- Nematode management and characterization studies
- Horticultural and Viticultural Research
- Forest Insect Ecology and Management
- Insect-Plant Interactions and Control
- Forest ecology and management
- Plant Surface Properties and Treatments
- Genetics and Plant Breeding
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Forest Biomass Utilization and Management
- Plant Physiology and Cultivation Studies
- Genomics and Phylogenetic Studies
- Genetic diversity and population structure
- Genetic Mapping and Diversity in Plants and Animals
- Plant and animal studies
- Genetic and phenotypic traits in livestock
- Plant and Fungal Interactions Research
- Optimal Experimental Design Methods
- Nuts composition and effects
- Parasite Biology and Host Interactions
- Plant pathogens and resistance mechanisms
- Turfgrass Adaptation and Management
- Marine Biology and Ecology Research
- Forest Management and Policy
- Agricultural and Food Sciences
Instituto Nacional de Investigação Agrária e Veterinária
2012-2023
University of Lisbon
2020-2023
Abstract Cork oak ( Quercus suber ) is native to southwest Europe and northwest Africa where it plays a crucial environmental economical role. To tackle the cork production industrial challenges, advanced research imperative but dependent on availability of sequenced genome. address this, we produced first draft version We followed de novo assembly strategy based high-throughput sequence data, which generated genome comprising 23,347 scaffolds 953.3 Mb in size. A total 79,752 genes 83,814...
Abstract Purpose of Review Recognizing that in the context global change, tree genetic diversity represents a crucial resource for future forest adaptation, we review and highlight major genetics research achievements past decades biodiversity-rich countries Mediterranean region. For this, conducted bibliometric analysis scientific literature spanning thirty years (1991–2020). Putting together representative regionwide expertise our co-authorship, propose perspectives next decade. Recent...
Cork oak (Quercus suber) is one of the rare trees with ability to produce cork, a material widely used make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms cork formation are still poorly understood, in great part due difficulty studying species long life-cycle for which there scarce molecular/genomic information. forests ecological importance represent major economic social resource Southern Europe Northern Africa. However, global...
Pine wilt disease (PWD), caused by the plant–parasitic nematode Bursaphelenchus xylophilus , has become a severe environmental problem in Iberian Peninsula with devastating effects Pinus pinaster forests. Despite high levels of this species' susceptibility, previous studies reported heritable resistance P. trees. Understanding basis can be extreme relevance for future programs aiming at reducing impact on In study, we highlighted mechanisms possibly involved to PWD, comparing transcriptional...
The pinewood nematode (PWN) is the causal agent of pine wilt disease, a pathology that affects conifer forests, mainly Pinus spp. PWN infection can induce expression phytohormone-related genes; however, changes at early phytohormone level have not yet been explored. Phytohormones are low-abundance metabolites, and thus, difficult to quantify. Moreover, most methodologies focus on Arabidopsis or crop species. This work aimed validate fast (run time 6.6 min) liquid chromatography-triple...
Summary The pinewood nematode ( PWN ), B ursaphelenchus xylophilus , is a serious quarantine pest first detected in P ortugal and E urope 1999. It the causal agent of pine wilt disease PWD ). A resistance breeding programme has been initiated to contribute control evolution disease. Five hundred four adult maritime pine, inus pinaster trees were phenotypically selected as candidate for this from an area affected by . To identify tolerance nematode, monitored monthly. Over course 1 year, 57...
The pinewood nematode (PWN) Bursaphelenchus xylophilus is the causal agent of pine wilt disease (PWD) and represents one major threats to conifer forests. detection PWN in Portugal, associated with Pinus pinaster, increased concern its spread European Despite susceptibility PWD, genetic variability found among P. pinaster populations has been heritable PWD resistance. Understanding mechanisms underlying tree resistance constitutes a valuable resource for breeding programs toward more...
Background and Aims The evaluation of genotype-by-environment interaction the genotypic correlations between important economic traits are two relevant issues methodology grapevine selection that remain insufficiently explored. aim this study is to provide methodological tools to: (i) assess (ii) evaluate genetic their practical impacts on selection. Methods Results A multi-environment analysis for each trait multi-trait analyses were applied correlation pairs traits, respectively. presence...
Background and Aims The development of an efficient clonal selection process requires the study genotype-by-environment (G × E) interaction. This work aims to evaluate variability G E interaction among genotypes identify less sensitive ones. Methods Results approach involves fitting mixed models yield data taking into account correlation induced by repeated measurements same plot over years. A measure for comparative evaluation is proposed [Interaction Sensitivity (IS)], based on variance...
Pinewood nematode (PWN, Bursaphelenchus xylophilus) is the causal agent of pine wilt disease (PWD), which severely affects Pinus pinaster stands in southwestern Europe. Despite high susceptibility P. pinaster, individuals selected half-sib families have shown genetic variability survival after PWN inoculation, indicating that breeding for resistance can be a valuable strategy to control PWD. In this work, RNA-seq data from susceptible and resistant plants inoculated with were used SNP...
Background and Aims. In ancient grapevine varieties, the experimental design of field trials is crucial to providing a reliable evaluation quantitative traits. The main purposes this study are demonstrate benefits resolvable row-column (RCD) for quantifying intravarietal variability performing polyclonal selection compare efficiency fully partially replicated designs implement latter preliminary analysis that variability. Methods Results. Linear mixed models were fitted yield data obtained...
The Pinewood nematode Bursaphelenchus xylophilus (PWN) was found to be responsible for maritime pine (Pinus pinaster) death the first time in Europe 1999, a Portuguese forest south of Lisbon. It is causal agent Pine Wilt Disease (PWD) and quarantine organism European Union with approximately €80 million spent from 1999 – 2009 attempting its eradication, which must also added direct losses tree mortality. During this period, important progress has been made understanding relationships between...
Abstract Cork oak ( Quercus suber L.) is an ecologically and economically important evergreen tree species native to the Mediterranean region widespread in southwest Europe northwest Africa. An improved genome assembly of cork using a combination Illumina PacBio sequencing presented this study. The assembled contains 2351 scaffolds longer than 1000 bp, accounting for 765.7 Mbp size, L90 755, N50 1.0 Mbp, with 40,131 annotated genes. repetitive sequences constitute 53.6% genome. chloroplast...
In 1933, Joaquim Vieira Natividade established an experimental field with oaks and related genera at Mata Nacional do Vimeiro, Portugal. This area includes the only Portuguese plots hybrid Quercus x hispanica ‘Lucombeana’ (Quercus cerris suber) seeds obtained from Cambridge Botanic Gardens.In 1962-1965, their offspring were included in this area. Those integrate ex situ forest genetic resources, representing a unique population for understanding structure, function evolution of genome...