A5100694091- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Rice Cultivation and Yield Improvement
- MicroRNA in disease regulation
- Powdery Mildew Fungal Diseases
- Extracellular vesicles in disease
- Bacterial Genetics and Biotechnology
- Plant Pathogens and Fungal Diseases
- Bacteriophages and microbial interactions
- Plant Pathogens and Resistance
- RNA modifications and cancer
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Arsenic contamination and mitigation
- Fungal Biology and Applications
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Yeasts and Rust Fungi Studies
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Chinese history and philosophy
- Chromosomal and Genetic Variations
- Research in Cotton Cultivation
- Advanced biosensing and bioanalysis techniques
- Heavy Metal Exposure and Toxicity
Ruijin Hospital
2019-2025
Shanghai Jiao Tong University
2018-2025
Oklahoma State University Oklahoma City
2012-2024
Washington State University
2024
Yunnan Agricultural University
2024
Oklahoma State University
2009-2024
Qinghai University
2022-2024
Henan Normal University
2011-2022
Fudan University
2022
China Medical University
2013-2021
Abstract Extracellular vesicles (EVs) have shown great potential for treating various diseases. Translating EVs‐based therapy from bench to bedside remains challenging due inefficient delivery of EVs the injured area and lack techniques visualize entire targeting process. Here we developed a dopamine surface functionalization platform that facilitates easy simultaneous conjugation peptide multi‐mode imaging probes EVs. Utilizing this concurrently modified M2 microglia‐derived (M2‐EVs) with...
When a bactericidal antibiotic is added to growing bacterial culture, the great majority of population killed but small number metabolically quiescent bacteria called persisters survive treatment. The mechanism this persistence poorly understood. In Escherichia coli, we identified new gene, phoU, whose inactivation leads generalized higher susceptibility than that parent strain diverse range antibiotics, including ampicillin, norfloxacin, and gentamicin, stresses, such as starvation, acid...
Expression profiling of the 5′ ends uncapped mRNAs (“degradome” sequencing) can be used to empirically catalog microRNA (miRNA) targets, probe patterns miRNA hairpin processing, examine mRNA decay, and analyze accumulation endogenous short interfering RNA (siRNA) precursors. We sequenced analyzed degradome moss Physcomitrella patens , an important model system for functional genomic analyses in plant evolution. A total 52 target 27 different families were identified. Many targets both more...
Summary We have followed the fate of 14 different loci around Escherichia coli chromosome in living cells at slow growth rate using a highly efficient labelling system and automated measurements. Loci are segregated as they replicated, but with marked delay. Most markers segregate smooth temporal progression from origin to terminus. Thus, overall pattern is one continuous segregation during replication not consistent recently published models invoking extensive sister cohesion by...
Background Methylenetetrahydrofolate reductase (MTHFR) C677T, A1298C and methionine synthase (MTRR) A66G polymorphisms are important genetic determinants for homocysteine (Hcy) levels, associated with several disorders. These heterogeneously distributed worldwide. Our objective was to explore the geographical distributions of these in China. Methodologies 15357 healthy adults were recruited from 10 regions. Buccal samples collected genomic DNA isolated. Genotyping performed using fluorogenic...
Rationale: Glial scars present a major obstacle for neuronal regeneration after stroke. Thus, approaches to promote their degradation and inhibit formation are beneficial stroke recovery. The interaction of microglia astrocytes is known be involved in glial scar stroke; however, how affect remains unclear. Methods: Mice were treated daily with M2 microglial small extracellular vesicles through tail intravenous injections from day 1 7 middle cerebral artery occlusion. scar, infarct volume,...
Central nervous system (CNS) injuries, including stroke, traumatic brain injury, and spinal cord are essential causes of death long-term disability difficult to cure, mainly due the limited neuron regeneration glial scar formation. Herein, we apply extracellular vesicles (EVs) secreted by M2 microglia improve differentiation neural stem cells (NSCs) at injured site, simultaneously modify them with vascular targeting peptide (DA7R) cell recruiting factor (SDF-1) on their surface via...
