A5083448635- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Parvovirus B19 Infection Studies
- Virus-based gene therapy research
- Salmonella and Campylobacter epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Protein Structure and Dynamics
- Viral Infections and Vectors
- Immunotherapy and Immune Responses
- Viral Infections and Immunology Research
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Respiratory viral infections research
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- Cancer-related molecular mechanisms research
- Plant Genetic and Mutation Studies
- RNA regulation and disease
- Polyomavirus and related diseases
- Research in Cotton Cultivation
- Pleural and Pulmonary Diseases
University of Bern
2024
SIB Swiss Institute of Bioinformatics
2016-2019
University of Geneva
2016-2017
Geneva College
2016
University Hospital of Geneva
2016
University of Lisbon
2014
University of Trás-os-Montes and Alto Douro
2011-2013
Association for the Development of Douro Viticulture
2011-2013
Universidad de Los Andes
2013
University of the Andes
2009
Abstract Next-generation sequencing has identified novel astroviruses for which a pathogenic role is not clearly defined. We astrovirus MLB2 infection in an immunocompetent case-patient and immunocompromised patient who experienced diverse clinical manifestations, notably, meningitis disseminated infection. The initial was by next-generation sequencing, revealed RNA cerebrospinal fluid, plasma, urine, anal swab specimens. then used specific real-time reverse transcription PCR to screen 943...
Understanding protein-solute interactions is one of the sizable challenges protein chemistry; therefore, numerous experimental studies have attempted to explain mechanism by which proteins unfold in aqueous urea solutions. On basis kinetic evidence at low concentrations, (1)H NMR spectroscopic analysis, and molecular orbital calculations, we propose a mechanistic model for denaturation RNase A urea. Our results support direct interaction between protonated histidine as initial step...
Dendritic cells (DC) are professional antigen presenting playing a major role in orchestrating adaptative immune responses. To adapt to various challenges, such as different classes of pathogens, specialized subsets DC have evolved across species. date, classified conventional (cDC1, cDC2) and plasmacytoid (pDC), with the more recent addition DC3 transitional (tDC) that were discovered human mouse thanks high-dimensional phenotyping single-cell sequencing technologies. Here, by combining...
Unbiased classification of equine dendritic cells (DC) is necessary to address various research questions such as the role DC subsets in immune-mediated diseases horses. We applied single-cell RNA sequencing (scRNA-seq) on enriched from blood two horses, based expression CD172a, Flt3, CADM1 and CD14. All main were detected key gene expression, including cDC type 1 (cDC1; XCR1), cDC2 (FCER1A, CD1E) plasmacytoid (pDC; TCF4). In addition, we a small cluster CD34-expressing progenitors. Our data...
The aim of this study was to report the antimicrobial resistance, molecular mechanisms associated and detection virulence determinants within faecal Enterococcus spp. Escherichia coli isolates Iberian wolf. Enterococci (n = 227) E. 195) were obtained from samples wolf (Canis lupus signatus). High rates resistance detected for tetracycline erythromycin among enterococci isolates, most resistant harboured tet(M) and/or tet(L) erm(B) genes, respectively. blaTEM, tet(A) tet(B), aadA or strA-strB...
BACKGROUND Although the risk of transmitting infectious agents by blood transfusion is dramatically reduced after donor selection, leukoreduction, and laboratory testing, some could still be present in donor's blood. A description metagenomes products eligible for represents relevant information to evaluate pathogen transmission transfusion. STUDY DESIGN AND METHODS Detection viruses, bacteria, fungi genomes was made high‐throughput sequencing (HTS) 600 manufactured transfusion: 300 red cell...
We aimed to determine the prevalence of extended-spectrum beta-lactamase (ESBL)–producing Escherichia coli in fecal samples healthy pigs, and evaluate their clonality associated resistance. Forty-nine percent pigs sampled (n=35/71) a slaughterhouse Portugal revealed ESBL-producing E. isolates. Most isolates produced CTX-M-1 enzyme (71.4%; n=25/35), followed by CTX-M-9 (11.4%; n=4/35), CTX-M-14 (5.7%; n=2/35), SHV-12 CTX-M-32 n=2/35). Ninety-four presented phenotype multi-resistance. belonged...
