5, 10, 30 mM), moderate-high (3–5 mM) stimulated proliferation, while (30 inhibited proliferation. Exposure did not...
Previously, we identified AMRI-59 as a specific pharmaceutical inhibitor of peroxiredoxin (PRX) I enzyme activity. In this study, examined whether acts radiosensitizer in non-small cell lung cancer cells using clonogenic assays. The intracellular mechanisms underlying the radiosensitization effect were determined via immunoblotting addition to measurement ROS generation, mitochondrial potential and death. activity vivo was by co-treating nude mice with compound γ-ionizing radiation (IR),...
Abstract Microcarrier‐based stem cell expansion cultures can increase the dimensions of in vitro from 2D to 3D. The culture handling process then becomes more efficient compared with conventional cultures. However, use spherical plastic microcarriers complicates monitoring culture. To facilitate monitoring, transparent disc‐shaped are manufactured using a light‐initiated microfluidic printing system and obtained named as 2.5D microcarrier. (diameter/height ≈ 5) enable us tools 2D‐based...
Meniscus tissue is composed of highly aligned type I collagen embedded with cartilaginous matrix. This histological feature endows mechanical properties, such as tensile strength along the direction alignment and endurance to compressive load induced by weight bearing. The main objective this study was compare fibrocartilage construction capability different cell sources in presence stimuli.
Non-destructive label-free continuous monitoring of in vitro tissue culture is an unmet demand engineering. Noting that different compositions cartilage lead to electrical properties, we propose a new method measure the conductivity and its anisotropy during chondrogenesis. We used tensor probe with 17 electrodes bio-impedance spectroscopy (BIS) device values ratios at bottom top surfaces samples period 6 weeks. Clearly distinguishing glycosaminoglycans (GAGs), collagen, also various...
Although engineering transplantable tissues for clinical applications is difficult, cryopreserving those to create pre-made engineered tissue emergency transplantation an even greater challenge. It remains uncertain whether the cryopreservation of will become a reality, but if so, it could have significant impact on public health by saving lives and simplifying treatment procedures. All in all, potential implications this innovation far-reaching consequences medical field as whole. This...