A5081169678- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Single-cell and spatial transcriptomics
- Cancer-related molecular mechanisms research
- Cardiovascular Disease and Adiposity
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Genetic and phenotypic traits in livestock
- Muscle Physiology and Disorders
- Mast cells and histamine
- Pancreatitis Pathology and Treatment
- Mitochondrial Function and Pathology
- Diet and metabolism studies
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- Immune cells in cancer
- Dietary Effects on Health
- Body Contouring and Surgery
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Gender Roles and Identity Studies
- Fibroblast Growth Factor Research
- IL-33, ST2, and ILC Pathways
- Lymphatic System and Diseases
- Obesity and Health Practices
- Epigenetics and DNA Methylation
Translational Research Institute for Metabolism and Diabetes
2012-2025
AdventHealth Orlando
2020-2025
Translational Research Institute
2023-2024
Sanford Burnham Prebys Medical Discovery Institute
2012-2017
Discovery Institute
2016-2017
Sorbonne Université
2008-2014
Fondation pour l’innovation en Cadiométabolisme et Nutrition
2012-2014
Pitié-Salpêtrière Hospital
2014
Inserm
2008-2012
Centre de Recherche des Cordeliers
2008-2012
OBJECTIVE Fibrosis is a newly appreciated hallmark of the pathological alteration human white adipose tissue (WAT). We investigated composition subcutaneous (scWAT) and omental WAT (oWAT) fibrosis in obesity its relationship with metabolic alterations surgery-induced weight loss. RESEARCH DESIGN AND METHODS Surgical biopsies for scWAT oWAT were obtained 65 obese (BMI 48.2 ± 0.8 kg/m2) 9 lean subjects 22.8 0.7 kg/m2). Obese who candidates bariatric surgery clinically characterized before, 3,...
Chemerin is a new adipokine involved in vitro adipogenesis and insulin resistance associates with body mass index (BMI) vivo.We investigated the role of chemerin morbid obesity, associated metabolic diseases (insulin resistance, hepatic diseases), postsurgery-induced weight loss.This was prospective study performed at university hospital.Subjects included 60 obese female patients (BMI 50.0 +/- 1.0 kg/m(-2)) being candidates for gastric bypass.Patients were examined before 3, 6, 12 months...
To examine the role of adipose-produced chemokine, chemokine ligand (CCL) 5, on recruitment and survival macrophages in human white adipose tissue (WAT).CCL5 levels measured by enzyme immunoassay serum real-time polymerase chain reaction WAT were higher obese compared to lean subjects. CCL5, but not CCL2, secretion was visceral subcutaneous WAT. CCL5 mRNA expression positively correlated with inflammatory macrophage markers as CD11b, tumor necrosis factor-alpha, IL-6 (n=24 subjects), than...
Fat distribution differs in men and women, but both sexes, a predominantly gluteal-femoral compared with abdominal (central) fat is associated lower metabolic risk. Differences cellular characteristics functions of these depots have been described, the molecular mechanisms involved are not understood.Our objective was to identify depot- sex-dependent differences gene expression human gluteal sc adipose tissues.Abdominal tissue aspirates were obtained from 14 premenopausal women [age 27.5 ±...
Mast cells are immune known for their role in several inflammatory and fibrotic diseases. Recent works mice suggest that mast could be cellular actors involved the pathophysiology of obesity, a disease characterized by white adipose tissue (WAT) systemic inflammation. The aim study was to better characterize WAT obese with or without type 2 diabetes lean subjects as well explore relationship inflammation fibrosis.Subcutaneous omental from six subjects, 10 nondiabetic, diabetic patients...
Objective Peripheral lower body fat is associated with cardiometabolic risk. Physiological differences in gluteal compared abdominal subcutaneous (sc) adipocyte functions are known but the molecular basis for depot function poorly understood. Our goal to identify novel gene regulatory pathways that underlie heterogeneity of human distribution. Methods Abdominal and adipose tissue aspirates obtained from 35 subjects (age = 30 ± 1.6 years; BMI 27.3 1.3 kg/m 2 ) were analyzed using Illumina...
White adipose tissue (WAT) is a complex mixture of adipocytes and non-adipogenic cells. Characterizing the cellular composition WAT critical for identifying where potential alterations occur that impact metabolism. Most single-cell (sc) RNA-Seq studies focused on stromal vascular fraction (SVF) which does not contain have used technology has 3' or 5' bias. Using full-length sc/single-nuclei (sn) technology, we interrogated transcriptional using: snRNA-Seq whole tissue, isolated adipocytes,...
Adipose-derived stem cells (ADSCs) play an important role in the differential capacity for excess energy storage between upper body abdominal (ABD) adipose tissue (AT) and lower gluteofemoral (GF) AT. We cultured ADSCs from subcutaneous ABD AT GF isolated eight women with fat distribution performed single-cell RNA sequencing. Six populations of were identified segregated according to their anatomical origin. The three ADSC subpopulations characterized by strong cholesterol/fatty acid (FA)...
Context: Acute phase serum amyloid A (A-SAA) is secreted by hepatocytes in response to injury and regulated proinflammatory cytokines. In obese humans, adipocytes are also a major contributor circulating A-SAA levels.
