A5056172762- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Machine Learning in Bioinformatics
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Hemoglobinopathies and Related Disorders
- Protein Structure and Dynamics
- Iron Metabolism and Disorders
- Immunotherapy and Immune Responses
- Erythropoietin and Anemia Treatment
- Bioinformatics and Genomic Networks
- Trace Elements in Health
- Advanced NMR Techniques and Applications
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2019-2024
University of Warsaw
2019
Abstract Low complexity regions (LCRs) in protein sequences are characterized by a less diverse amino acid composition compared to typically observed sequence diversity. Recent studies have shown that LCRs may co-occur with intrinsically disordered regions, highly conserved many organisms, and often play important roles functions diseases. In previous decades, several methods been developed identify or bias, but most of them as stand-alone applications currently there is no web-based tool...
Low complexity regions are fragments of protein sequences composed only a few types amino acids. These frequently occur in proteins and can play an important role their functions. However, scientists mainly focused on characterized by high diversity acid composition. Similarity between reflect functional similarity them. In this article, we discuss strengths weaknesses the analysis low using BLAST, HHblits CD-HIT. methods considered to be gold standard were designed for comparison regions....
Electrostatic interactions play important roles in the functional mechanisms exploited by intrinsically disordered proteins (IDPs). The atomic resolution description of long-range and local structural propensities that can both be crucial for function highly charged IDPs presents significant experimental challenges. Here, we investigate conformational behavior δ subunit RNA polymerase from Bacillus subtilis whose unfolded domain is charged, with 7 positively amino acids followed 51 acidic...
Abstract Background The rapid spread of the COVID-19 demands immediate response from scientific communities. Appropriate countermeasures mean thoughtful and educated choice viral targets (epitopes). There are several articles that discuss such choices in SARS-CoV-2 proteome, other focus on phylogenetic traits history Coronaviridae genome/proteome. However none consider protein low complexity regions (LCRs). Recently we created first methods able to compare fragments. Results We show five...
Abstract Low Complexity Regions (LCRs) are segments of proteins with a low diversity amino acid composition. These regions play important roles in proteins. However, annotations describing these functions dispersed across databases and scientific literature. LCRAnnotationsDB aims to consolidate knowledge about LCRs store relevant single place. To unify redundant annotations, we assigned them categories based on similarity function, protein structure, biological process. Categories organized...
Hepcidin is an iron regulatory hormone that does not bind directly. Instead, its mature 25-peptide form (H25) contains a binding site for other metals, the so-called ATCUN/NTS (amino-terminal Cu/Ni site). The Cu(II)-hepcidin complex was previously studied, but due to poor solubility and difficult handling of peptide definitive account on equilibrium obtained reliably. In this study we performed series fluorescence competition experiments between H25 model peptides containing same determined...
Abstract The rapid spread of the COVID-19 demands immediate response from scientific communities. Appropriate countermeasures mean thoughtful and educated choice viral targets (epitopes). There are several articles that discuss such choices in SARS-CoV-2 proteome, other focus on phylogenetic traits history Coronaviridae genome/proteome. However none consider protein low complexity regions (LCRs). Recently we created first methods able to compare fragments. We show five (LCRs) three proteins...
Hepcidin is an iron regulatory hormone that does not bind directly. Instead, its mature 25-peptide form (H25) contains a binding site for other metals, the so-called ATCUN/NTS (amino-terminal Cu/Ni site). The Cu(II)-hepcidin complex was previously studied, but due to poor solubility and difficult handling of peptide definitive account on equilibrium obtained reliably. In this study we performed series fluorescence competition experiments between H25 model peptides containing same determined...