A5061548362- Diet and metabolism studies
- Nutrition and Health in Aging
- Clinical Nutrition and Gastroenterology
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Lipoproteins and Cardiovascular Health
- Traumatic Brain Injury and Neurovascular Disturbances
- Cholesterol and Lipid Metabolism
- Sepsis Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Hormonal Regulation and Hypertension
- Electrolyte and hormonal disorders
- Diet, Metabolism, and Disease
- Anesthesia and Neurotoxicity Research
- Adipose Tissue and Metabolism
- Education, Psychology, and Social Research
- Adipokines, Inflammation, and Metabolic Diseases
- Pharmacology and Obesity Treatment
- Gastric Cancer Management and Outcomes
- Hemodynamic Monitoring and Therapy
- Plant Physiology and Cultivation Studies
- Liver Disease Diagnosis and Treatment
- Oral health in cancer treatment
- Fatty Acid Research and Health
- Blood transfusion and management
- Natural Antidiabetic Agents Studies
University Hospital Hradec Králové
2016-2025
Charles University
2013-2024
Brno University of Technology
2004-2024
University of Hradec Králové
2017-2024
Research Institute of Inorganic Chemistry
2019
University of Pardubice
2002
A new and relatively simple method for purification of betulin from birch bark extract was developed in this study. Its five steps are based on the differential solubility components various solvents their crystallization and/or precipitation, affinity Ca(OH)2 ethanol, some impurities silica gel chloroform. In addition, all used can be simply recycled. Betulin more than 99% purity prepared by with minimal costs. Various observations including betulin, changes crystals during heating, attempt...
Abstract Background: Omentin-1 is an anti-inflammatory adipokine produced preferentially by visceral adipose tissue. Plasma levels of omentin-1 are decreased in obesity and other insulin-resistant states. Insulin resistance contributes to the changes cholesterol synthesis absorption as well. The aim this study was characterise plasma obese patients with diabetes mellitus type 1 during weight reduction, elucidate relationship between metabolism omentin-1. Methods: were measured diabetics ( n...
Background The ischemia-reperfusion injury (IRI) is unavoidable in vascular surgery. Damage to the microcirculation and endothelial glycocalyx might set up a shock with loss of circulatory coherence organ failure. Sulodexide may help protect alleviate injury. Methods Twenty female piglets underwent surgery 30-min-long suprarenal aortic clamp, followed by two hours reperfusion. Ten received sulodexide before 10 normal saline. Blood urine samples were taken at baseline 20-min intervals until...
Background: Sulodexide is a glycosaminoglycan-based drug prescribed to patients with angiopathy. We performed pilot study investigate whether sulodexide positively modulates the endothelial glycocalyx (EG) layer and microcirculation in porcine model of EG enzymatic damage. The sugar-based lining that involved physiology capillary wall pathogenesis many diseases. Methods: damage was induced eight piglets by hyaluronidase III heparanase I given intravenously. Four animals received 600 IU...
Coronavirus disease (COVID-19) associated endotheliopathy and microvascular dysfunction are of concern.The objective the present single-center observational pilot study was to compare endothelial glycocalyx (EG) damage in patients with severe COVID-19 (COVID-19 group) bacterial pneumonia septic shock (non-COVID group).Biomarkers EG (syndecan-1), cells (EC) (thrombomodulin), activation (P-selectin) were measured blood on three consecutive days from admission intensive care unit (ICU). The...
Hydrogen is a potent antioxidant agent that can easily be administered by inhalation. The aim of the study was to evaluate whether hydrogen protects endothelial glycocalyx layer after successful cardiopulmonary resuscitation (CPR).Fourteen anesthetized pigs underwent CPR induced ventricular fibrillation. During and return spontaneous circulation, 2% gas seven (hydrogen group) constituted control group. Biochemistry sublingual microcirculation were assessed at baseline, during CPR, 15th,...
The endothelial glycocalyx (EG) lining the endoluminal surface of capillaries has been proposed as a key component microcirculation and major player in microvascular pathology. Recent advances understanding its physiological role clinical significance have made upon development methods allowing EG assessment medicine. Laboratory can assess amount damage by measuring levels degradation products (e.g. syndecan-1, heparan sulphate hyaluronan sulphate), mostly plasma, however, their turnover...
Adhesions are the most common cause of long-term morbidity after abdominal surgery and often various forms intestinal passage disorders ranging from partial obstruction to complete, life-threatening obstruction. The aim present study was evaluate protective effect intraperitoneally administered lipid emulsions on formation adhesions in larger animal model, as lubricating phospholipids mechanical barrier component combined with anti-inflammatory fish oil.Thirty-one female domestic pigs were...
Aims: We are currently witnessing changes in views on the evaluation of serum proteins. A decrease may signal not only malnutrition. It also be an indicator simultaneously occurring inflammatory disease. Prealbumin, due to its short half-life, is a suitable protein-energy balance, but levels show, as with other proteins, case inflammation too. The present study aimed determine prealbumin values hospitalized geriatric patients and how they affected by Methods: In 101 aged over 80 years,...
The present study seeks to identify the nutritional risk factors involved in development of neuropathies induced by chemotherapeutic treatments. Unlike gastrointestinal or hematological adverse effects chemotherapy there is no protective treatment strategy for polyneuropathy. aim this was find possible deficiencies factors, which can be used supplementation future prevention chemotherapy-induced neuropathy development. We analyzed 70 patients undergoing paclitaxel and evaluated peripheral...
Introduction: Our study aim was to assess how the macronutrient intake during total parenteral nutrition (TPN) modulates plasma free fatty acids (FFAs) levels and individual in critically ill patients. Method: Adult patients aged 18–80, admitted intensive care unit (ICU), who were indicated for TPN, with an expected duration of more than three days, included study. Isoenergetic isonitrogenous TPN solutions given a major non-protein energy source, which glucose (group G) or lipid emulsions...
Combination of platinum derivatives with paclitaxel is currently the standard front line regimen for patients epithelial ovarian carcinoma, and represents also an active in metastatic breast or unknown primary carcinomas. Measurement intestinal permeability one potential methods noninvasive laboratory assessment gastrointestinal mucositis induced by chemotherapy, but little known about treated platinum. Intestinal was assessed 36 cancer paclitaxel/platinum combination measuring, using...
Colorectal cancer is a clinical condition whose treatment often involves intestinal resection. Such frequently results in two major gastrointestinal complications after surgery: anastomotic leakage and prolonged ileus. Anastomotic serious complication which, more than not, diagnosed late; to date, C-reactive protein the only available diagnostic marker. A monocentric, prospective, open case-control study was performed patients<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
Obesity in T1DM patients is associated with the components of metabolic syndrome. The influence controlled fasting and low calorie diet (LCD) on insulin sensitivity glucose metabolism was studied 14 obese type 1 diabetes mellitus (T1DM) (42.6+/-9.4 years, BMI 32.4+/-2.1 kg m(-2)). Insulin measured using a hyperinsulinemic-euglycemic clamp before fasting, immediately after 7 days 21 LCD. Glucose oxidation non-oxidative disposal were during by indirect calorimetry. In control group 13...
Somatic cell count (SCC) is an indicator of mammary gland health state.Lactose (L) can be reduced with mastitis and SCC increase.Mammary essential factor milk quality.Monitoring important for prevention treatment secretion disorders.The goal this work was to analyse the relationship between L in various biological species.7 sets individual bulk samples (MSs) were analysed (n = 479, 345, 80, 90 102) content. 3 cow (C) 1 set goat (G), sheep (S) human (H) MSs.The relations C used as...