A5068159926- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Plant and animal studies
- Muscle metabolism and nutrition
- Ecology and Vegetation Dynamics Studies
- Muscle activation and electromyography studies
- Shoulder Injury and Treatment
- Insect-Plant Interactions and Control
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Metabolism, Diabetes, and Cancer
- Adipokines, Inflammation, and Metabolic Diseases
- Sports injuries and prevention
- Skin and Cellular Biology Research
- Cardiovascular Disease and Adiposity
- Biological Control of Invasive Species
- Exercise and Physiological Responses
- Cardiomyopathy and Myosin Studies
- Diet and metabolism studies
- Botany, Ecology, and Taxonomy Studies
- Forest Insect Ecology and Management
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Lower Extremity Biomechanics and Pathologies
- Sirtuins and Resveratrol in Medicine
Washington University in St. Louis
2016-2025
American Physical Therapy Association
2023-2024
University of Wisconsin–Milwaukee
2007-2020
Université d'Avignon et des Pays de Vaucluse
2020
University of California, San Diego
2010-2015
United States Department of Veterans Affairs
2014
La Jolla Bioengineering Institute
2009-2011
Williams & Associates
2011
Allergan (Ireland)
2011
My25
2011
Muscle infiltration with adipose tissue (IMAT) is common and associated loss of skeletal muscle strength physical function across a diverse set pathologies. Whether the association between IMAT weakness causative or simply correlative remains an open question that needs to be addressed effectively guide strengthening interventions in people increased IMAT. In present studies, we demonstrate deposition causes decreased using mouse models. These findings indicate novel therapeutic target for...
The protein deacetylase, sirtuin 1 (SIRT1), is a proposed master regulator of exercise-induced mitochondrial biogenesis in skeletal muscle, primarily via its ability to deacetylate and activate peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α). To investigate regulation by SIRT1 vivo, we generated mice lacking deacetylase activity muscle (mKO). We hypothesized that deacetylation PGC-1α sedentary after endurance exercise would be impaired mKO mice. Skeletal contractile...
Osteoarthritis (OA), the leading cause of pain and disability worldwide, disproportionally affects individuals with obesity. The mechanisms by which obesity leads to onset progression OA are unclear due complex interactions among metabolic, biomechanical, inflammatory factors that accompany increased adiposity. We used a murine preclinical model lipodystrophy (LD) examine direct contribution adipose tissue OA. Knee joints LD mice were protected from spontaneous or posttraumatic OA, on either...
Abstract Endothelial cell (EC) CD36 controls tissue fatty acid (FA) uptake. Here we examine how ECs transfer FAs. FA interaction with apical membrane induces Src phosphorylation of caveolin-1 tyrosine-14 (Cav-1Y14) and ceramide generation in caveolae. Ensuing fission caveolae yields vesicles containing FAs, that are secreted basolaterally as small (80–100 nm) exosome-like extracellular (sEVs). We visualize transwells EC FAs sEVs to underlying myotubes. In mice EC-expression the exosome...
The giant protein obscurin is thought to link the sarcomere with sarcoplasmic reticulum (SR). N-terminus of interacts M-band proteins titin and myomesin, whereas C-terminus mediates interactions ankyrin proteins. Here, we investigate importance for SR architecture organization. Lack in cross-striated muscles leads changes longitudinal disruption small ankyrin-1.5 (sAnk1.5) expression localization. Changes knockout mice are also associated alterations several or SR-associated proteins, such...
Maintenance of skeletal muscle is beneficial in obesity and Type 2 diabetes. Mechanical stimulation can regulate differentiation, growth metabolism; however, the molecular mechanosensor remains unknown. Here, we show that SWELL1 ( Lrrc8a ) functionally encodes a swell-activated anion channel regulates PI3K-AKT, ERK1/2, mTOR signaling, myoblast fusion, cellular oxygen consumption, glycolysis cells. LRRC8A over-expression KO myotubes boosts PI3K-AKT-mTOR signaling to supra-normal levels fully...
Abstract Adipose tissue secretes numerous cytokines (termed ‘adipokines’) that have known or hypothesized actions on skeletal muscle. The majority of adipokines been implicated in the pathological link between excess adipose and muscle insulin resistance, but approximately half also documented vitro effects myogenesis and/or hypertrophy. This complexity suggests a potential dual role for regulation mass homeostasis development pathology. In this study, we used lipodystrophic ‘fat‐free’ mice...
