A5063072269- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Chromosomal and Genetic Variations
- Plant and Fungal Interactions Research
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Plant biochemistry and biosynthesis
- Plant tissue culture and regeneration
- Plant Pathogens and Fungal Diseases
- Genetic diversity and population structure
- Plant Physiology and Cultivation Studies
- Wine Industry and Tourism
- Plant and animal studies
- RNA Research and Splicing
- HIV Research and Treatment
- Plant Surface Properties and Treatments
- Plant Virus Research Studies
- Allelopathy and phytotoxic interactions
- Molecular Biology Techniques and Applications
- Plant Taxonomy and Phylogenetics
- Seedling growth and survival studies
- Tree Root and Stability Studies
University of Lisbon
2012-2023
University of Minho
2019
University of Pannonia
2019
Agriculture and Food
2019
Instituto de Biologia Experimental e Tecnológica
1997-2009
Universidade Nova de Lisboa
2008
Centro de Genética Clínica
2007
Instituto Nacional de Investigação Agrária e Veterinária
2004
ABSTRACT The asymmetric shape of the Antirrhinum corolla is determined by genes acting differentially along dorsoventral axis flower. Genes so far identified determine asymmetry in dorsal regions. We describe a gene, divaricata, which contrast to previously acts ventral regions show analysis mutant combinations that divaricata gene negatively regulated cycloidea and dichotoma. In addition, we chromosomal duplications dosage-dependent manner, suggesting level its product critical for...
BackgroundPolyploidization is a major evolutionary process in plants where hybridization and chromosome doubling induce enormous genomic stress can generate genetic epigenetic modifications. However, proper evaluation of DNA sequence restructuring events the precise characterization sequences involved are still sparse.Methodology/Principal FindingsInter Retrotransposons Amplified Polymorphism (IRAP), Microsatellite (REMAP) Inter Simple Sequence Repeat (ISSR) largely confirmed absence any...
Wild plants of Vitis closely related to the cultivated grapevine (V. v. vinifera) are believed have been first domesticated 10,000 years BC around Caspian Sea. V. vinifera is hermaphrodite whereas sylvestris a dioecious species. Male flowers show reduced pistil without style or stigma and female present reflexed stamens with infertile pollen. produce perfect all functional structures. The mechanism for flower sex determination specification in still unknown. To understand which genes...
Abstract Background Quantitative real time polymerase chain reaction is becoming the primary tool for detecting mRNA and transcription data analysis as it shows to have advantages over other more commonly used techniques. Nevertheless, also presents a few shortcomings, with most import being need normalisation, usually reference gene. Therefore choice of gene(s) great importance correct analysis. Microarray data, when available, can be assistance choosing genes. Grapevine was submitted water...
Monoecious species provide a comprehensive system to study the developmental programs underlying establishment of female and male organs in unisexual flowers. However, molecular resources for most monoecious non-model are limited, hampering our ability mechanisms involved flower development these species. The objective this was identify differentially expressed genes during flowers Quercus suber, an economically important Mediterranean tree. Total RNA extracted from different stages Q. suber...
Cork oak (Quercus suber) is one of the rare trees with ability to produce cork, a material widely used make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms cork formation are still poorly understood, in great part due difficulty studying species long life-cycle for which there scarce molecular/genomic information. forests ecological importance represent major economic social resource Southern Europe Northern Africa. However, global...
Vitis vinifera is a hermaphrodite subspecies, while its ancestor, sylvestris, dioecious. We have identified two genes that together allow the discrimination between male, female and plants. The sex locus region on chromosome 2 was screened resulting in discovery of new gene, VviFSEX. same screening revealed another VviAPRT3, located region, be used as marker. Both are good candidates to involved flower differentiation grapevine. To assess their role specification, spatial temporal expression...
Predicted climate changes announce an increase of extreme environmental conditions including drought and excessive heat light in classical viticultural regions. Thus, understanding how grapevine responds to these different genotypes can adapt, is crucial for informed decisions on accurate actions. Global transcriptome analyses are useful this purpose as the response abiotic stresses involves interplay complex diverse cascades physiological, cellular molecular events. The main goal present...
