A5046207401- Pharmacogenetics and Drug Metabolism
- Drug Transport and Resistance Mechanisms
- Malaria Research and Control
- Biochemical Analysis and Sensing Techniques
- Computational Drug Discovery Methods
- Ion channel regulation and function
- Traditional and Medicinal Uses of Annonaceae
- Acute Lymphoblastic Leukemia research
- Mangiferin and Mango Extracts
- Ethnobotanical and Medicinal Plants Studies
- Eicosanoids and Hypertension Pharmacology
- Antibiotics Pharmacokinetics and Efficacy
- Ion Channels and Receptors
- BRCA gene mutations in cancer
- Statistical Methods in Clinical Trials
- Prenatal Screening and Diagnostics
- Glutathione Transferases and Polymorphisms
- Mosquito-borne diseases and control
- Cancer Genomics and Diagnostics
- Natural Compound Pharmacology Studies
- Diet and metabolism studies
- Gout, Hyperuricemia, Uric Acid
- Carcinogens and Genotoxicity Assessment
- Gastrointestinal Tumor Research and Treatment
- SARS-CoV-2 and COVID-19 Research
Obafemi Awolowo University
2015-2024
Mahidol Oxford Tropical Medicine Research Unit
2022
Mahidol University
2022
University of Oxford
2022
Guangdong University of Technology
2019-2020
University of Florida
2018
Columbus Oncology and Hematology Associates
2018
The varied disposition of the antimalarial quinine partly explains its poor tolerance and toxicity in humans.Using a population approach, healthy subjects patients with acute uncomplicated symptomatic malaria from Nigeria was re-examined view to providing population-specific attributes.Concentration versus time profiles over 48 hours individuals, 7 days malaria-infected patients, were stratified reflect: concentration data during first administration for infected after all available...
In the absence of drugs to treat or prevent COVID-19, drug repurposing can be a valuable strategy. Despite substantial number clinical trials, did not deliver on its promise. While success was observed with some repurposed (e.g., remdesivir, dexamethasone, tocilizumab, baricitinib), others failed show efficacy. One reason is lack clear translational processes based adequate preclinical profiling before evaluation. Combined limitations existing in vitro and vivo models, there need for...
CYP1A2 and CYP2A6 are polymorphic enzymes that metabolise several compounds of clinical importance. This study investigated the prevalent phenotypes these influence age sex on enzyme activity in a Nigerian population.Caffeine (110 mg) was administered to each 129 healthy, unrelated subjects (85 males 44 females) who were non-smokers. Urine voided within 7 h after caffeine administration collected for high performance liquid chromatographic assay (137X), 1,7-dimethyluric acid (17U)...
Thiopurine S-methyltransferase (TPMT) methylates clinically relevant thiopurine drugs most of which are noted for adverse reactions in certain users, largely due to polymorphisms the TPMT gene. This study investigated prevalence functionally alleles Nigerian population. One hundred eighty unrelated subjects consisting 123 males and 57 females from main ethnicities (44 Igbo, 101 Yoruba, 23 Hausa 12 other minor ethnic groups) were genotyped TPMT*2, *3B, *3C *4 using iPLEX genotyping assay...
Abstract Background: CYP1A2 and CYP2A6 are polymorphic drug-metabolising enzymes that also implicated in the activation of procarcinogens humans. Some their alleles haplotypes, often varied prevalence across populations, thought to influence activity despite known contribution environmental factors. This study assessed potential some genetic variants on metabolic phenotypes Nigerians. Methods: Genomic DNA was extracted from blood samples 100 healthy, unrelated subjects for whom had...
Malaria is a leading cause of morbidity and mortality in many developing tropical countries, particularly children pregnant women. With growing concerns about the development resistance to current antimalarial drugs, herbal alternatives may provide significant sustainable treatment options affected regions. This review aimed at providing an updated overview available data on Herbal Medicinal Products (HMPs) with reproducible evidence-based efficacies. In addition, it identified...