Extracellular matrix (ECM) remodeling is essential for adipose tissue growth and expansion in high fat-fed mice, there evidence of fibrosis human obesity.The aim the study was to explore role ECM healthy, growing children. RESEARCH DESIGN, SETTING, AND PARTICIPANTS: Abdominal sc biopsies were obtained from 65 otherwise healthy children [57 boys; age, 5.3 ± 3.8 yr (mean sd)] having elective surgery (cross-sectional study). Twenty percent participants classified as overweight/obese based on...
Significance We report an essential regulator of insulin sensitivity. Mutations affecting this protein, KBTBD2, cause severe insulin-resistant diabetes, lipodystrophy, hepatic steatosis, and growth retardation. KBTBD2 is the substrate recognition subunit a ubiquitin ligase, its molecular target p85α, regulatory phosphoinositol-3-kinase. highly conserved among vertebrates expressed in liver, brain, muscle, adipocytes. In absence p85α levels rise 30-fold adipocytes, interrupting signal, which...
Abstract White adipose tissue (WAT) is a robust energy storage and endocrine organ critical for maintaining metabolic health as we age. Our aim was to identify cell‐specific transcriptional aberrations that occur in WAT with aging. We leveraged full‐length snRNA‐Seq histology characterize the cellular landscape of human abdominal subcutaneous prospective cohort 10 younger (≤30 years) older individuals (≥65 balanced sex body mass index (BMI). The group had greater cholesterol,...
Cellular heterogeneity of human adipose tissue is linked to the pathophysiology obesity and may impact response energy restriction changes in fat mass. Herein, we provide an optimized pipeline estimate cellular composition abdominal subcutaneous (ASAT) bulk RNA sequencing (RNA-seq) datasets using a single-nuclei RNA-seq signature matrix. A deconvolution for ASAT was by benchmarking publicly available algorithms matrix derived from data 20 adults then applied cell-type proportions weight loss...
Polycystic ovary syndrome (PCOS) is often associated with metabolic features, including central obesity, suggesting that adipose tissue (AT) a key organ in PCOS pathobiology. In this study, we compared both abdominal (ABD) and gluteofemoral (GF) subcutaneous AT women without PCOS. ABD GF ATs from BMI/WHR-matched control were analyzed by RT-qPCR, FACS histology. adipose-derived stem cell (ASC) transcriptome methylome RNA-seq DNA methylation array. Similar to the group of showed lower...
Objective Human brown adipose tissue (BAT) activity decreases with age and obesity. In addition to uncoupling protein 1 (UCP1), several genetic markers of BAT in humans have been published. However, the link between human has inadequately explored. Methods White (WAT) biopsies were obtained from 16 patients undergoing deep neck surgery. vitro differentiated adipocytes used measure norepinephrine‐stimulated mitochondrial as a activity. Gene expression was determined biopsies. Results...
Increased lower body fat is associated with reduced cardiometabolic risk. The molecular basis for depot-specific differences in gluteofemoral (GF) compared abdominal (A) subcutaneous adipocyte function poorly understood. In the current report, we used a combination of Assay Transposase-Accessible Chromatin followed by sequencing (ATAC-seq), RNA-seq, and chromatin immunoprecipitation (ChIP)-qPCR analyses that provide evidence gene expression patterns are differential epigenetic signatures....
Insulin resistance and blunted mitochondrial capacity in skeletal muscle are often synonymous, however, this association remains controversial. The aim of study was to perform an in-depth multifactorial comparison between individuals who were lean active (Active,
Objective Lower body fat is associated with diminishing cardiometabolic risk. Physiological differences between gluteofemoral and abdominal subcutaneous adipocyte functions are known, but the molecular basis for depot in function poorly understood. The objective of this study was to identify microRNA (miRNA) expression human adipose tissues their implication gene regulation. Methods Abdominal tissue aspirates obtained from 18 participants (9 male 9 female, age 30 ± 1.5 y, BMI 27.3 1.23 kg/m...
Accumulation of fat above the waist is an important risk factor in developing obesity-related comorbidities independently BMI or total mass. Deciphering gene regulatory programs adipose tissue precursor cells within upper body abdominal (ABD) and lower gluteofemoral (GF) depots to understand their differential capacity for lipid accumulation, maturation, disease risk. Previous studies identified HOX transcript antisense intergenic RNA (HOTAIR) as a GF-specific lncRNA; however, its role...
Abstract Background Preferential accumulation of fat in the upper body (apple shape) is associated with higher risk developing metabolic syndrome relative to lower (pear shape). We previously discovered that chromatin openness partially defined transcriptome preadipocytes isolated from abdominal and gluteofemoral fat. However, molecular mechanisms underlying interindividual variation shape are unknown. Methods Adipocyte fraction was biopsies premenopausal women (age mass index matched)...
Automated single-cell dispensing is incompatible with white adipose tissue (WAT) due to lipid-laden adipocytes. Single-nuclei RNA-Seq permits transcriptional profiling of all cells from WAT. Human WAT faces unique technical challenges in isolating nuclei compared rodent greater extra-cellular matrix content and larger lipid droplets. In this protocol, we detail how isolate frozen subcutaneous human for single-nuclei RNA-Seq. For complete information on the generation use please refer Whytock...