Aims. Kawasaki disease is an acute pediatric vasculitis whose etiology remains unknown but epidemiology and clinical presentation suggest a viral etiology. We performed unbiased high-throughput-sequencing on blood of patients with Disease (KD). Materials methods. High-throughput-sequencing was directly plasma children typical KD. Sequences were aligned against database clinically relevant viruses. Results. Four acutely infected in the blood, respectively poliovirus (vaccine strain), measles...
There has been concern about the increase of antimicrobial resistant bacteria and protection animal public health, along with food safety. In present study, we evaluate incidence resistance among 192 strains Escherichia coli isolated from faecal samples healthy food-producing animals at slaughter in Portugal.Ninety-seven % pig isolates, 74% sheep 55% cattle were to one or more agents, resistances ampicillin, streptomycin, tetracycline trimethoprim-sulfamethoxazole most common phenotype...
The intensive use of antibiotics in human and veterinary medicine, associated with mechanisms bacterial genetic transfer, caused a selective pressure that contributed to the dissemination antimicrobial resistance different bacteria groups throughout ecosystems. Iberian wolf, due his predatory wild nature, may serve as an important indicator environmental contamination resistant bacteria. aim this study was characterize diversity extended-spectrum β-lactamase (ESBL)-producing Escherichia coli...
Fever is the leading cause of paediatric outpatient consultations in Sub-Saharan Africa. Although most are suspected to be viral origin, a putative causative pathogen not identified over quarter these febrile episodes. Using de novo assembly sequencing approach, we report detection (15.4%) dicistroviruses (DicV) RNA sera collected from 692 Tanzanian children. In contrast, DicV was only detected 1/77 (1.3%) plasma samples adults, suggesting that children could represent primary susceptible...
1. Bacterial resistance to β-lactam antibiotics has risen dramatically in Escherichia coli from food animals. In a previous study, 29 randomly selected chicken products, collected Portugal, were analysed for the presence of extended-spectrum β-lactamases (ESBLs)-producing E. coli; and during this study genetic characterisation ESBLs genes was investigated. 2. The encoding TEM, OXA, SHV, CTX-M type beta-lactamases studied by PCR followed sequencing. Additionally, other mechanisms...
RNA-sequencing (RNA-seq) is rapidly emerging as the technology of choice for whole-transcriptome studies. However, RNA-seq not a bias free technique. It requires large amounts RNA and library preparation can introduce multiple artifacts, compounded by problems from later stages in process. Nevertheless, increasingly used studies, including characterization tissue-specific transcriptomes invertebrate models human disease. The generation samples this context complex, involving establishment...
Meningitis, encephalitis, and myelitis are various forms of acute central nervous system (CNS) inflammation, which can coexist lead to serious sequelae. Known aetiologies include infections immune-mediated processes. Despite advances in clinical microbiology over the past decades, cause CNS inflammation remains unknown approximately 50% cases. High-throughput sequencing was performed search for viral sequences cerebrospinal fluid (CSF) samples collected from 26 patients considered have...
Background and objectives Platelet concentrates are frequently transfused to patients with reduced immunity. An exhaustive description of their viral content is needed prevent unwanted infection. Material methods To track sequences, a shotgun metagenomics approach was used on bank 300 platelets concentrates. Sequences were analysed through the diagnostics‐oriented pipeline ez VIR . Results We only observed viruses commonly described in healthy individuals. Conclusion Herein reported first...
Abstract Next-generation sequencing has identified novel astroviruses for which a pathogenic role is not clearly defined. We astrovirus MLB2 infection in an immunocompetent case-patient and immunocompromised patient who experienced diverse clinical manifestations, notably, meningitis disseminated infection. The initial was by next-generation sequencing, revealed RNA cerebrospinal fluid, plasma, urine, anal swab specimens. then used specific real-time reverse transcription PCR to screen 943...