A5075282722- Adipose Tissue and Metabolism
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Nutrition, Genetics, and Disease
- Body Composition Measurement Techniques
- Pancreatic function and diabetes
- Diet and metabolism studies
- Metabolism, Diabetes, and Cancer
- Attention Deficit Hyperactivity Disorder
- Redox biology and oxidative stress
- Gene expression and cancer classification
- Muscle Physiology and Disorders
- Cardiovascular Disease and Adiposity
- Nutrition and Health in Aging
- Thermal and Kinetic Analysis
- Computational Drug Discovery Methods
- Genetic Associations and Epidemiology
- Cardiovascular and exercise physiology
- Traumatic Brain Injury Research
- Genomics, phytochemicals, and oxidative stress
- Renal Transplantation Outcomes and Treatments
- Hemoglobin structure and function
- Chemical Thermodynamics and Molecular Structure
- Cancer therapeutics and mechanisms
Translational Research Institute for Metabolism and Diabetes
2022-2025
Translational Research Institute
2023-2024
AdventHealth Orlando
2023-2024
Centenary Institute
2018-2020
The University of Sydney
2017-2020
UNSW Sydney
2015-2020
National Health and Medical Research Council
2020
Children's Hospital at Westmead
2017
New Mexico Regional Federal Medical Center
2000
Boston VA Research Institute
1984
Abstract Protein oxidation sits at the intersection of multiple signalling pathways, yet magnitude and extent crosstalk between other post-translational modifications remains unclear. Here, we delineate global changes in adipocyte networks following acute oxidative stress reveal considerable cysteine phosphorylation-based signalling. Oxidation key regulatory kinases, including Akt, mTOR AMPK influences fidelity rather than their absolute activation state, highlighting an unappreciated...
Inter-organ communication is a vital process to maintain physiologic homeostasis, and its dysregulation contributes many human diseases. Given that circulating bioactive factors are stable in serum, occur naturally, easily assayed from blood, they present obvious focal molecules for therapeutic intervention biomarker development. Recently, studies have shown secreted proteins mediating inter-tissue signaling could be identified by 'brute force' surveys of all genes within RNA-sequencing...
Abstract Objective The aim of this study was to examine associations ectopic adipose tissue (AT) with skeletal muscle (SM) mitochondrial bioenergetics in older adults. Methods Cross‐sectional data from 829 adults ≥70 years age were used. Abdominal, subcutaneous, and visceral AT thigh fat infiltration (MFI) quantified by magnetic resonance imaging. SM energetics characterized vivo ( 31 P‐magnetic spectroscopy; ATP max ) ex (high‐resolution respirometry maximal oxidative phosphorylation...
Age-related changes in adipose tissue impact chronic medical diseases and mobility disability but mechanism remains poorly understood. The goal of this study is to define methods for phenotyping unique characteristics from older adults. Older adults enrolled Study Muscle, Mobility Aging selected the ancillary (SOMMA-AT; N=210, 52.38% women, 76.12±4.37 years) were assessed regional adiposity by whole-body magnetic resonance (AMRA) underwent a needle-aspiration biopsy abdominal subcutaneous...
Insulin resistance and blunted mitochondrial capacity in skeletal muscle are often synonymous, however, this association remains controversial. The aim of study was to perform an in-depth multifactorial comparison between individuals who were lean active (Active,
Protein disulfide bonds link pairs of cysteine sulfur atoms and are either structural or functional motifs. The allosteric disulfides control the function protein in which they reside when cleaved formed. Here, we identify potential all Data Bank X-ray structures from that present some molecules a crystal but absent others, others. We reasoned labile nature these signifies propensity for cleavage so possible regulation bond resides. A total 511 were identified. more stressed than average...
Automated single-cell dispensing is incompatible with white adipose tissue (WAT) due to lipid-laden adipocytes. Single-nuclei RNA-Seq permits transcriptional profiling of all cells from WAT. Human WAT faces unique technical challenges in isolating nuclei compared rodent greater extra-cellular matrix content and larger lipid droplets. In this protocol, we detail how isolate frozen subcutaneous human for single-nuclei RNA-Seq. For complete information on the generation use please refer Whytock...
