A5020203687- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Machine Learning in Materials Science
- Metabolomics and Mass Spectrometry Studies
- Neuroscience and Neuropharmacology Research
- Cutaneous Melanoma Detection and Management
- Neurobiology and Insect Physiology Research
- Cellular Mechanics and Interactions
- Mass Spectrometry Techniques and Applications
- melanin and skin pigmentation
- Dermatologic Treatments and Research
- Optical Imaging and Spectroscopy Techniques
- Photoreceptor and optogenetics research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photochemistry and Electron Transfer Studies
- Image Processing Techniques and Applications
- Skin and Cellular Biology Research
- Protein Structure and Dynamics
- Cutaneous lymphoproliferative disorders research
- Physiological and biochemical adaptations
- Plant Reproductive Biology
- Ocular Infections and Treatments
- Enzyme Structure and Function
- Advancements in Transdermal Drug Delivery
Hospital Damas
2023
Centre National de la Recherche Scientifique
2013-2020
ESPCI Paris
2020
Université Paris-Sud
2013-2016
Laboratoire Aimé Cotton
2014-2016
École Normale Supérieure Paris-Saclay
2014-2016
Université Paris-Saclay
2016
Institut des Sciences Moléculaires d'Orsay
2013-2014
Line-field confocal optical coherence tomography (LC-OCT) is a new, valid means for rapid and non-invasive in vivo examination of the epidermis upper dermis, allowing digital interpretation measurement high-resolution images on cellular level. Given these properties, it may represent tool monitoring psoriasis during treatment, new method to set precise objective severity disease.
Abstract Epidermal three‐dimensional (3D) topography/quantification has not been completely characterized yet. The recently developed line‐field confocal optical coherence tomography (LC‐OCT) provides real‐time, high‐resolution, in‐vivo 3D imaging of the skin. This pilot study aimed at quantifying epidermal metrics (epidermal thicknesses, dermal‐epidermal junction [DEJ] undulation and keratinocyte number/shape/size) using LC‐OCT. For each participant (8 female, skin‐type‐II, younger/older...
Abstract Background Facial dark spots remain a significant challenge for the cosmetic industry, in terms of providing effective treatment. Using Line‐field Confocal Optical Coherence Tomography (LC‐OCT), we investigated internal structural features photo‐aging spot areas and evaluated efficacy skin‐brightening product. Materials Methods Twenty‐six Asian female volunteers, aged between 29 65 years, applied product on their entire face twice day 2 months. LC‐OCT was used to evaluate...
Abstract Background Line‐field confocal optical coherence tomography (LC‐OCT) is an imaging technique providing “optical biopsies” of the skin in real time and non‐invasively. At a center wavelength 1.3 µm, this innovative technology can be applied to dermo‐cosmetic product development due both high image resolution (~2 µm) sufficient penetration (~0.5 mm). Nevertheless, precise dermal area analyzed with LC‐OCT has never been identified. In study, objective was compare images histological...
Quantitative biomarkers of facial skin ageing were studied from one hundred healthy Caucasian female volunteers, aged 20-70 years, using in vivo 3D Line-field Confocal Optical Coherence Tomography (LC-OCT) imaging coupled with Artificial Intelligence (AI)-based quantification algorithms. Layer metrics, i.e. stratum corneum thickness (SC), viable epidermal and Dermal-Epidermal Junction (DEJ) undulation, as well cellular metrics measured for the temple, cheekbone mandible. For all three...
Recent advancements in high-resolution imaging have significantly improved our understanding of microstructural changes the skin and their relationship to aging process. Line Field Confocal Optical Coherence Tomography (LC-OCT) provides detailed 3D insights into various layers, including papillary dermis its fibrous network. In this study, a deep learning model utilizing ResNet-18 network was trained predict chronological age from LC-OCT images 100 healthy Caucasian female volunteers, aged...
How does the concerted activity of neuronal populations shape behavior? Impediments to address this question are primarily due critical experimental barriers. An integrated perspective on large scale neural information processing requires an in vivo approach that can combine advantages exhaustively observing all neurons dedicated a given type stimulus, and simultaneously achieve resolution is precise enough capture individual neuron activity. Current data from observations either restricted...
Disulfide bonds (S-S) play a central role in stabilizing the native structure of proteins against denaturation. Experimentally, identification these linkages peptide and protein characterization remains challenging. UV photodissociation (UVPD) can be valuable tool identifying disulfide linkages. Here, S-S bond acts as chromophore absorption one photon corresponds to σ-σ* transition. We have investigated dynamics protonated cystine, which is dimer two cysteines linked by bridge, at 263 nm...
Knowing the evolution of skin’s response to mechanical solicitations and understanding its origin is important in medicine, surgery, cosmetics. Studies performed vitro ex vivo show that links exist between topographic skin properties collagen elastin fibers network dermis. But, our knowledge, no study shows this link. In we propose a combination experimental tests demonstrate link dermis vivo. The first method consists analyzing relief images. second uses recently developed imaging technique...
The photofragmentation pathways at 263 nm of several small peptides containing tyrosine as the UV chromophore have been characterized using a multi-coincidence technique. A detailed study fragmentation dynamics protonated Glycine-Tyrosine (GYH+), Tyrosine-Glycine (YGH+), Glycine-Tyrosine-Glycine (GYGH+), Alanine-Tyrosine (AYH+) and Tyrosine-Alanine (YAH+) is presented in this paper. Fragmentations occurring or initiated an excited state are distinguished from those after internal conversion...
Genetic tools and especially genetically encoded fluorescent reporters have given a special place to optical microscopy in drosophila neurobiology research. In order monitor neural networks activity, high speed sensitive techniques, with spatial resolution are required. Structured illumination microscopies wide-field approaches sectioning ability. Despite the large progress made introduction of HiLo principle, they did not meet criteria and/or for brain imaging. We report on new...
We present an implementation of a sensorless adaptive optics loop in widefield fluorescence microscope. This setup is designed to compensate for aberrations induced by the sample on both excitation and emission pathways. It allows fast optical sectioning inside living Drosophila brain. detailed characterization system performances. prove that gain brought realizing structured illumination microscopy with down 50 μm deep
The objective was to compare LC-OCT images with histological sections of the same area, in order validate a new method for vivo and non-invasive quantification superficial dermis thickness.
LC-OCT is a recently developed technology for the diagnosis and monitoring of skin pathologies. 3D images with isotropic cellular resolution allow visualization layers tissue characterization at level. A dermoscopic imaging system has been coupled to LC-OCT, allowing localization coverage lesions, together their margins. Finally, segmentations epidermal keratinocyte nuclei performed using artificial intelligence follow-up treatment pathologies in non-invasive quantitative way. These tools...