A5083521662- Genetic factors in colorectal cancer
- Protease and Inhibitor Mechanisms
- Colorectal Cancer Treatments and Studies
- Colorectal Cancer Surgical Treatments
- Peptidase Inhibition and Analysis
- Colorectal Cancer Screening and Detection
- Cancer Genomics and Diagnostics
- Blood transfusion and management
- Gastric Cancer Management and Outcomes
- Cell Adhesion Molecules Research
- Complement system in diseases
- Cardiac, Anesthesia and Surgical Outcomes
- Mast cells and histamine
- Angiogenesis and VEGF in Cancer
- Immune Cell Function and Interaction
- Platelet Disorders and Treatments
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Molecular Biology Techniques and Applications
- Cancer, Lipids, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Studies on Chitinases and Chitosanases
- Enhanced Recovery After Surgery
- Radiomics and Machine Learning in Medical Imaging
Hvidovre Hospital
2013-2022
University of Copenhagen
2013-2022
Copenhagen University Hospital
2010-2021
Voss Kommune
2011-2018
Odense University Hospital
1977-2016
University of Southern Denmark
2016
Frederick National Laboratory for Cancer Research
2007-2014
National Cancer Institute
2007-2014
Helse Førde
2014
University of Bergen
2012-2014
Updated National Academy of Clinical Biochemistry (NACB) Laboratory Medicine Practice Guidelines for the use tumor markers in clinic have been developed.Published reports relevant to 5 cancer sites--testicular, prostate, colorectal, breast, and ovarian--were critically reviewed.For testicular cancer, alpha-fetoprotein, human chorionic gonadotropin, lactate dehydrogenase are recommended diagnosis/case finding, staging, prognosis determination, recurrence detection, therapy monitoring....
To evaluate the potential for diagnosing colorectal cancer (CRC) from faecal metagenomes.We performed metagenome-wide association studies on samples 74 patients with CRC and 54 controls China, validated results in 16 24 Denmark. We further biomarkers two published cohorts France Austria. Finally, we employed targeted quantitative PCR (qPCR) assays to diagnostic of selected an independent Chinese cohort 47 109 controls.Besides confirming known associations Fusobacterium nucleatum...
Noninvasive liquid biopsy analysis of circulating tumor DNA permits direct detection early-stage cancers.
<h3>Objective</h3> To develop an affordable and robust pipeline for selection of patient-specific somatic structural variants (SSVs) being informative about radicality the primary resection, response to adjuvant therapy, incipient recurrence treatment performed in relation diagnosis recurrence. <h3>Design</h3> We have established efficient procedures identification SSVs by next-generation sequencing subsequent quantification 3–6 plasma. The consequence intratumour heterogeneity on our...
Abstract Background Bowel dysfunction after sphincter-preserving surgery for rectal cancer is a common complication, with the potential to affect quality of life (QoL) strongly. The aim this study was examine extent bowel and impact on health-related QoL curative resection cancer. Methods assessed using European Organization Research Treatment Cancer QLQ-C30 questionnaire, function validated including recently developed low anterior syndrome (LARS) score. Assessments were carried out at time...
Abstract Non-invasive approaches for cell-free DNA (cfDNA) assessment provide an opportunity cancer detection and intervention. Here, we use a machine learning model detecting tumor-derived cfDNA through genome-wide analyses of fragmentation in prospective study 365 individuals at risk lung cancer. We validate the using independent cohort 385 non-cancer 46 patients. Combining features, clinical factors, CEA levels, followed by CT imaging, detected 94% patients with across stages subtypes,...
Abstract Purpose: We investigated whether detection of ctDNA after resection colorectal cancer identifies the patients with highest risk relapse and, furthermore, longitudinal analysis allows early and informs about response to intervention. Experimental Design: In this cohort study, we used massively parallel sequencing identify somatic mutations these as markers detect minimal residual disease monitor changes in tumor burden during a 3-year follow-up period. Results: A total 45 371 plasma...
