A5102793365- MicroRNA in disease regulation
- Plant Stress Responses and Tolerance
- GABA and Rice Research
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Plant Gene Expression Analysis
- Fermentation and Sensory Analysis
- Plant biochemistry and biosynthesis
- Cancer-related molecular mechanisms research
- Plant Virus Research Studies
- Microbial Metabolic Engineering and Bioproduction
- Advanced Chemical Sensor Technologies
- Insect-Plant Interactions and Control
- Phytochemicals and Antioxidant Activities
- Aquaculture disease management and microbiota
- Plant tissue culture and regeneration
- melanin and skin pigmentation
- Gold and Silver Nanoparticles Synthesis and Applications
- interferon and immune responses
- Biochemical and biochemical processes
- Probiotics and Fermented Foods
- Anaerobic Digestion and Biogas Production
- Gut microbiota and health
- Cancer-related gene regulation
- Physiological and biochemical adaptations
Gansu Agricultural University
2022-2025
Yunnan Academy of Agricultural Sciences
2019-2024
Tobacco Research Institute
2019-2022
Charles River Laboratories (United States)
2021
National Center for Gene Research
2020
Abstract Background Anthocyanins determinate the flower color of many plants. Tobacco is a model plant for studying molecular regulation coloration. We investigated mechanism underlying coloration in tobacco by profiling flavonoid metabolites,expression anthocyanin biosynthetic structural genes and their regulator pink-flowered cultivar Yunyan 87 white-flowered mutant. Result Significant down-accumulation anthocyanins, including cyanidin 3-O-glucoside, cyanin, 3-O-rutinoside, pelargonidin...
Leaf stacking fermentation involves enzymatic actions of many microorganisms and is an efficient environmentally benign process for degrading macromolecular organic compounds. We investigated the dynamics metabolite profiles, bacterial fungal communities their interactions during using cigar leaves from three geographic regions. The results showed that contents total sugar, reducing starch, cellulose, lignin, pectin, polyphenol protein in tobacco was significantly decreased fermentation....
Heated tobacco (Nicotiana tabacum L.) products develop rapidly because of its low toxicity. In order to evaluate the industrial applicability existing types characteristic as raw materials for heated tobaccos, this research ascertained differences in main chemical compositions leaves from different establish a relationship between composition and sensory evaluation select suitable producing tobaccos. Nine (K326, Yunyan 97, Cherry-red tobacco, Han Dao Tiandeng Sun-cured yellow fresh-cut...
Stomatal movement can be regulated by ABA signaling through synthesis of reactive oxygen species (ROS) in guard cells. By contrast, ethylene triggers the biosynthesis antioxidant flavonols to suppress ROS accumulation and prevent ABA-induced stomatal closure; however, underlying mechanism remains largely unknown. In this study, we isolated characterized tobacco (Nicotiana tabacum) R2R3-MYB transcription factor NtMYB184, which belongs flavonol-specific SG7 subgroup. RNAi suppression...
The improvement in poultry production performance varies with different microbial strains used fermented feed. This study investigates the efficacy of Lactiplantibacillus-plantarum-ZG7-fermented feed (ZG7-FF) on productivity laying hens. Results indicated that ZG7-FF significantly reduced daily intake while increasing egg weight and decreasing feed-to-egg ratio during peak (p < 0.05), addition to enhancing late-phase rate 0.05). Further intestinal morphological results showed increased...
Proanthocyanidins (PAs) are important phenolic compounds and PA biosynthesis is regulated by a ternary MBW complex consisting of R2R3-MYB regulator, bHLH factor WDR protein. In this study, tobacco NtMYB330 was characterized as the PA-specific regulator in which promoted flowers NtMYB330-overexpressing lines while decreased ntmyb330 mutants. can interact with flavonoid-related partner NtAn1b protein NtAn11-1, NtMYB330-NtAn1b required to achieve strong transcriptional activation PA-related...
