A5091207829- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Bacteriophages and microbial interactions
- Cancer-related molecular mechanisms research
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Saffron Plant Research Studies
- Plant Molecular Biology Research
- Genomics and Rare Diseases
- Plant tissue culture and regeneration
- Advanced biosensing and bioanalysis techniques
- Enzyme Structure and Function
- Microbial Natural Products and Biosynthesis
- Metabolism and Genetic Disorders
- Viral Infections and Immunology Research
- RNA regulation and disease
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Plant Reproductive Biology
- Marine Ecology and Invasive Species
- Escherichia coli research studies
- Polyamine Metabolism and Applications
Hirosaki University
2011-2024
Iwate University
1999-2021
Tsinghua University
2013
Kobe University
2010
Japan Science and Technology Agency
2010
Fukuyama University
2008
Tokyo University of Science
2007
Institute of Space and Astronautical Science
1989-1995
The University of Tokyo
1984-1995
Tokyo Institute of Technology
1990
All sequenced histidine tRNAs have one additional nucleotide at the 5' end compared with other tRNA species. To investigate role of this unique structure in aminoacylation, we constructed vitro transcripts corresponding to E. coli sequence and its variants G-1-C73 base pair, by using T7 RNA polymerase transcription system. A transcript having a wild-type no modified bases was good substrate for histidyl-tRNA synthetase (HisRS), aminoacylation activity affected introduction triphosphate...
The stable RNAs, whose sequences are homologous to 10Sa RNA of Escherichia coli , have been isolated from Mycoplasma capricolum and Bacillus subtilis both belonging the Gram-positive bacterial group. total nucleotide RNAs determined by partial sequencing DNA their genes. A comparison sequences, together with those other so far known, has shown that 5′- 3′-end well conserved among species, while central parts reveal little homologies. Unexpectedly, 3′-regions can be folded in a common...
Journal Article In vitro study of E.coli tRNAArg and tRNALys identity elements Get access Koji Tamura, Tamura Institute Space Astronautical Science3-1-1 Yoshinodai, Sagamihara, Kanagawa 229, Japan Search for other works by this author on: Oxford Academic PubMed Google Scholar Hyouta Himeno, Himeno Haruichi Asahara, Asahara Tsunemi Hasegawa, Hasegawa Mikio Shimizu * *To whom correspondence should be addressed Nucleic Acids Research, Volume 20, Issue 9, 11 May 1992, Pages 2335–2339,...
Journal Article Conversion of aminoacylation specificity from tRNA Tyr to Serin vitro Get access Hyouta Himeno, Himeno * To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Tsunemi Hasegawa, Hasegawa Takuya Ueda, Ueda 1Department Biological Sciences, Faculty Bioscience and Biotechnology, Tokyo Institute Technology4259 Nagatsuta, Midori-ku, Yokohama 227, Japan Kimitsuna Watanabe, Watanabe Mikio Shimizu Nucleic Acids...
Crocin, picrocrocin, and safranal as the major secondary metabolites are important for high quality of spice saffron. Crocin picrocrocin were detected in stigma-like structures proliferated vitro. Safranal appeared after heat treatment. The contents relative ratio these substances similar to those intact young stigmas, indicating that showed not only morphological but also biochemical similarity stigmas.
Ribosome biogenesis is a tightly regulated, multi-stepped process. The assembly of ribosomal subunits central step the complex process, involving nearly 30 protein factors in vivo bacteria. Although process has been extensively studied vitro for over 40 years, very limited information known and specific roles factors. Such an example ribosome maturation factor M (RimM), involved late-stage 30S subunit. Here, we combined quantitative mass spectrometry cryo-electron microscopy to characterize...
Bacterial transfer-messenger RNA (tmRNA, 10Sa RNA) is involved in a trans-translation reaction which contributes to the degradation of incompletely synthesized peptides and recycling stalled ribosomes. However, its physiological role cell remains elusive. In this study, an efficient system for controlling expression gene tmRNA (ssrA), as well gene-defective strain (ssrA:cat), were constructed Bacillus subtilis. The effects on growth cells investigated under various culture conditions using...
The GTPase activity of Escherichia coli YjeQ, here named RsgA (ribosome small subunit-dependent A), has been shown to be significantly enhanced by ribosome or its subunit. enhancement was inhibited several aminoglycosides bound at the A site subunit, but not a P site-specific antibiotic. stably subunit in presence GDPNP, GTP GDP, dissociate into subunits. Disruption gene for from genome affected growth cells, which predominantly contained dissociated subunits having only weak activation...
Abstract The 16,260-bp mitochondrial DNA (mtDNA) from the starfish Asterina pectinifera has been sequenced. genes for 13 proteins, two rRNAs and 22 tRNAs are organized in an extremely economical fashion, similar to those of other animal mtDNAs, with some overlapping each other. gene organization is same as that another echinoderm, sea urchin, except inversion a 4.6-kb segment contains 16S rRNA. Judging protein coding genes, mammalian mtDNAs resemble urchin mtDNA more than starfish. region...
A novel translation, trans ‐translation, is facilitated by a highly structured RNA molecule, tmRNA. This molecule has two structural domains, tRNA domain and an mRNA domain, the latter including four pseudoknot structures (PK1 to PK4). Here, we show that replacement of each these pseudoknots, except PK1, in Escherichia coli tmRNA with single stranded did not seriously affect functions as alanine mRNA. Furthermore, three pseudoknots were interchangeable only small losses functions. These...
Recognition of tRNA His by Saccharomyces corevisiae histidyl-tRNA synthetase was studied using in vitro transcripts. Histidine is unique possessing an extra nucleotide, G 1 , at the 5′ end. Mutation studies indicate that this irregular secondary structure end acceptor stem important for aminoacylation with histidine, while requirement either base pair smaiier than Escherichia coli . The anticodon also found to be required histidylation. regions involved histidyiation are essentially same as...
Journal Article Unilateral Aminoacylation Specificity between Bovine Mitochondria and Eubacteria Get access Yoshinori Kumazawa, Kumazawa *Department of Biological Sciences, Tokyo Institute TechnologyNagatsuta, Midori-ku, Yokohama, Kanagawa 227 Search for other works by this author on: Oxford Academic PubMed Google Scholar Hyouta Himeno, Himeno **Institute Space Astronautical ScienceSagamihara, 229 Kin-ichiro Miura, Miura ***Department Industrial Chemistry, Faculty Engineering, The University...
Although trans-translation mediated by tmRNA-SmpB has long been known as the sole system to relieve bacterial stalled ribosomes, ArfA recently identified an alternative factor for ribosome rescue in Escherichia coli. This process requires hydrolysis of nascent peptidyl-tRNA RF2, which usually acts a stop codon-specific peptide release factor. It poses fascinating question how and RF2 recognize ribosome. Here, we mapped location directed hydroxyl radical probing. revealed ArfA-binding site...