A5086930238- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Pancreatic function and diabetes
- Plant Disease Resistance and Genetics
- Chromosomal and Genetic Variations
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Plant Parasitism and Resistance
- Plant Physiology and Cultivation Studies
- Biofuel production and bioconversion
- Nematode management and characterization studies
- Plant Stress Responses and Tolerance
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Plant Growth Enhancement Techniques
Université de Picardie Jules Verne
2007-2022
Biologie des Plantes et Innovation
2008-2021
Institut Charles Viollette
2020
École Polytechnique
2020
UMR Transfrontalière BioEcoAgro
2020
Institut de Génomique Fonctionnelle
2006
University of Leicester
2002-2003
Czech Agrifood Research Center
1992
John Innes Centre
1988-1991
Norwich University
1990
Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. The accuracy the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical algorithms developed recently help validate reference and most studies gene expression mammals, yeast bacteria now include such validation. Surprisingly, important approach is...
• Here, we focused on the biochemical characterization of Arabidopsis thaliana pectin methylesterase 3 gene (AtPME3; At3g14310) and its role in plant development. A combination biochemical, expression, Fourier transform-infrared (FT-IR) microspectroscopy reverse genetics approaches were used. We showed that AtPME3 is ubiquitously expressed A. thaliana, particularly vascular tissues. In cell wall-enriched fractions, only mature part protein was identified, suggesting it processed before...
Pectin methylesterases (PMEs) catalyze the demethylesterification of homogalacturonan domains pectin in plant cell walls and are regulated by endogenous methylesterase inhibitors (PMEIs). In Arabidopsis dark-grown hypocotyls, one PME (AtPME3) PMEI (AtPMEI7) were identified as potential interacting proteins. Using RT-quantitative PCR analysis gene promoter::GUS fusions, we first showed that AtPME3 AtPMEI7 genes had overlapping patterns expression etiolated hypocotyls. The two proteins...
The fine-tuning of the degree methylesterification cell wall pectin is a key to regulating elongation and ultimately shape plant body. Pectin spatiotemporally controlled by methylesterases (PMEs; 66 members in Arabidopsis [Arabidopsis thaliana]). comparably large number proteinaceous methylesterase inhibitors (PMEIs; 76 Arabidopsis) questions specificity PME-PMEI interaction functional role such abundance. To understand difference, or redundancy, between PMEIs, we used molecular dynamics...
The induction of plant somatic embryogenesis is often a limiting step for multiplication and genetic manipulation in numerous crops. It depends on multiple signaling developmental processes involving phytohormones the specific genes. WUSCHEL gene (WUS) required production embryogenic stem cells. To explore different approach to induce embryogenesis, we have investigated effect heterologous ArabidopsisWUS overexpression under control jasmonate responsive vsp1 promoter morphogenic responses...
Abstract Previous work from our laboratory has shown that human ether à go‐go (hEAG) K + channels are crucial for breast cancer cell proliferation and cycle progression. In this study, we investigated the regulation of hEAG by an insulin‐like growth factor‐1 (IGF‐1), which is known to stimulate proliferation. Acute applications IGF‐1 increased current‐density hyperpolarized MCF‐7 cells. The effects were inhibited inhibitors. Moreover, mRNA expression in a time‐dependent manner parallel with...
We have used a polymerase chain reaction (PCR) procedure to analyse low abundance complementary sense RNAs of Digitaria streak virus (DSV) from infected leaves setigera . This study has confirmed that both spliced and unspliced are synthesised by the same transcription unit. The position intron been proven sequencing cDNAs corresponding RNA. Although majority cDNAS 3′ ends at coordinate 1063, downstream consensus polyadenylation sequence, minor population with heterogeneous also identified....
Summary Few Arabidopsis mutants defective in early male or female germline development have been reported. A novel extinction screen has devised which permits the identification of deficient earliest stages anther development. Using mutagenized plants carrying GUS reporter constructs driven by tapetal‐specific promoters originally derived from Brassica genes, a wide spectrum identified , ranging those archesporial cell differentiation to others expressed later Crosses between these lines and...
Journal Article An expression cassette for targeting foreign proteins into chloroplasts Get access Francois Guerineau, Guerineau John Innes Institute, AFRC Institute of Plant Science ResearchColney Lane, Norwich, NR4 7UH, UK Search other works by this author on: Oxford Academic PubMed Google Scholar Sally Woolston, Woolston Louise Brooks, Brooks Phillp Mullineaux Nucleic Acids Research, Volume 16, Issue 23, 9 December 1988, Page 11380, https://doi.org/10.1093/nar/16.23.11380 Published: 09...
The vsp1 gene of Arabidopsis thaliana encodes a storage protein that accumulates in vegetative organs. Transgenic plants expressing promoter–gus (β‐glucuronidase) fusion were found to contain high GUS activity when challenged with jasmonate, volatile plant hormone. induction vsp1–gus expression by jasmonate could be measured tobacco mesophyll protoplasts, after transient expression. A number deletions operated the promoter order locate its jasmonate‐responsive element. 41 bp sequence taken...
The hoc mutant displays high organogenic competence for in vitro shoot regeneration without addition of exogenous phytohormones. genetic basis this phenotype is investigated here. Using mapping, the HOC locus was identified on chromosome 1. A point mutation found At1g52150 gene, which encodes ATHB15/CORONA/INCURVATA4, a class III HD-ZIP transcription factor. replaced serine with cysteine MEKHLA domain protein. wild-type ATHB15 gene able to complement phenotype. Organogenesis response...
Summary A gene encoding a temperature‐sensitive diphtheria toxin chain (DTA) polypeptide was fused to the Arabidopsis thaliana tapetum‐specific A9 promoter. Expression of chimaeric in transgenic A. lines resulted plants that were male‐sterile, but female‐fertile, when grown at 18 °C, and fully self fertile 26 °C. No pollen grains found on anthers although aggregated inside anthers. Electron microscopy revealed discrete alterations tapetal cells male‐sterile plants. The strength phenotype...