The most discriminating characteristic between the cultivated Vitis vinifera subsp. and wild-form sylvestris is their sexual system. Flowers of cultivars are mainly hermaphroditic, whereas wild plants have female male individuals whose flowers follow a hermaphroditic pattern during early stages development later develop non-functional reproductive organs. In angiosperms, basic developmental system for floral organ identity explained by ABCDE model. This model postulates that regulatory gene...
Cork oak (Quercus suber) is an important Portuguese species, mainly due to the economic value of cork it produces. results from phellogen, a meristematic tissue, which can locally produce lenticels or have discontinuities, originating "defects": pores and nail inclusions that are detrimental industrial use. Epigenetic processes control plant development its deregulation lead altered phenotypes; therefore, study epigenetic players in phellogen understand emergence cork's defects. DNA...
The 35S ribosomal DNA (rDNA) units, repeated in tandem at one or more chromosomal loci, are separated by an intergenic spacer (IGS) containing functional elements involved the regulation of transcription downstream rRNA genes. In present work, we have compared IGS molecular organizations two divergent species Fagaceae, Fagus sylvatica and Quercus suber, aiming to comprehend evolution sequences within family. Self- cross-hybridization FISH was done on representative Fagaceae. length...
Vitis vinifera can be divided into two subspecies, V. subsp. vinifera, one of the most important agricultural crops in world, and its wild ancestor, sylvestris. Three flower types observed: hermaphrodite female (on some varieties) male or flowers It is assumed that different ancestor arose through specific floral patterns organ abortion. A considerable amount data about diversity sexual systems grapevines has been collected over past century. Several grapevine breeding studies led to...
Abstract We developed 20 polymorphic nuclear microsatellites from Pinus pinaster using genomic libraries enriched with repeated motifs. A total of 25 individuals five different populations were used to estimate genetic diversity parameters.
Transposable elements (TEs) make up a large part of eukaryotic genomes. Due to their repetitive nature and the fact that they harbour regulatory signals, TEs can be responsible for chromosomal rearrangements, movement gene sequences evolution regulation function. Retrotransposon ubiquity raises question about function in genomes most are transcriptionally inactive due rearrangements compromise activity. However, activity is currently considered have been one major processes genome evolution....
Widespread agricultural losses attributed to drought, often combined with high temperatures, frequently occur in the field, namely Mediterranean climate areas, where existing scenarios for change indicate an increase frequency of heat waves and severe drought events summer. Grapevine (Vitis vinifera L.) is most cultivated fruit species world valuable one a traditional species. Currently, viticulture must adjust impending changes that are already pushing vine-growers toward use ancient...
Abstract The first genome of Vitis vinifera (PN40024), published in 2007, boosted grapevine related studies. While this reference is a suitable tool for the overall studies field, it lacks ability to unveil changes accumulated during V. v. domestication. subspecies sylvestris preserves wild characteristics, making good material provide insights into difference reproductive strategy between both one characteristics that set them apart. flowers are hermaphrodite, mostly dioecious. In paper, we...
Oaks, chestnuts, and beeches are economically important species of the Fagaceae. To understand relationship between these members this family, a deep knowledge their genome composition organization is needed. In work, we have isolated characterized several AFLP fragments obtained from Quercus rotundifolia Lam. through homology searches in available databases. Genomic polymorphisms involving some sequences were evaluated two , one Castanea Fagus with specific primers. Comparative FISH...
The sweet chestnut tree (Castanea sativa Mill.) is one of the most significant Mediterranean species, being an important natural resource for wood and fruit industries. It a monoecious presenting unisexual male catkins bisexual catkins, with latter having distinct female flowers. Despite importance tree, little known regarding molecular mechanisms involved in determination sexual organ identity. Thus, study how different flowers C. develop fundamental to understand reproductive success this...