A5007996044- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Breast Cancer Treatment Studies
- HER2/EGFR in Cancer Research
- Lung Cancer Treatments and Mutations
- Prostate Cancer Diagnosis and Treatment
- Genetic factors in colorectal cancer
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- Colorectal Cancer Treatments and Studies
- Advanced Breast Cancer Therapies
- Molecular Biology Techniques and Applications
- Protease and Inhibitor Mechanisms
- Cancer Risks and Factors
- Medical Imaging and Pathology Studies
- Vitamin D Research Studies
- Chemokine receptors and signaling
- Bone health and treatments
- Lung Cancer Research Studies
- Cancer Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Advanced Biosensing Techniques and Applications
- Cancer Treatment and Pharmacology
- BRCA gene mutations in cancer
- Peptidase Inhibition and Analysis
Clinique du Millénaire
2015-2025
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2019-2024
Institut de Génétique Humaine
2024
Languedoc Mutualité
2022
Institut d'Imagerie Biomédicale
2018-2022
Université de Toulouse
2021
Institut de Recherche en Cancérologie de Montpellier
2004-2020
Montpellier GenomiX
2020
Institut Curie
2019
Centre Jean Perrin
2019
<h3>Objective</h3> To develop an affordable and robust pipeline for selection of patient-specific somatic structural variants (SSVs) being informative about radicality the primary resection, response to adjuvant therapy, incipient recurrence treatment performed in relation diagnosis recurrence. <h3>Design</h3> We have established efficient procedures identification SSVs by next-generation sequencing subsequent quantification 3–6 plasma. The consequence intratumour heterogeneity on our...
We used a novel method based on allele-specific quantitative polymerase chain reaction (Intplex) for the analysis of circulating cell.free DNA (ccfDNA) to compare total ccfDNA and KRAS- or BRAF-mutated concentrations in blood samples from mice xenografted with human SW620 colorectal cancer (CRC) cell line patients CRC. Intplex enables single-copy detection variant alleles down sensitivity ≥0.005 mutant wild-type ratio. The proportion allele corresponding percentage tumor-derived was elevated...
Abstract Background Circulating tumour cells (CTCs), circulating DNA (ctDNA), and extracellular vesicles (EVs) are minimally invasive liquid biopsy biomarkers. This study investigated whether they predict prognosis, alone or in combination, heterogenous unbiased non-small cell lung cancer (NSCLC) patients. Methods Plasma samples of 54 advanced NSCLC patients from a prospective clinical trial. CtDNA mutations were identified using the UltraSEEK™ Lung Panel (MassARRAY® technology). PD-L1...
We examined the feasibility of using a panel autoantibodies to multiple tumor-associated proteins as method for early detection breast cancer and, more particularly, carcinoma in situ (CIS).PPIA, PRDX2, and FKBP52 were identified early-stage autoantigens by proteomic approaches. The seroreactivity antibodies consisting these three antigens two previously described autoantigens, HSP60 MUC1, was tested on 235 samples (60 from primary patients, 82 CIS 93 healthy controls) with use specific...
Circulating tumor cells (CTCs) are important clinical indicators for prognosis and treatment efficacy. However, CTC investigation is hampered by their low number, making the establishment of permanent lines very challenging. We derived characterized nine using blood samples from a patient with metastatic colorectal cancer collected before after chemotherapy targeted therapy, during progression. These cell displayed an intermediate epithelial/mesenchymal phenotype, stem-cell like...
A link between neuroendocrine cell differentiation and resistance to androgen deprivation has been observed in prostate cancer, suggesting the possible efficacy of specific treatments. We assessed toxicity a chemotherapy regimen combining docetaxel cisplatin men with independent prostatic adenocarcinoma circulating markers.A total 41 patients were treated combination 75 mg/m(2) every 3 weeks for maximum 6 cycles. The primary study end point was response rate, defined as decrease neuron...
Human epidermal growth factor receptor 2 (HER2)-amplified breast cancers represent a tumor subtype with chromosome 17q rearrangements that lead to frequent gene amplifications. The aim of this study was quantify the amplification genes located on and analyze relations between pattern amplifications patients' characteristics survival. Patients HER2-positive tumors (HER2 score 3+ by immunohistochemistry or positive for HER2 fluorescence in situ hybridization (FISH)) (n = 86) HER2-negative 40)...
Until now, FISH has been the gold standard technique to identify HER2 amplification status in ambiguous cases of breast cancer. Alternative techniques have developed increase capacities investigating status. The aims this multicenter study a large series cancer patients were prospectively compare level performance CISH, SISH, and qPCR alternative on paraffin-embedded core biopsies with "gold FISH" for evaluation This was performed 840 scored by immunohistochemistry (IHC): 0=317 (38%), 1+=183...
In luminal androgen receptor (AR) tumours, FOXA1 may direct AR to sites occupied by ER in thus stimulating proliferation.AR and expression were evaluated immunohistochemistry 333 non-metastatic triple-negative breast cancers (TNBC). Positivity threshold was set at ≥ 1% staining. Lymphocytic infiltration, PD-L1expression, PIK3CA mutations, PTEN defects BRCA1 promoter methylation assessed.AR + /FOXA1 tumours (42.4%) more frequently: found older patients, lobular, of lower nuclear grade, with...
Abstract Chronic inflammation may play a role in prostate cancer carcinogenesis. In that context, our objective was to investigate the of nonsteroidal anti‐inflammatory drugs ( NSAID s) risk based on EPICAP data. is population‐based case–control study carried out 2012–2013 département Hérault, France) enrolled 819 men aged less than 75 years old newly diagnosed for and 879 controls frequency matched cases age. Face face interviews gathered information several potential factors including s...
Prostate cancer remains the most frequent among men, representing a significant health burden. Despite its high morbidity and mortality rates, etiology of prostate relatively unknown, with only non-modifiable established risk factors. Chronic inflammation has emerged as potential factor in carcinogenesis. We investigated role kidney gallbladder stones cancer. used data from EPICAP, population-based case-control study. A total 819 diagnosed cases 879 controls were face-to-face interviewed...
KRAS mutation has been unambiguously identified as a marker of resistance to cetuximab-based treatment in metastatic colorectal cancer (mCRC) patients. However, most studies analysis have performed using homogenously archived CRC specimens, and that compare freshly frozen specimens formalin-fixed paraffin-embedded (FFPE) are lacking. The aim the present study was evaluate impact tissue preservation on determination mutational status. A series 131 mCRC fresh-frozen tissues were first analyzed...
Poly(adenosine diphosphate–ribose) polymerase 1 (PARP-1) and the balance between BRCA1 53BP1 play a key role in DNA repair cell stress response. PARP inhibitors show promising clinical activity metastatic triple negative (TN) or BRCA-mutated breast cancer. However, comprehensive analysis of PARP-1 activity, promoter methylation expression tumours without known mutation has not yet been carried out. We investigated cytosolic protein levels 155 surgical tumour samples from patients familial...