Imatinib has been successful in the management of chronic myeloid leukemia (CML) but some patients experience adverse reactions or develop resistance to its use. The roles polymorphisms genes encoding enzymes critical for biotransformation imatinib have previously examined. This study, hence, evaluated other unstudied functionally significant CYP1A2, CYP2C8, CYP2C9, and CYP3A5. Trough blood levels genotypes were determined 42 CML by an HPLC-UV technique a Sequenom iPLEX assay, respectively....
Purpose: To investigate the interaction between quinine and Garcinia kola using an in vitro adsorption study.Methods: In G. was conducted at 37 ± 0.1 °C. Adsorption of (2.5 - 40 μg/ml) to 2.5 % w/v suspension studied. Thereafter, desorption process investigated. The amount adsorbed desorbed quantified HPLC. A Freundlich isotherm constructed describe resulting data percentage desorbedwas determined from data.Results: An gave a constant (K) 52.66 μg/g, with slope 0.69 indicating high capacity...
Background: MAMA Decoction (MD) is prepared from the leaves of Mangifera indica, Alstonia boonei, Morinda lucida and Azadirachta indica. A co-administration MD with amodiaquine led to synergism in clearance malaria parasites a previous report. The pharmacokinetic basis for this observation was subject another study mice which found significant MD- induced increase exposure half-life desethylamodiaquine, major metabolite amodiaquine.Objective: This aimed at evaluating previously identified...
Abstract Amodiaquine (AQ) and pioglitazone (PGZ) are both metabolized by CYP2C8, an enzyme also inhibited PGZ. These drugs likely to be administered in instances of comorbidity malaria with type 2 diabetes. This study, hence, evaluated the possibility a drug interaction resulting from concurrent use drugs. A 3‐period crossover design 10 healthy subjects, that assessed disposition AQ PGZ alone when coadministered, was implemented administration single oral doses Whole‐blood samples collected...
This study assessed the activity of thiopurine S-methyltransferase (TPMT) in Nigerians with a view to providing data on susceptibility toxicity, and as well generate reference values for clinical use.TPMT activity, expressed amount 6MMP ng/mL after 1 h incubation at 37 °C per haemoglobin (U/g Hb), varied between 2.34 63.50 U/g Hb population. Poor metabolic phenotypes, characterised by an below 8.41 Hb, were observed 20% subjects. Intermediate metabolizers had 16.13 Hb. Fast very fast...
A simple and rapid method for simultaneous determination of amodiaquine pioglitazone in dried blood spots (DBS) was developed validated. Blood samples were spotted on protein saver cards a 4-mm punch extracted with methanol first later 1% acetic acid dichloromethane. The separation achieved C8 Zorbax Eclipse XDB analytical column (4 µm, 150 × 4.6 mm2 i.d.) at 27°C mobile phase methanol/0.2% (60:40) flow rate 0.8 mL/min detected 230 nm. linear over the range 2–80 ng/mL 10–1500 correlation...
1. Wadelius M, Sörlin K, Wallerman O, et al. Warfarin sensitivity related to CYP2C9, CYP3A5, ABCB1 (MDR1) and other factors. Pharmacogenomics J. 2004;4(1):40–48. https://doi.org/10.1038/sj.tpj.... CrossRef Google Scholar
Isosteviol is a synthetic derivative of steviol glycosides with promising pharmacological properties and might find future use as cardioprotective agent. A simple LC-MS/MS technique was developed validated for the bioanalysis isosteviol in plasma erythrocytes. This method subsequently utilized vitro assessment isosteviol's partitioning into blood compartments humans rats. Fresh samples from healthy Wistar rats were equilibrated 1, 10, 30 µM at 37 °C shaking dry-bath. The levels erythrocytes...
MAMA decoction (MD), a preparation from the leaves of Mangifera indica, Alstonia boonei, Morinda lucida, and Azadirachta was co-administered with amodiaquine, resulted in synergistic clearance malaria parasites previous report [1]. The pharmacokinetic basis for this observation, significant increases exposure half-life desethylamodiaquine, major metabolite reported mice [2]. Here, further evaluation these previously identified herb-drug interactions carried out healthy human volunteers.