Approximately 25% of all patients with stage II colorectal cancer will experience recurrent disease and subsequently die within 5 years. MicroRNA-21 (miR-21) is upregulated in several types has been associated survival colon cancer. In the present study we developed a robust situ hybridization assay using high-affinity Locked Nucleic Acid (LNA) probes that specifically detect miR-21 formalin-fixed paraffin embedded (FFPE) tissue samples. The expression was analyzed by on 130 67 rectal...
<h3>Abstract</h3> <b>Objective:</b> To evaluate the influence of preoperative abstinence on postoperative outcome in alcohol misusers with no symptoms who were drinking equivalent at least 60 g ethanol/day. <b>Design:</b> Randomised controlled trial. <b>Setting:</b> Copenhagen, Denmark. <b>Subjects:</b> 42 alcoholic patients without liver disease admitted for elective colorectal surgery. <b>Interventions:</b> Withdrawal from consumption 1month before operation (disulfiram controlled)...
Overall peritumoural inflammatory cell infiltration is a prognostic variable in solid tumours, but the survival-related impact of individual types within infiltrate has still not been fully evaluated and compared with conventional disease classification. In present study, value white counts colorectal cancer was assessed. Intra-operative tumour tissue samples from 584 patients undergoing elective surgery for were included. None received pre- or post-operative adjuvant chemotherapy. Tissue...
Summary We have developed a system for the simultaneous labelling of two specific chromosomal sites using different fluorescent ParB/ parS systems. Using this, we demonstrate that chromosome arms are spatially arranged in newborn cells such markers on left arm lie one half cell and right opposite half. This is achieved by reorganizing nucleoids pre‐division relative to quarters. The spatial reorganization ensures replication forks remain halves during replication. orientation reorganized not...
Summary We have followed the fate of 14 different loci around Escherichia coli chromosome in living cells at slow growth rate using a highly efficient labelling system and automated measurements. Loci are segregated as they replicated, but with marked delay. Most markers segregate smooth temporal progression from origin to terminus. Thus, overall pattern is one continuous segregation during replication not consistent recently published models invoking extensive sister cohesion by...
Abstract Background Acute high-risk abdominal (AHA) surgery carries a very high risk of morbidity and mortality represents massive healthcare burden. The aim the present study was to evaluate effect standardized multidisciplinary perioperative protocol in patients undergoing AHA surgery. Methods prospective single-centre controlled consecutive surgery, defined as major pathology requiring emergency laparotomy or laparoscopy including reoperations after elective gastrointestinal Consecutive...
At all but the slowest growth rates, Escherichia coli cell cycles overlap, and its nucleoid is segregated to daughter cells as a forked DNA circle with replication ongoing—a state fundamentally different from eukaryotes. We have solved chromosome organization, structural dynamics, segregation of this constantly replicating chromosome. It locally condensed form branched donut, compressed so that least replicated spans center newest extends toward poles. Three narrow zones at quarters contain...
Abstract Background Early detection plays an essential role to reduce colorectal cancer (CRC) mortality. While current screening methods suffer from poor compliance, liquid biopsy-based strategies for is rapidly gaining promise. Here, we describe the development of TriMeth, a minimal-invasive blood-based test early-stage cancer. The based on assessment three tumour-specific DNA methylation markers in circulating cell-free DNA. Results A thorough multi-step biomarker discovery study profiles...
Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. These contain biomolecules, including proteins microRNAs (miRNAs). Both circulating EVs miRNAs have received much attention as biomarker candidates for non‐invasive diagnostics. Here we describe a sensitive analytical method isolation subsequent miRNA profiling of epithelial‐derived from blood samples patients with colorectal cancer (CRC). The were isolated by...
YKL-40 is a mammalian member of the chitinase protein family. Although function unknown, pattern its expression suggests in remodelling or degradation extracellular matrix. High serum has been found patients with recurrent breast cancer and related to short survival. In present study we analysed preoperative sera from colorectal evaluated relation Serum was determined by RIA 603 patients. Survival after operation registered, median follow-up time 61 months. Three hundred forty died. Sixteen...