Plant responses to herbivore damage vary enormously, but the causes of this variation are not well understood. We examined how both insect feeding style and soil fertility influence severity impacts, by measuring effects a leaf—chewing beetle (Trirhabda sp.), xylem sap—feeding spittlebug (Philaenus spumarius), phloem aphid (Uroleucon caligatum) on growth reproduction goldenrod (Solidago altissima). Our goals were determine (1) whether these three insects differentially affect plant fitness,...
Many plants support both leaf— and sap—feeding insects, yet comparative studies of plant responses to different types damage are rare. I compared the impacts a leaf—chewing beetle (Trirhabda sp.), xylem spittlebug (Philaenus spumarius), phloem aphid (Uroleucon caligatum) on growth goldenrod (Solidago altissima). My goals were (1) measure insect size as function herbivore loads, (2) determine mechanisms by which feeding affects growth, using techniques analysis. Plants grown outdoors in large...
Mitochondrial dysfunction has been implicated in the pathogenesis of type 2 diabetes. Identifying novel regulators mitochondrial bioenergetics will broaden our understanding regulatory checkpoints that coordinate complex metabolic pathways. We previously showed Nur77, an orphan nuclear receptor NR4A family, regulates expression genes linked to glucose utilization. Here we demonstrate Nur77 skeletal muscle also enhances function. generated MCK-Nur77 transgenic mice express wild-type...
The material properties of passive skeletal muscle are critical to proper function and frequently a target for therapeutic interventional strategies. Investigations into the viscoelasticity have primarily focused on characterizing elastic behavior, largely neglecting viscous component. However, viscosity is sizeable contributor stress extensibility during stretch thus there need characterization as well components viscoelasticity. Single mouse fibers were subjected incremental relaxation...
A fundamental requirement of cells is their ability to transduce and interpret mechanical environment. This contributes regulation growth, differentiation adaptation in many cell types. The intermediate filament (IF) system not only provides passive structural support the cell, but recent evidence points IF involvement active biological processes such as signaling, mechanotransduction gene regulation. However, mechanisms that underlie these are well known. Skeletal muscle provide a...
Skeletal muscle is a dynamic composite of proteins that responds to both internal and external cues facilitate adaptation. In cases disease or altered use, these messages can be distorted resulting in myopathic conditions such as fibrosis. this work, we describe mild progressive fibrotic adaptation skeletal lacking the cytoskeletal intermediate filament protein desmin. Muscles desmin become progressively stiffer, accumulate increased collagen, increase expression genes involved extracellular...
Differences in passive muscle mechanical properties between amphibians and mammals have led to differing hypotheses on the functional role of titin skeletal muscle. Early studies frog clearly demonstrated intracellular load bearing by titin, but more recent structural biological mice shown that may serve other functions. Here, we present biomechanical isolated mouse fibers, fiber bundles compare relative importance versus extracellular these species. Mouse exhibited increased modulus...
Diabetic patients have a high risk of developing skeletal diseases accompanied by diabetic peripheral neuropathy (DPN). In this study, we isolated the role DPN in disease with global and conditional knockout models sterile-α TIR-motif-containing protein-1 (Sarm1). SARM1, an NADase highly expressed nervous system, regulates axon degeneration upon range insults, including DPN. Global Sarm1 prevented DPN, but not disease, male mice type 1 diabetes (T1D). Female wild also developed bone without...
Mitochondria are key regulators of metabolism and ATP supply in skeletal muscle, while circadian rhythms influence many physiological processes. However, whether mitochondrial function is intrinsically regulated a manner mouse muscle inadequately understood. Accordingly, we measured post-absorptive transcript abundance markers biogenesis, dynamics, (extensor digitorum longus [EDL], soleus, gastrocnemius), protein electron transport chain complexes (EDL soleus), enzymatic activity SDH...
The timed contraction and relaxation of myofibers in tissues such as the heart skeletal muscle occurs via tightly regulated movement calcium ions into out sarcoplasmic reticulum (SR). In muscle, this phenomenon enables humans to exercise, perform day-to-day tasks, breathe. Sarcolipin, a small regulatory protein, prevents from entering SR by binding inhibiting SERCA, contributing myofiber contraction. Disruptions sarcolipin expression are implicated pathophysiology obesity musculoskeletal...