A5023318732- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Genomics and Chromatin Dynamics
- Genetic factors in colorectal cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Lung Cancer Treatments and Mutations
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Medical Imaging and Pathology Studies
- Lung Cancer Diagnosis and Treatment
- Colorectal Cancer Screening and Detection
- Advanced MRI Techniques and Applications
- Glioma Diagnosis and Treatment
- Head and Neck Cancer Studies
- Viral-associated cancers and disorders
- Systemic Sclerosis and Related Diseases
- Medical Imaging Techniques and Applications
- Neonatal Respiratory Health Research
- Veterinary Oncology Research
- Chromatin Remodeling and Cancer
- Advanced Nanomaterials in Catalysis
- Molecular Biology Techniques and Applications
Volition (Belgium)
2014-2025
Université Libre de Bruxelles
2012
Institut de génétique et de biologie moléculaire et cellulaire
2004-2010
Centre National de la Recherche Scientifique
2010
Collège de France
2010
Inserm
2010
Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie
1992-1995
Technical University of Munich
1992
Interstitial lung disease and associated fibrosis occur in a proportion of individuals who have recovered from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection through unknown mechanisms. We studied with 2019 (COVID-19) after recovery illness. Individuals evidence interstitial changes at 3 to 6 months had an up-regulated neutrophil-associated immune signature including increased chemokines, proteases, markers neutrophil extracellular traps that were detectable the...
To improve the prognosis of patients with pancreatic cancer, new biomarkers are required for earlier, pre-symptomatic diagnosis. Epigenetic mutations take place at earliest stages tumorigenesis and therefore offer approaches detecting diagnosing disease. Nucleosomes repeating subunits DNA histone proteins that constitute human chromatin. Because their release into circulation, intact nucleosome levels in serum or plasma can serve as diagnostic disease biomarkers, elevated have been reported...
Colonoscopy is currently widely accepted as the gold standard for detection of colorectal cancer (CRC) providing up to 95% pre-cancerous lesions during procedure. However, certain limitations exist in most countries including cost and access Moreover, colonoscopy an invasive technique with risk inherent endoscopic For this reason, alternative screening tests, particular, fecal occult blood-based have been adopted frontline screening. Limited compliance approaches has prompted research on...
The transcriptional intermediary factor 1β (TIF1β) is a corepressor for KRAB-domain-containing zinc finger proteins and believed to play essential roles in cell physiology by regulating chromatin organization at specific loci through association with remodeling histone-modifying activities recruitment of heterochromatin protein 1 (HP1) proteins. In this study, we have engineered modified embryonal carcinoma F9 line ( TIF1 β HP1box/- ) expressing mutated TIF1β (TIF1β HP1box unable interact...
The severity of coronavirus disease 2019 (COVID-19) varies significantly with cases spanning from asymptomatic to lethal a subset individuals developing Severe Acute Respiratory Syndrome (SARS) and death respiratory failure. To determine whether global nucleosome citrullinated levels were elevated in COVID-19 patients, we tested two independent cohorts positive patients quantitative immunoassays found that nucleosomes highly plasma severe course the relative healthy controls both histone 3.1...
Abstract Recent advances in basic research have unveiled several strategies for improving the sensitivity and specificity of cell-free DNA (cfDNA) based assays, which is a prerequisite broadening its clinical use. Included among these leveraging knowledge both biogenesis physico-chemical properties cfDNA towards identification better disease-defining features optimization methods. While good progress has been made on this front, much biology remains uncharted. Here, we correlated serial...
Alteration of epigenetic modifications plays an important role in human cancer. Notably, the dysregulation histone post-translational (PTMs) has been associated with several cancers including colorectal cancer (CRC). However, signature PTMs on circulating nucleosomes is still not well described. We have developed a fast and robust enrichment method to isolate from plasma for further downstream proteomic analysis. This enabled us quantify global alterations 9 CRC patients healthy donors....
Background: Neutrophil extracellular traps’ (NETs’) formation is a mechanism of defense that neutrophils deploy as an alternative to phagocytosis, constrain the spread microorganisms. Aim: The aim was evaluate biomarkers NETs’ in patient cohort admitted intensive care unit (ICU) due infection. Methods: Forty-six septic shock patients, 22 critical COVID-19 patients and 48 matched control subjects were recruited. Intact nucleosomes containing histone 3.1 (Nu.H3.1), or citrullinated H3R8...
Background/Objectives: Accurate non-invasive tests to improve early detection and diagnosis of lung cancer are urgently needed. However, no regulatory-approved blood available for this purpose. We aimed pulmonary nodule classification identify malignant nodules in a high-prevalence patient group. Methods: This study involved 806 participants with undiagnosed larger than 5 mm, focusing on assessing nucleosome levels histone modifications (H3.1 H3K27Me3) circulating blood. Nodules were...