Tobacco alkaloid metabolism is regulated by various transcription factors (TFs). Here, we have characterized a non-NIC2 locus gene, Ethylene Response Factor 91 (ERF91), function in regulation of accumulation tobacco. NtERF91 was preferentially expressed roots and induced jasmonic acid. Additionally, able to vitro bind the NtPMT2 NtQPT2 promoters via directly targeting GCC-box elements transactivate gene expression. Ectopic overexpression not only increased expression most nicotine...
Heated tobacco (Nicotiana tabacum L.) products are heating plug at a temperature of 350°C and produce different emissions in aerosol sensory perceptions leaf compared with combustible tobacco. Previous study assessed varieties heated for quality analyzed the links between scores final certain chemical classes leaf. However, contribution individual metabolites to remains largely open investigation.In present study, five were evaluated as by an expert panel volatile non-volatile non-targeted...
Surface enhanced Raman spectroscopy (SERS) is a unique analytical technique with excellent performance in terms of sensitivity, non-destructive detection and resolution. However, due to the randomness poor repeatability hot spot distribution, SERS quantitative analysis still challenging. Meanwhile, snus type tobacco product that can release nicotine other components mouth without burning, rapid based on reliably evaluate amount released from snus, which great significance for understanding...
The biosynthesis and transport of nicotine has been shown to be coordinately upregulated by jasmonate (JA). MYC2, a member basic helix-loop-helix (bHLH) transcription factor family, is well-documented as the core player in JA signalling pathway regulate diverse plant development processes. Four MYC2 genes were found tobacco genome, NtMYC2a/2b 1a/1b. In this study, we tested whether one them, NtMYC2a, acts 'master switch' regulation tobacco. We generated NtMYC2a knockout plants using...
Introduction Carotenoids are important precursors of various aroma components in tobacco and play an role the sensory quality tobacco. Phaffia rhodozyma is a species Xanthophyllomyces capable synthesizing highly valuable carotenoid-astaxanthin, but has not yet been used improving quality. Methods The dynamic changes microbial community metabolites during fermentation were analyzed combination with microbiome metabolome, after was evaluated by scores. Results P. could grow produce carotenoids...
We previously found that DNA methyltransferase 3a (DNMT3a) plays an important role in regulating embryonic cardiomyocyte gene expression, morphology, and function. In this study, we investigated the of most abundant DNMT mammalian cells, DNMT1, these processes. It is known DNMT1 essential for development, during which it involved methylation expression. used siRNA to knock down expression primary cultures mouse cardiomyocytes. Immunofluorescence staining multielectrode array were,...
The volatile release evaluations of nicotine snus products under different storage conditions were performed based on Fe 3 O 4 microparticles coated with Au nanorods and nanoparticles (Fe @AuNRsNPs) as SERS substrates combined capillary.
Recessive resistance against potyviruses, such as Potato virus Y (PVY), relies on mutations in the eukaryotic translation initiation factor 4E (eIF4E) or one of its isoforms. The eIF4E1-S mutants burley tobacco (Nicotiana tabacum L.) exhibit recessive PVY strains. Here, we developed a TILLING population flue-cured (N. cv. Yunyan87) using ethyl methanesulfonate (EMS) to identify mutants. M3 plants homozygous for nonsense mutation exon 1 gene demonstrated PVYMN. These were backcrossed...
Substantial progress had been made in reducing nornicotine accumulation burley tobacco, as is a precursor of the carcinogen N-nitrosonornicotine (NNN). Three members CYP82E2 family encoding nicotine N-demethylase (NND) have reported to be responsible for majority demethylation that forms tobacco. We obtained nonsense mutant each NND member flue-cured tobacco from an ethyl methanesulfonate (EMS)-mutagenized population. In this study, we developed dCAPS markers mutation. Using marker-assisted...
Introduction: Different types of tobacco cultivars have been used as raw material in heated to maximize the sensory richness. However, volatile profiles different and their correlation with quality remain unknown. Method: In this study, untargeted metabolomics profiling followed by partial least squaresdiscriminant analysis (VIP>1 p<0.05) was performed identify 446 445 metabolites that were statistically among three reconstituted leaf aerosol, respectively. Flue-cured K326 rated...