Oral squamous cell carcinoma (OSCC) is a highly aggressive malignancy with poor prognosis, mainly due to the presence of cancer stem cells (CSCs), small subpopulation that contribute therapy resistance and tumor progression. The principal objective this study was investigate role miRNA-21 in maintenance stemness possibility altering it. CD44 antigen used as marker for CSC isolation from oral cultures. CD44+ CD44- populations were sorted via magnetic separation. inhibition performed...

Abstract Taking advantage of the flexibility apatite structure, nano- and micro-particles hydroxyapatite (HAp) were doped with different combinations rare earth ions (RE 3+ = Gd, Eu, Yb, Tm) to achieve a synergy among their magnetic optical properties enable application in preventive medicine, particularly diagnostics based on multimodal imaging. All powders synthesized through hydrothermal processing at T ≤ 200 °C. An X-ray powder diffraction analysis showed that all crystallized P 6 3 / m...

Hydroxyapatite (HAp) has been the main protagonist in quest for an ideal biomaterial regenerative medicine over last half a century. To control its properties, this material commonly doped with chemical elements other than natural stoichiometric constituents: Ca, O, P, and H. Here, we report on first analysis of biological response to germanium-doped hydroxyapatite (Ge-HAp). Cytotoxicity, osteogenic differentiation induction, colony formation potential were measured dental pulp stem cells,...

Oral cancer ranks in the top 10 most prevalent malignancies worldwide. It is an aggressive tumor with frequent relapses and metastases relatively modest survival rates that do not improve spite of constantly evolving treatment modalities. Cancer stem cells are a subpopulation considered to be responsible only for initiation but also its behavior. Many efforts directed at targeting those specifically. A class small molecules, inhibitors BET proteins (iBET), emerging as novel anticancer tool....

Background/Objectives: Cold atmospheric plasma (CAP) has shown a strong anticancer effect on variety of tumors, presenting new approach for the effective treatment oral squamous cell carcinoma (OSCC), one most prevalent malignant neoplasms with high mortality rate. Here, we aimed to comprehensively investigate antitumor potential two approaches CAP both two-dimensional and three-dimensional OSCC line models, as well analyze whether enhances sensitivity chemotherapy. Methods: An in-house...

Background/Objectives: A novel 3D-printed, bioresorbable bone graft, made of nanohydroxyapatite (nHAP) covered by poly(lactide-co-glycolide) (PLGA), showed strongly expressed osteoinductive properties in our previous investigations. The current study examines its application dual regeneration and cartilage combining with nHAP gel obtained enrichment hydroxyethyl cellulose, sodium hyaluronate, chondroitin sulfate. Methods: In the vitro part study, mitochondral activity osteogenic chondrogenic...

Background: Oral cancer is an aggressive malignancy with modest survival rates. It also causes disfigurement following surgical removal of the tumor, thus highlighting need for new treatment and tissue repair modalities. Carbon-based nanomaterials have emerged as promising tools in both anticancer regenerative therapies. Objectives: We aimed to synthesize a carbon-based nanomaterial (CBN) test its antineoplastic effects, well potential capacity. Materials Methods: A carbon nanomaterial,...

Background/Objectives: A novel 3D-printed, bioresorbable bone graft, made of nanohydroxyapatite (nHAP) covered by poly(lactide-co-glycolide) (PLGA), showed strongly expressed osteoinductive properties in our previous investigations. The current study examines its application the dual regeneration and cartilage combining with nHAP gel obtained enrichment hydroxyethyl cellulose, sodium hyaluronate, chondroitin sulfate. Methods: In vitro part study, mitochondrial activity osteogenic...

Antidepressant use is on the rise, while problem of depression a population wide level not being tackled. One hypothesis why this may be happening effect adverse effects and withdrawal symptoms antidepressants, as well pathologization normal sadness. In study we did partial watchful waiting using (1) psychosocial advice; (2) depathologization; (3) shared decision making. The comprised 83 consecutive non-suicidal out-patients with depressive symptoms. first among three main findings our was...