Neutrophil extracellular traps (NETs) are chromatin-based web-like structures released by activated neutrophils in response to infectious agents. Overproduction or insufficient clearance of NETs contributes dysfunction immune and disease pathogenesis, underlying the importance early detection monitoring NET levels clinical samples. While existing methods for quantification face limitations, there is a pressing need reliable, sensitive, clinically applicable assay. Since consist long strains...
Purpose: Plasma cell-free nucleosome (cf-nucleosome) measurements are used in veterinary, but not human medicine, to identify asymptomatic subjects at high risk of cancer. Uniquely among plasma sandwich immunoassay tests, cf-nucleosomes nucleoproteins with a complex electrochemistry. Formaldehyde crosslinking is common the investigation cellular by chromatin immunoprecipitation. cf-nucleosome similarly involves isolation from We investigated crosslinked cancer samples. Experimental Design:...
The aim was to evaluate serum levels of circulating cell-free nucleosomes (ccfn) containing a variety epigenetic signals including 5-methylcytosine DNA, histone modifications H3K9Me3, H3K9Ac, H3S10PO4, H3K36Me3, H4K20Me3, H4PanAc and pH2AX, nucleosome variant H2AZ adducts with HMGB1 EZH2 as well ccfn per se, in addition develop predictor models based on the above mentioned carcinoembryonic antigen (CEA), early detection colorectal cancer (CRC). Blood-samples were collected from 4,105...
Idiopathic pulmonary fibrosis (IPF) is a progressive, fatal lung disorder of unknown origin with highly variable and unpredictable clinical course. Polymorphisms environmentally induced epigenetic variations seem to determine individual susceptibility the development fibrosis.We have studied circulating epitopes on cell-free nucleosomes (cfnucleosomes) in 50 IPF patients. We compared untreated (n = 23) receiving antifibrotic therapy 27) healthy subjects (HS) 27). analyzed serum levels five...
Histone demethylation has important roles in regulating gene expression and forms part of the epigenetic memory system that regulates cell fate identity by still poorly understood mechanisms.Here, we examined role histone demethylase Kdm3a during differentiation, showing is essential for differentiation into parietal endoderm-like (PE) cells F9 mouse embryonal carcinoma model.We identified a number target genes regulated endoderm differentiation; among most dysregulated were three...
Abstract During cell death, nucleosomes, the basic structural unit of chromatin, are released into blood stream and elevated levels have been found in plasma patients with solid cancers. In this study, we demonstrate an increase free circulating H3.1-nucleosomes samples from hematological malignancy, non-Hodgkin lymphoma (NHL), relative to healthy donors. As histone post-translational modifications (PTMs) nucleosomes described as potential biomarkers various cancers, investigated epigenetic...
Nucleosomes consist of small fragments DNA wrapped around a histone octamer core. Diseases such as cancer or inflammation lead to cell death, which causes fragmentation and release nucleosomes into the blood. The Nu.Q™ technology measures circulating nucleosome levels exploits different compositions derived in blood detect identify even at early stages. objectives this study are optimal sample type for H3.1 assay determine if it can accurately healthy canines well those with cancer.Blood...
Abstract NETosis is an innate immune response occurring after infection or inflammation: activated neutrophils expel decondensed DNA in complex with histones into the extracellular environment a controlled manner. It activates coagulation and fuels risk of thrombosis. Human pregnancy associated mild proinflammatory state characterized by circulatory neutrophil activation which further increased complicated pregnancies, placenta-mediated complications being thrombotic risk. This aberrant...
Aim: To evaluate pre-analytical variables of circulating cell-free nucleosomes containing 5-methylcytosine DNA (5mC) or histone modification H3K9Me3 (H3K9Me3).Materials and methods: Six studies were designed to assess the possible influence variables. Study 1: stasis contamination with white-cells platelets. 2: within-day variations. 3: day-to-day variation. 4: temperature during handling storage, neoplastic disease. 5: colonoscopy. 6: surgical trauma. 5mC measurements performed using...
Abstract Background There is an unmet need for noninvasive markers specific kidney transplant rejection. Such a marker may eventually overcome the biopsy. In this pilot study, potential of circulating cell-free nucleosomes (CCFN) to serve as biomarker rejection was evaluated. Methods Forty de novo recipients were prospectively followed part randomized, controlled clinical trial. Total CCFN (H3) and with histone modifications H3K36me3 H3 citrulline measured in patients at four fixed time...
Abstract Introduction Nucleosomes consist of small fragments DNA wrapped around a histone octamer core. Diseases such as cancer or inflammation lead to cell death, which causes fragmentation and release nucleosomes into the blood. The Nu.Q™ technology measures circulating nucleosome levels exploits different compositions derived in blood detect identify even at early stages. objectives this study are optimal sample type for H3.1 assay determine if it can accurately healthy canines well those...