A5054714221- Protein Interaction Studies and Fluorescence Analysis
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- DNA and Nucleic Acid Chemistry
- Drug Transport and Resistance Mechanisms
- Molecular Sensors and Ion Detection
- Metal complexes synthesis and properties
- Advanced biosensing and bioanalysis techniques
- Nanoparticles: synthesis and applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Berberine and alkaloids research
- Surfactants and Colloidal Systems
- RNA and protein synthesis mechanisms
- Luminescence and Fluorescent Materials
- Carbon and Quantum Dots Applications
- Nanoparticle-Based Drug Delivery
- Advanced Nanomaterials in Catalysis
- Synthesis and Biological Evaluation
- Crystallography and molecular interactions
- Nanocluster Synthesis and Applications
- Synthesis and biological activity
- Synthesis and Characterization of Heterocyclic Compounds
- Electrochemical Analysis and Applications
- Cancer therapeutics and mechanisms
- Crystal structures of chemical compounds
Vidyasagar University
2016-2025
Midnapore Medical College and Hospital
2014-2019
University of Kalyani
2019
Indian Institute of Chemical Biology
2006-2013
Council of Scientific and Industrial Research
2008-2011
The binding of the known DNA intercalators methylene blue and quinacrine with four sequence specific polynucleotides, viz. poly(dG-dC).poly(dG-dC), poly(dG).poly(dC), poly(dA-dT).poly(dA-dT) poly(dA).poly(dT), have been compared using absorbance, fluorescence, competition dialysis thermal melting thermodynamic aspects interaction studied. In all cases, non-cooperative phenomena obeying neighbor exclusion principle was observed though affinity remarkably higher for nature characterized to be...
Background Interaction of the iminium and alkanolamine forms sanguinarine with bovine serum albumin (BSA) was characterized by spectroscopic calorimetric techniques. Methodology/Principal Findings Formation strong complexes BSA both revealed from fluorescence quenching sanguinarine. Binding parameters calculated Stern-Volmer method that neutral had higher affinity to compared charged form. Specific binding distances 3.37 2.38 nm between Trp 212 (donor) (acceptor), respectively, were obtained...
Berberine, palmatine and coralyne, the isoquinoline alkaloids distributed in many botanical families, are extensively investigated due to their potential therapeutic actions clinical utilities. In this work, binding characteristics hemoglobin (Hb) were studied by UV-vis absorption spectroscopy, fluorescence circular dichroism isothermal calorimetric titration differential scanning techniques. The results indicated that all three caused strong quenching of Hb static mechanism, but with...
Abstract In recent years, green synthesized nanoparticles from plant extract have drawn a great interest due to their prospective nanomedicinal application. This study investigates proficient, safer, and sustainable way for the preparation of AgNPs using medicinal Pongamia pinnata (family: Leguminoseae, species: Pinnata ) seeds without any external reducing stabilizing agent. Both ultraviolet‐visible spectrum at λ max = 439 nm energy dispersive X‐ray spectra proof formation AgNPs. An average...
There is compelling evidence that cellular DNA the target of many anticancer agents. Consequently, elucidation molecular nature governing interaction small molecules to paramount progression rational drug design strategies. In this study, we have compared binding and thermodynamic aspects two known DNA-binding agents, quinacrine (QNA) methylene blue (MB), with calf thymus (CT) DNA. The study revealed noncooperative phenomena for both drugs an affinity one order higher QNA MB as observed from...
The structural effects and thermodynamics of the DNA binding six berberine analogues with alkyl chains varying length a terminal phenyl group at C-13 position were investigated. All bound noncooperatively in contrast to cooperative berberine. affinity was higher effect chain only up (CH(2))(3), after which decreased slightly. Intercalative strong stabilization helix revealed. Binding resulted weakening base stacking moderate conformational changes within B-form. entropy driven each case,...
Background Binding of two 9-O-(ω-amino) alkyl ether berberine analogs BC1 and BC2 to the RNA triplex poly(U)•poly(A)*poly(U) was studied by various biophysical techniques. Methodology/Principal Findings Berberine bind non-cooperatively. The affinity binding remarkably high about 5 15 times, respectively, for compared berberine. site size around 4.3 all. Based on ferrocyanide quenching, fluorescence polarization, quantum yield values viscosity results a strong intercalative has been...
The health benefits stemming from green tea are well known, but the exact mechanism of its biological activity is not elucidated. Epicatechin (EC) and epicatechin gallate (ECG) two dietary catechins ubiquitously present in tea. Serum albumins functionally carry these through circulatory system eliminate reactive oxygen species (ROS) induced injury. In study ECG observed to have higher antioxidant activity; which attributed presence galloyl moiety. binding affinity bovine serum albumin (BSA)...
The sequence selectivity of the DNA binding phenazinium dyes phenosafranin and safranin O have been investigated with four sequence-specific deoxyribopolynucleotides from spectroscopic calorimetric studies. alternating guanine−cytosine has revealed affinity values, circular dichroism, thermal melting, competition dialysis, results. affinities both to polynucleotides were order 105 M−1, but values higher for over adenine−thymine ones. Phenosafranin had a compared O. Isothermal titration...
To understand the structure–activity relationship of isoquinoline alkaloids, absorption, fluorescence, circular dichroism, and thermodynamics were employed to study interaction five C-9-ω-amino alkyl ether analogs from plant alkaloid berberine with double-stranded calf thymus DNA. The C-9 derivatization resulted in dramatic enhancements fluorescence emission these compounds. most remarkable changes spectral binding properties BC4 BC5 derivatives. Interactions analogs, which have an...