A5042239489- Veterinary Oncology Research
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
- Sarcoma Diagnosis and Treatment
- Cancer-related gene regulation
- Infectious Diseases and Mycology
- Advanced Radiotherapy Techniques
- Human-Animal Interaction Studies
- Cancer Research and Treatments
- Radiation Therapy and Dosimetry
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Hedgehog Signaling Pathway Studies
- Veterinary Orthopedics and Neurology
- Rabies epidemiology and control
- Advanced MRI Techniques and Applications
- Venous Thromboembolism Diagnosis and Management
- Veterinary Practice and Education Studies
- Angiogenesis and VEGF in Cancer
- TGF-β signaling in diseases
University of Copenhagen
2018-2025
Uppsala University
2014-2025
Frederiksberg Hospital
2023
Science for Life Laboratory
2013-2023
University of Cambridge
2015-2016
University of Glasgow
2009-2010
Royal College of Physicians and Surgeons of Glasgow
2009
Abstract We present GSD_1.0, a high-quality domestic dog reference genome with chromosome length scaffolds and contiguity increased 55-fold over CanFam3.1. Annotation generated existing long short read RNA-seq, miRNA-seq ATAC-seq, revealed that 32.1% of lifted CanFam3.1 gaps harboured previously hidden functional elements, including promoters, genes miRNAs in GSD_1.0. A catalogue canine “dark” regions was made to facilitate mapping rescue. Alignment these is difficult, but we demonstrate...
Sequencing of candidate genes for obesity in Labrador retriever dogs identified a 14 bp deletion pro-opiomelanocortin (POMC) with an allele frequency 12%. The disrupts the β-MSH and β-endorphin coding sequences is associated body weight (per effect 0.33 SD), adiposity, greater food motivation. Among other dog breeds, was only found closely related flat-coat (FCR), where it similarly mutation significantly more common retrievers selected to become assistance than pets. In conclusion, POMC...
Adaptations allowing dogs to thrive on a diet rich in starch, including significant AMY2B copy number gain, constituted crucial step the evolution of dog from wolf. It is however not clear whether this change was associated with initial domestication, or represents secondary shift related subsequent development agriculture. Previous efforts study process were based geographically limited data sets and low-resolution methods, it therefore known what extent adaptations are universal among...
Abstract Osteosarcoma is a debilitating bone cancer that affects humans, especially children and adolescents. A homologous form of osteosarcoma spontaneously occurs in dogs, its differential incidence observed across breeds allows for the investigation tumor mutations context multiple genetic backgrounds. Using whole-exome sequencing dogs from three susceptible (22 golden retrievers, 21 Rottweilers, 23 greyhounds), we found tumors show high frequency somatic copy-number alterations (SCNA),...
FLASH radiotherapy has emerged as a treatment technique with great potential to increase the differential effect between normal tissue toxicity and tumor response compared conventional radiotherapy. To evaluate feasibility of in relevant clinical setting, we have commenced safety study canine cancer patients spontaneous superficial solid tumors or microscopic residual disease, using electron beam our modified linear accelerator. The setup for was established short applicator nominal...
Dogs, with their breed-determined limited genetic background, are great models of human disease including cancer. Canine B-cell lymphoma and hemangiosarcoma both malignancies the hematologic system that clinically histologically similar to non-Hodgkin angiosarcoma, respectively. Golden retrievers in US show significantly elevated lifetime risk for (6%) (20%). We conducted genome-wide association studies lymphoma, identifying two shared predisposing loci. The associated loci located on...
Extant dog and wolf DNA indicates that domestication was accompanied by the selection of a series duplications on Amy2B gene coding for pancreatic amylase. In this study, we used palaeogenetic approach to investigate timing expansion in ancient populations Western Eastern Europe Southwest Asia. Quantitative polymerase chain reaction estimate copy numbers 13 samples, dated between 15 000 4000 years before present (cal. BP). This evidenced an increase copies dogs from as early 7th millennium...
Background FLASH radiotherapy (RT) is a novel method for delivering ionizing radiation, which has been shown in preclinical studies to have normal tissue sparing effect and maintain anticancer efficacy as compared conventional RT. Treatment of head neck tumors with RT commonly associated severe toxicity, hence the potentially makes it especially advantageous treating oral tumors. In this work, objective was study adverse effects dogs spontaneous treated Methods Privately-owned macroscopic...