The impact of aerobic training on human skeletal muscle cell (HSkMC) mitochondrial metabolism is a significant research gap, critical to understanding the mechanisms by which exercise augments metabolism. We therefore assessed content and capacity in fully differentiated CD56 + HSkMCs from lean active (LA) sedentary individuals with obesity (OS) at baseline, as well lean/overweight (LOS) baseline following an 18-day intervention. Participants had vivo PCr recovery rate 31 P-MRS...
Inter-organ communication is a vital process to maintain physiologic homeostasis, and its dysregulation contributes many human diseases. Given that circulating bioactive factors are stable in serum, occur naturally, easily assayed from blood, they present obvious focal molecules for therapeutic intervention biomarker development. Recently, studies have shown secreted proteins mediating inter-tissue signaling could be identified by ‘brute force’ surveys of all genes within RNA-sequencing...
Abstract Disclosure: M. Zhou: None. I. Tamburini: C. Van: J. Molendijk: L.M. Velez: Nguyen: R. Yeo: Filho: A.L. Hevener: Justice: Sparks: E.E. Kershaw: D. Nicholas: Seldin: Inter-organ communication is a vital process to maintain physiologic homeostasis, and its dysregulation contributes many human diseases. Given that circulating bioactive factors are stable in serum, occur naturally, easily assayed from blood, they present obvious focal molecules for therapeutic intervention biomarker...
Abstract Disclosure: M. Zhou: None. I. Tamburini: C. Van: J. Molendijk: L.M. Velez: Nguyen: R. Yeo: Filho: A.L. Hevener: Justice: Sparks: E.E. Kershaw: D. Nicholas: Seldin: Inter-organ communication is a vital process to maintain physiologic homeostasis, and its dysregulation contributes many human diseases. Given that circulating bioactive factors are stable in serum, occur naturally, easily assayed from blood, they present obvious focal molecules for therapeutic intervention biomarker...
Abstract Objective Examine the association of ectopic adipose tissue (AT) with skeletal muscle (SM) mitochondrial bioenergetics in older adults. Methods Cross-sectional data from 829 adults ≥70 years was used. Total abdominal, subcutaneous, and visceral AT; thigh fat infiltration (MFI) quantified by MRI. SM energetics were characterized using vivo 31 P-MRS (ATP max ) ex high-resolution respirometry (maximal oxidative phosphorylation (OXPHOS)). ActivPal used to measure PA (step count). Linear...
Our group previously demonstrated that sarcoma cell lines were insensitive to epidermal growth factor receptor (EGFR) inhibitor gefitinib monotherapy. PENAO, an anti-tumour metabolic compound created in our laboratory, is currently clinical trials. Considering the positive regulation of tumour energy production by both EGFR signalling and metabolism pathways, this study aimed investigate effect mechanisms combination therapy using PENAO vitro vivo. monotherapy reduced proliferation 12 lines....
Abstract Aims/Hypothesis Insulin resistance and blunted mitochondrial capacity in skeletal muscle are often synonymous; however, this association remains controversial. The aim of study was to perform an in-depth multi-factorial comparison between individuals who were lean active (Active), with obesity (Obese) Obesity, insulin type 2 diabetes (T2D). Methods Skeletal biopsies obtained from the Vastus Lateralis (Active- n = 9), (Obese- 9) T2D (T2D- =22) cross-sectional design. Mitochondrial...
Inter-organ communication is a vital process to maintain physiologic homeostasis, and its dysregulation contributes many human diseases. Beginning with the discovery of insulin over century ago, characterization molecules responsible for signal between tissues has required careful elegant experimentation where these observations have been integral deciphering physiology disease. Given that circulating bioactive factors are stable in serum, occur naturally, easily assayed from blood, they...
Abstract/Introduction Inter-organ communication is a vital process to maintain physiologic homeostasis, and its dysregulation contributes many human diseases. Beginning with the discovery of insulin over century ago, characterization molecules responsible for signal between tissues has required careful elegant experimentation where these observations have been integral deciphering physiology disease. Given that circulating bioactive factors are stable in serum, occur naturally, easily...
Inter-organ communication is a vital process to maintain physiologic homeostasis, and its dysregulation contributes many human diseases. Beginning with the discovery of insulin over century ago, characterization molecules responsible for signal between tissues has required careful elegant experimentation where these observations have been integral deciphering physiology disease. Given that circulating bioactive factors are stable in serum, occur naturally, easily assayed from blood, they...