Collagen membranes are routinely used in oral surgery for bone regeneration. Despite their numerous advantages, such as stimulating growth, bacterial contamination still remains one of the disadvantages membrane use. Thus, we assessed biocompatibility and osteogenic antibacterial properties a collagen (OsteoBiol) modified with chitosan (CHI) hydroxyapatite nanoparticles (HApNPs). Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR FT-IR), X-ray powder diffraction (XRD),...

Dentures and epitheses are mostly made from poly(methyl methacrylate) (PMMA), which does not show antimicrobial properties. They present reservoirs of microorganisms grown in biofilms. The aim this study is to prepare a PMMA enriched with gold nanoparticles (AuNPs)-PMMA/AuNPs the examination its physical, mechanical AuNPS were synthetized HAuCl4 using ultrasonic spray pyrolysis method lyophilization. PMMA/AuNP samples compared samples. Density was measured by pycnometer. Microhardness...

Improving immune status in neonates is crucial to health and production. Gut active carbohydrates (GAC) have been associated with increasing immunoglobin levels immonucompetence development mammals. The objective of the following studies was evaluate whether GAC (mannan-oligosaccharides) applied orally progeny immediately parturition, improved blood plasma immunoglobulin (Ig) type G concentrations piglets calves. Three trials were conducted comparing control groups those receiving orally....

Despite improvements in oral squamous cell carcinoma (OSCC) management, survival rates remain relatively low and novel anti-neoplastic agents are needed. Bromodomain extra-terminal (BET) inhibitors proved to be promising for cancer treatment. We investigated the effects of three BET (JQ1, IBET-151, IBET-762) on SCC-25 line primary culture.Cell viability was evaluated by MTT. Protein levels MCM5 cleaved-PARP were estimated Western blot. Clonogenic migratory abilities determined colony forming...

Introduction: There is a known connection between periodontitis and atherosclerosis the presence of periopathogens in blood vessels. However, changes oral microflora related to aging process its possible effects on atherosclerosis, have yet be analyzed. The aim this study was assess temporal frequency periodontal bacteria subgingival plaque atherosclerotic vessels patients with atherosclerosis.
 Methodology:The included 100 periodontitis, divided into two groups, below over 60 years...

(1) Treatment failure of oral squamous cell carcinoma (OSCC) is generally due to the development therapeutic resistance caused by existence cancer stem cells (CSCs), a small subpopulation with marked self-renewal and differentiation capacity. Micro RNAs, notably miRNA-21, appear play an important role in OSCC carcinogenesis. Our objectives were explore multipotency CSCs estimating their capacity assessing effects on stemness, apoptosis, several miRNAs’ expression. (2) A commercially...

Undiagnosed and untreated oral precancerous lesions often progress into malignancies. Photodynamic therapy (PDT) might be a minimally invasive alternative to conventional treatments. 5-aminolevulinic acid (5-ALA) is one of the most commonly used photosensitizers in PDT, it effective on many cancer types. However, its hydrophilic characteristic limits cell membrane crossing. In present study, effect newly formulated gel containing 5% 5-ALA combination with red light (ALAD-PDT) premalignant...

Abstract Background Understanding the pathogenesis of basal cell carcinoma ( BCC ) and identifying cells responsible for propagation recurrence are crucial development new treatment strategies. The aim this study was to characterize isolated from its margin. Methods Primary cultures were established 10 s, their respective close resection margins (3 mm) control tissues. Stem markers analysis carried out by real‐time PCR and/or flow cytometry. Spheroid formation MTT assays also performed....

Background/Aim: Current approaches in therapy of head and neck cancers are surgery, radiotherapy chemotherapy. However, recurrence, development multidrug resistance, side effects, high costs significant problems which point to the need for more efficient less toxic drugs interventions. Material Methods: Eight essential oils obtained from Thymus serpyllum, Mentha piperita, Juniperus communis, Rosmarinus officinalis, Melissa Achillea millefolium, Zingiber officinale, Helichrysum arenarium were...