James Sanford
A5037518581- Ionosphere and magnetosphere dynamics
- Geophysics and Gravity Measurements
- Methane Hydrates and Related Phenomena
- Solar and Space Plasma Dynamics
- Adipose Tissue and Metabolism
- Dermatology and Skin Diseases
- Muscle metabolism and nutrition
- Acne and Rosacea Treatments and Effects
- Exercise and Physiological Responses
- Advanced Proteomics Techniques and Applications
- Skin Protection and Aging
- Antimicrobial Peptides and Activities
- Astrophysics and Cosmic Phenomena
- Advanced Biosensing Techniques and Applications
- Fibroblast Growth Factor Research
- Genetics and Physical Performance
- Adipokines, Inflammation, and Metabolic Diseases
- Asthma and respiratory diseases
- Cardiovascular and exercise physiology
- Advanced biosensing and bioanalysis techniques
- Dermatologic Treatments and Research
- Psoriasis: Treatment and Pathogenesis
- Hair Growth and Disorders
- melanin and skin pigmentation
- Yersinia bacterium, plague, ectoparasites research
Pacific Northwest National Laboratory
2012-2025
Colorado State University
2023
Wesleyan University
2021
University of California, San Diego
2012-2020
ORCID
2020
Kidderminster Hospital and Treatment Centre
1997
Quorum sensing peptides produced by commensal skin staphylococci protect against injury and inflammation induced Staphylococcus aureus toxins.
Regular exercise promotes whole-body health and prevents disease, but the underlying molecular mechanisms are incompletely understood
Microbial short-chain fatty acids inhibit HDAC activity and contribute to keratinocyte-triggered skin inflammation.
The regulation of cutaneous inflammatory processes is essential for the human skin to maintain homeostasis in presence dense communities resident microbes that normally populate this organ. Forming hair follicle-associated sebaceous gland, sebocytes are specialized lipid-producing cells can release mediators. Cytokine and chemokine expression by pilosebaceous epithelial (i.e., follicular keratinocytes) has been proposed contribute common disease acne vulgaris. underlying mechanisms drive...
Netherton syndrome (NS) is a monogenic skin disease resulting from loss of function lymphoepithelial Kazal-type-related protease inhibitor (LEKTI-1). In this study we examine if bacteria residing on the are influenced by LEKTI-1 and interaction between human gene resident contributes to disease. Shotgun sequencing microbiome demonstrates that lesional NS subjects dominated Staphylococcus aureus (S. aureus) epidermidis epidermidis). Isolates either species able induce inflammation barrier...
Abstract Subcutaneous white adipose tissue (scWAT) is a dynamic storage and secretory organ that regulates systemic homeostasis, yet the impact of endurance exercise training (ExT) sex on its molecular landscape not fully established. Utilizing an integrative multi-omics approach, leveraging data generated by Molecular Transducers Physical Activity Consortium (MoTrPAC), we show profound sexual dimorphism in scWAT sedentary rats response this to ExT. Specifically, females displays -omic...
Regular exercise has many physical and brain health benefits, yet the molecular mechanisms mediating effects across tissues remain poorly understood. Here we analyzed 400 high-quality DNA methylation, ATAC-seq, RNA-seq datasets from eight control endurance exercise-trained (EET) rats. Integration of baseline mapped gene location dependence epigenetic features identified differing regulatory landscapes in each tissue. The transcriptional responses to 8 weeks EET showed little overlap...
The potential for commensal microorganisms indigenous to a host (the ‘microbiome’ or ‘microbiota’) alter infection outcome by influencing host-pathogen interplay is largely unknown. We used multi-omics “systems” approach, incorporating proteomics, metabolomics, glycomics, and metagenomics, explore the molecular between murine host, pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), gut during intestinal with S. Typhimurium. find proteomic evidence that thrives within infected...