Recently, post-transcriptional regulation of mRNA mediated by N6-methyladenosine (m6A) has been found to have profound effects on transcriptome during plant responses various abiotic stresses. However, whether this RNA modification can affect an oxidative stress response in plants not studied. To assess the role m6A modifications copper-induced responses, m6A-IP-seq was performed Arabidopsis seedlings exposed high levels copper sulfate. This analysis revealed large-scale shifts transcripts...
In plants, microRNAs (miRNAs) regulate their mRNA targets by precisely guiding cleavages between the 10th and 11th nucleotides in complementary regions. High-throughput sequencing-based methods, such as PARE or degradome profiling coupled with a computational analysis of sequencing data, have recently been developed for identifying miRNA on genome-wide scale. The existing algorithms limit number mismatches its strictly do not allow mismatch G:U Wobble pair at position 10 11. However,...
Global warming predictions indicate that temperatures will increase by another 2-6°C the end of this century. High temperature is a major abiotic stress limiting plant growth and productivity in many areas world. Switchgrass (Panicum virgatum L.) model herbaceous bioenergy crop, due to its rapid rate, reliable biomass yield, minimal requirements water nutrients, adaptability grow on marginal lands widespread distribution throughout North America. The effect high switchgrass physiology, cell...
Abstract Chronic arsenic exposure has been associated with various toxic effects, especially to the organs of liver and kidney. As a plant polyphenol, curcumin is most vital bioactive ingredient turmeric wide range pharmacological activities. In present study, we investigated potential roles against arsenic‐induced kidney dysfunctions in mice. Curcumin treatment (200 mg/kg) not only decreased deposition kidney, but also relieved hepatic nephritic biochemical indexes (Glutamic oxaloacetic...
Protecting the integrity of blood-brain barrier (BBB) is crucial for maintaining brain homeostasis after ischemic stroke. Previous studies showed that M2 microglial extracellular vesicles (EVs) played a neuroprotective role in cerebral ischemia. However, EVs BBB unclear. Therefore, we explored mechanisms regulating integrity. To identify EVs, used nanoparticle tracking analysis, transmission electron microscopy, and western blot analysis. Adult male ICR mice were subjected to 90-min middle...
Escherichia coli chromosome replication forks are tethered to the cell centre. Two opposing models describe how chromosomes segregate. In extrusion-capture model, newly replicated DNA is fed bi-directionally from toward poles, forming new in each half. Starting with origins, chromosomal regions segregate away their sisters progressively as they replicated. The termini last. sister cohesion produces that paired along much of length. origins and most other remain until late cycle, all...
We studied the segregation of replication terminus Escherichia coli chromosome by time-lapse and still photomicroscopy. The replicated termini lie together at cell centre. They rapidly segregate away from each other immediately before division. At fast growth rate, copies move progressively quickly toward centres new-born cells. slow usually remain near inner pole migrate to centre in middle cycle. A domain about 160kb, roughly centred on dif recombination site, segregated as a unit...
Soil salinity is one of the primary causes yield decline in rice. Pokkali (Pok) a highly salt-tolerant landrace, whereas IR29 salt-sensitive but widely cultivated genotype. Comparative analysis these genotypes may offer better understanding tolerance mechanisms Although most stress-responsive genes are regulated at transcriptional level, many cases, changes level not always accompanied with protein abundance, which suggests that transcriptome needs to be studied conjunction proteome link...
Drought stress is a serious problem worldwide that reduces crop productivity. The laser has been shown to play positive physiological role in enhancing plant seedlings tolerance various abiotic stresses. However, little information available about the molecular mechanism of He-Ne irradiation induced changes for wheat adapting drought conditions. Here, we performed large-scale transcriptome sequencing determine roles pretreated under stress. There were 98.822 transcripts identified, and,...
MiRNAs and phasiRNAs are negative regulators of gene expression. These small RNAs have been extensively studied in plant model species but only 10 mature microRNAs present miRBase version 21, the most used miRNA database, no identified for legume Phaseolus vulgaris. Thanks to recent availability first common bean genome, degradome data RNA libraries, we able here a catalog this organism and, particularly, suggest new protagonists symbiotic nodulation events.We set 185 miRNAs, including 121...