A5028902870- PARP inhibition in cancer therapy
- Biosimilars and Bioanalytical Methods
- Health Systems, Economic Evaluations, Quality of Life
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolic Engineering and Bioproduction
- Sirtuins and Resveratrol in Medicine
- Bioinformatics and Genomic Networks
- Muscle Physiology and Disorders
- Histone Deacetylase Inhibitors Research
- Photosynthetic Processes and Mechanisms
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Bacterial Genetics and Biotechnology
- Cancer Cells and Metastasis
- Genomics and Phylogenetic Studies
- Protein Structure and Dynamics
- Gut microbiota and health
- Conducting polymers and applications
- Adipose Tissue and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- ATP Synthase and ATPases Research
- Fungal and yeast genetics research
- Microbial Community Ecology and Physiology
- Plant nutrient uptake and metabolism
- Gene Regulatory Network Analysis
GlaxoSmithKline (Germany)
2018-2023
ETH Zurich
2014-2022
GlaxoSmithKline (United Kingdom)
2020
Life Science Zurich
2014-2017
University of Stavanger
2009
Many microorganisms live in communities and depend on metabolites secreted by fellow community members for survival. Yet our knowledge of interspecies metabolic dependencies is limited to few with small number exchanged metabolites, even less known about cellular regulation facilitating exchange. Here we show how yeast enables growth lactic acid bacteria through endogenous, multi-component, cross-feeding a readily established community. In nitrogen-rich environments, Saccharomyces cerevisiae...
Abstract Understanding how the homeostasis of cellular size and composition is accomplished by different organisms an outstanding challenge in biology. For exponentially growing Escherichia coli cells, it long known that cells exhibits a strong positive relation with their growth rates nutrient conditions. Here, we characterized cell sizes set orthogonal limitations. We report mass exhibit or negative dependences rate depending on limitation applied. In particular, synthesizing large amounts...
Article18 January 2016Open Access Transparent process Life span extension by targeting a link between metabolism and histone acetylation in Drosophila Shahaf Peleg Department of Physiological Chemistry, Biomedical Center for Integrated Protein Science Munich, Ludwig-Maximilians University, Planegg-Martinsried, Germany Molecular Biology, Search more papers this author Christian Feller Ignasi Forne Analysis Unit, Center, Evelyn Schiller Institute Experimental Genetics, Helmholtz Zentrum German...
Abstract Metabolism is one of the best‐understood cellular processes whose network topology enzymatic reactions determined by an organism's genome. The influence genes on metabolite levels, however, remains largely unknown, particularly for many encoding non‐enzymatic proteins. Serendipitously, genomewide association studies explore relationship between genetic variants and but a comprehensive interaction has remained elusive even simplest single‐celled organisms. Here, we systematically...
Cells constantly adapt to unpredictably changing extracellular solute concentrations. A cornerstone of the cellular osmotic stress response is metabolic supply energy and building blocks mount appropriate defenses. Yet, extent which impinges on network remains largely unknown. Moreover, it mostly unclear which, if any, responses are conserved among diverse organisms or confined particular groups species. Here we investigate global twelve bacteria, two yeasts human cell lines exposed...
Abstract 5-Fluorouracil (5-FU) is a widely used chemotherapeutical that induces acute toxicity in the small and large intestine of patients. Symptoms can be severe lead to interruption cancer treatments. However, there limited understanding molecular mechanisms underlying 5-FU-induced intestinal toxicity. In this study, well-established 3D organoid models human colon (SI) were characterize 5-FU transcriptomic metabolomic responses. Clinically relevant concentrations for vitro testing...
<p>Expression proteomic signature</p>
<p>Photo affinity labelling (PAL)</p>
<p>Steroidomics</p>
<p>Transcriptomic signature</p>
<p>Supplementary Materials and Methods</p>
<p>PARP1 PARP2 engagement (ITDR)</p>
<p>panPARP matrix (affinity enrichment chemoproteomics)</p>
<p>Selectivity profile live cells (2D-TPP)</p>
<p>Niraparib matrix (affinity enrichment chemoproteomics)</p>
<p>Co-inhibition of PARP and cholesterol biosynthesis pathway increase efficiency tumor cell killing</p>
<p>Niraparib specific modulation of cholesterol biosynthesis pathway in cancer cell lines</p>
<p>Selectivity profiling and Niraparib-LSS interaction</p>
<p>Niraparib specific modulation of expression levels proteins involved in cholesterol biosynthesis pathway cancer cell lines (proteomics)</p>
<p>Detection of 24,25-epoxycholesterol</p>