While regular physical activity is a cornerstone of health, wellness, and vitality, the impact endurance exercise training on molecular signaling within across tissues remains to be delineated. The Molecular Transducers Physical Activity Consortium (MoTrPAC) was established characterize networks underlying adaptive response exercise. Here, we describe studies undertaken by Preclinical Animal Sites Studies component MoTrPAC, in which sought develop implement standardized protocol large cohort...
In this study we evaluated the role of hyaluronan (HA) in reactive adipogenesis, a local expansion preadipocytes that provides host defense by release antimicrobial peptides. We observed HA accumulated during maturation adipocytes vitro and was associated with increased expression preadipocyte factor 1, zinc finger protein 423, early B cell 1. Although is normally abundant extracellular matrix, further increase staining occurred mice at sites adipogenesis following injury colon dextran...
Mitochondria are key regulators of metabolism and ATP supply in skeletal muscle, while circadian rhythms influence many physiological processes. However, whether mitochondrial function is intrinsically regulated a manner mouse muscle inadequately understood. Accordingly, we measured post-absorptive transcript abundance markers biogenesis, dynamics, (extensor digitorum longus [EDL], soleus, gastrocnemius), protein electron transport chain complexes (EDL soleus), enzymatic activity SDH...
Age-related changes in adipose tissue impact chronic medical diseases and mobility disability but mechanism remains poorly understood. The goal of this study is to define methods for phenotyping unique characteristics from older adults. Older adults enrolled Study Muscle, Mobility Aging selected the ancillary (SOMMA-AT; N=210, 52.38% women, 76.12±4.37 years) were assessed regional adiposity by whole-body magnetic resonance (AMRA) underwent a needle-aspiration biopsy abdominal subcutaneous...
White adipose tissue (WAT) plays a significant role in whole-body energy homeostasis, and its excess typifies obesity. In addition to WAT quantity, perturbations the basic cellular processes of (i.e. quality) are also associated with obesity metabolic disease. Exercise training alleviates obesity; however, underlying molecular mechanisms that drive these adaptations not well described. Abdominal subcutaneous biopsies were collected after an acute bout exercise (1 day after) at baseline...
Genome sequencing continues to be a rapidly evolving technology, yet most downstream aspects of genome annotation pipelines remain relatively stable or are even being abandoned. The process is now performed almost exclusively in an automated fashion balance the large number sequences generated. One possible way reducing errors inherent computational annotations apply data from omics measurements (i.e. transcriptional and proteomic) un-annotated with proteogenomic-based approach. Here,...
Isobaric labeling via tandem mass tag (TMT) reagents enables sample multiplexing prior to LC–MS/MS, facilitating high-throughput large-scale quantitative proteomics. Consistent and efficient reactions are essential achieve robust quantification; therefore, embedded in our clinical proteomic protocol is a quality control (QC) that contains small aliquot from each within TMT set, referred as "Mixing QC." This Mixing QC the detection of issues by LC–MS/MS before combining full samples allow for...
Abstract Regular exercise promotes whole-body health and prevents disease, yet the underlying molecular mechanisms throughout a whole organism are incompletely understood. Here, Molecular Transducers of Physical Activity Consortium (MoTrPAC) profiled temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome, immunome in blood, plasma, 18 solid tissues Rattus norvegicus over 8 weeks endurance training. The resulting data compendium...
Abstract Transcription factors (TFs) play a key role in regulating gene expression and responses to stimuli. We conducted an integrated analysis of chromatin accessibility, DNA methylation, RNA across eight rat tissues following endurance exercise training (EET) map epigenomic changes transcriptional determine TFs involved. uncovered tissue-specific TF motif enrichment all omic layers, differentially accessible regions (DARs), methylated (DMRs), expressed genes (DEGs). discovered distinct...
Physical activity, including structured exercise, is associated with favorable health-related chronic disease outcomes. Although there evidence of various molecular pathways that affect these responses, a comprehensive map responses to exercise has not been developed. The Molecular Transducers Activity Consortium (MoTrPAC) multicenter study designed isolate the effects training on mechanisms underlying health benefits and physical activity. MoTrPAC contains both preclinical human component....