Summary High amylase activity in dogs is associated with a drastic increase copy numbers of the gene coding for pancreatic amylase, AMY 2B , that likely allowed to thrive on relatively starch‐rich diet during early dog domestication. Although most thus probably digest starch more efficiently than do wolves, vary widely within population, and it not clear how this variation affects individual ability handle nor health. In humans, salivary 1 correlate both levels enzyme activity, high related...
Abstract Biological features of neoplastic disease affecting mammary gland tissue are shared between canines and humans. Research performed in either species has translational value early phase clinical trials with spontaneous could be informative for human trials. The purpose this study was to investigate the somatic genetic aberrations occurring canine neoplasia by exome capture next generation sequencing. Based on 55 tumor-normal pairs we identified PIK3CA gene as most commonly mutated...
Canine mast cell tumours (CMCT) are one of the most common skin in dogs with a major impact on canine health. Certain breeds have higher risk developing tumours, suggesting that underlying predisposing germ-line genetic factors play role development this disease. The largely unknown, although somatic mutations oncogene C-KIT been detected proportion CMCT, making CMCT comparative model for mastocytosis humans where frequent. We performed genome wide association study golden retrievers from...
The interest in the use of medical cannabis has increased recent years both human and veterinary fields. In Denmark, there are no veterinary-licensed or cannabinoid supplements, it is illegal to prescribe sell cannabinoids intended for treatment patients. This study aimed explore unlicensed Danish dogs, by questioning dog owners about usage, indication use, way purchase, their perceived effect treatment. An anonymous online survey was distributed via social media. total number respondents...
Genome wide association studies (GWAS) have been utilized to identify genetic risk loci associated with both simple and complex inherited disorders. Here, we performed a GWAS in Labrador retrievers hip dysplasia body weight. Hip scores were available for 209 genotyped dogs. We identified significantly locus on chromosome 24, three equally SNPs (p = 4.3 × 10
FLASH radiotherapy (RT) has emerged as a promising modality, demonstrating both normal tissue sparing effect and anticancer efficacy. We have previously reported on the safety efficacy of single fraction RT in treatment oral tumors canine cancer patients, showing tumor response but also risk radiation-induced severe late adverse effects (osteoradionecrosis) for doses ≥35 Gy. Accordingly, objective this study was to investigate if high dose is safe treating non-oral tumors.
Canine mammary tumours (CMT) are the most common neoplasia in unspayed female dogs. CMTs suitable naturally occurring models for human breast cancer and share many characteristics, indicating that genetic causes could also be shared. We have performed a genome-wide association study (GWAS) English Springer Spaniel dogs identified significant locus on chromosome 11 (praw = 5.6x10-7, pperm 0.019). The associated haplotype spans 446 kb region overlapping CDK5RAP2 gene. protein has function cell...
Abstract Breast cancer (BC) is a genetically heterogeneous disease with high prevalence in Northern Europe. However, there has been no detailed investigation into the Scandinavian somatic landscape. Here, homogeneous Swedish cohort, we describe events underlying BC, leveraging targeted next-generation sequencing approach. We designed 20.5 Mb array targeting coding and regulatory regions of genes known role BC ( n = 765). The selected were either from human studies 294) or within canine...
Abstract Pyometra is one of the most common diseases in female dogs, presenting as purulent inflammation and bacterial infection uterus. On average 20% intact dogs are affected before 10 years age, a proportion that varies greatly between breeds (3–66%). The clear breed predisposition suggests genetic risk factors involved disease development. To identify associated with disease, we performed genome-wide association study (GWAS) golden retrievers, increased developing pyometra (risk ratio:...
Mast cell tumours are the most common type of skin cancer in dogs, representing a significant concern canine health. The molecular pathogenesis is largely unknown, but breed-predisposition for mast tumour development suggests involvement inherited genetic risk factors some breeds. In this study, we aimed to identify germline associated with Labrador Retrievers, breed an elevated development. Using methodological approach that combined genome-wide association targeted next generation...
Abstract Background During recent years FLASH radiotherapy (FLASH‐RT) has shown promising results in radiation oncology, with the potential to spare normal tissue while maintaining antitumor effects. The high speed of FLASH‐RT delivery increases need for fast and precise motion monitoring avoid underdosing target. Surface guided (SGRT) uses surface imaging (SI) render a 3D patient. SI provides real‐time large scanning field view, covering off‐isocentric positions. However, so far only been...