A5025816215- Toxoplasma gondii Research Studies
- HIV Research and Treatment
- Ubiquitin and proteasome pathways
- Herpesvirus Infections and Treatments
- Peptidase Inhibition and Analysis
- HIV/AIDS drug development and treatment
- Protein Degradation and Inhibitors
- Autophagy in Disease and Therapy
- Cytomegalovirus and herpesvirus research
- Parasitic Infections and Diagnostics
- Malaria Research and Control
- Cancer-related gene regulation
- Antimicrobial Peptides and Activities
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Sirtuins and Resveratrol in Medicine
- Parasites and Host Interactions
- Cancer-related Molecular Pathways
- Rabies epidemiology and control
- Genomics and Phylogenetic Studies
- Glycosylation and Glycoproteins Research
- Mechanisms of cancer metastasis
University of New Hampshire
2020-2024
Indiana University School of Medicine
2011-2020
Indiana University – Purdue University Indianapolis
2011-2020
Indiana University
2017
ABSTRACT While histone proteins are the founding members of lysine acetylation substrates, it is now clear that hundreds other can be acetylated in multiple compartments cell. Our knowledge scope this modification throughout kingdom life beginning to emerge, as proteome-wide has been documented prokaryotes, Arabidopsis thaliana , Drosophila melanogaster and human cells. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS) identify parasite peptides enriched by immunopurification...
Lysine acetylation has emerged as a major post-translational modification involved in diverse cellular functions. Using combination of immunoisolation and liquid chromatography coupled to accurate mass spectrometry, we determined the first acetylome human malaria parasite Plasmodium falciparum during its active proliferation erythrocytes with 421 sites identified 230 proteins. Lysine-acetylated proteins are distributed nucleus, cytoplasm, mitochondrion apicoplast. Whereas occurrence lysine...
Histone acetylation has been linked to developmental changes in gene expression and is a validated drug target of apicomplexan parasites, but little known about the roles individual histone modifying enzymes how they are recruited genes. The protozoan parasite Toxoplasma gondii (phylum Apicomplexa) unusual among invertebrates possessing two GCN5-family lysine acetyltransferases (KATs). While GCN5a required for response alkaline stress, this KAT dispensable proliferation normal culture...
Toxoplasma gondii is an obligate intracellular parasite of the phylum Apicomplexa, which includes a number species medical and veterinary importance. Inhibitors lysine deacetylases (KDACs) exhibit potent antiparasitic activity, suggesting that interference with acetylation pathways holds promise for future drug targeting. Using high resolution LC-MS/MS to identify peptides enriched by immunopurification acetyl-lysine antibody, we recently produced acetylome proliferative stage Toxoplasma. In...
Lysine acetylation is a critical posttranslational modification that influences protein activity, stability, and binding properties. The of histone proteins in particular well-characterized feature gene expression regulation. In the protozoan parasiteToxoplasma gondii, number lysine acetyltransferases (KATs) contribute to are essential for parasite viability. natural product garcinol was recently reported inhibit enzymatic activities GCN5 p300 family KATs other species. Here we show inhibits...
The ATPase family AAA+ domain containing 2 (ATAD2) protein and its paralog ATAD2B have a C-terminal bromodomain (BRD) that functions as reader of acetylated lysine residues on histone proteins. Using structure–function approach, we investigated the ability ATAD2/B BRDs to select among multiple post-translational modifications. BRD can bind ligands also contain adjacent methylation or phosphorylation marks, while presence these modifications significantly weakened acetyllysine binding...
Lysine acetylation is a reversible post-translational modification (PTM) that has been detected on thousands of proteins in nearly all cellular compartments. The role this widespread PTM yet to be fully elucidated, but can impact protein localization, interactions, activity, and stability. Here we present the first proteome-wide survey lysine cortical astrocytes, subtype glia component blood-brain barrier key regulator neuronal function plasticity. We identified 529 sites across 304 found...
New drugs to control infection with the protozoan parasite Toxoplasma gondii are needed as current treatments exert toxic side effects on patients. Approaches develop novel compounds for drug development include screening of compound libraries and targeted inhibition essential cellular pathways. We identified two distinct that display inhibitory activity against parasite's replicative stage: F3215-0002, which we previously during a library screen, I-BET151, an inhibitor bromodomains,...
The protozoan pathogen Toxoplasma gondii relies on tight regulation of gene expression to invade and establish infection in its host. divergent regulatory mechanisms related apicomplexan pathogens rely heavily regulators chromatin structure histone modifications. important contribution acetylation for both acute chronic has been demonstrated, where increases at active loci. However, the direct consequences specific marks pathway that influences transcriptional response modification are...
Toxoplasma gondii is a foodborne pathogen that can cause severe and life-threatening infections in fetuses immunocompromised patients. Felids are its only definitive hosts, wide range of animals, including humans, serve as intermediate hosts. When the transmissible bradyzoite stage orally ingested by felids, they transform into merozoites expand asexually, ultimately generating millions gametes for parasite sexual cycle. However, bradyzoites hosts differentiate exclusively to disease-causing...
// Stephan J. Matissek 1 , Mona Karbalivand Weiguo Han Ava Boutilier Estefania Yzar-Garcia Laura L. Kehoe Devin Storm Gardner Adam Hage 2 Krista Fleck Vicki Jeffers Ricardo Rajsbaum 3 and Sherine F. Elsawa Department of Molecular, Cellular Biomedical Sciences, University New Hampshire, Durham, NH, USA Microbiology Immunology, Texas Medical Branch, Galveston, TX, Institute for Human Infections Immunity, Correspondence to: Elsawa, email: sherine.elsawa@unh.edu Keywords: GLI3; inflammation; TLR...
ABSTRACT Toxoplasma gondii is a foodborne pathogen that can cause severe and life-threatening infections in fetuses immunocompromised patients. Felids are its only definitive hosts, wide range of animals, including humans, serve as intermediate hosts. When the transmissible bradyzoite stage orally ingested by felids, they transform into merozoites expand asexually, ultimately generating millions gametes for parasite sexual cycle. However, bradyzoites hosts differentiate exclusively to...