A5043985840- Ocular Diseases and Behçet’s Syndrome
- Herpesvirus Infections and Treatments
- Retinal Diseases and Treatments
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Retinal Development and Disorders
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Corneal surgery and disorders
- Complement system in diseases
- Glaucoma and retinal disorders
- Ocular Infections and Treatments
- melanin and skin pigmentation
- Retinal Imaging and Analysis
- Autoimmune and Inflammatory Disorders Research
- Systemic Lupus Erythematosus Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal and Optic Conditions
- Immune cells in cancer
- Immunodeficiency and Autoimmune Disorders
- interferon and immune responses
- Protease and Inhibitor Mechanisms
- Advanced Glycation End Products research
- NF-κB Signaling Pathways
- Connexins and lens biology
University Hospital Münster
2023
St. Franziskus Hospital
2011-2020
University of Duisburg-Essen
2005-2011
Augenstern
2004-2005
Essen University Hospital
2003-2005
Microglia represent the primary resident immune cells of central nervous system (CNS) and modulate local responses. Depending on their physiological functions, microglia can be classified into pro- (M1) anti-inflammatory (M2) phenotype. Interleukin (IL)-10 is an important modulator neuronal homeostasis, with neuroprotective released by microglia. Here, we investigated how IL-10 deficiency affected M1/2 polarization upon lipopolysaccharide (LPS) stimulation in vitro. phenotypes were analyzed...
To investigate the effect of human amniotic membrane transplantation (AMT) on T-cell immune response in murine corneas with herpetic stromal keratitis (HSK).Herpes simplex virus (HSV)-1-infected BALB/c mice necrotizing HSK were treated AMT. CD3(+) cell apoptosis was determined and vitro by flow cytometric analysis using annexin V/7-AAD system. The interleukin (IL)-2, cyclosporine, rapamycin, or Fas survival measured. Activation phenotype measured (3)H-thymidine uptake cytometry (CD25, CD69,...
Purpose.: The pathogenesis of juvenile idiopathic arthritis-associated uveitis (JIAU) is undefined. This study intended to analyze the presence antiocular autoantibodies in serum and their correlation with disease course. Methods.: Serum samples from children JIAU (n = 47); JIA without 67); anterior (IAU; n 12); healthy controls 52) were collected. binding patterns antibodies ocular cryosections swine eyes analyzed by indirect immunohistochemistry, correlated epidemiological, clinical,...
To study matrix metalloproteinases (MMP) and tissue inhibitors of (TIMP) in the corneas from mice with ulcerative herpes stromal keratitis (HSK) treated amniotic membrane transplantation (AMT).The BALB/c were infected HSV-1. Mice HSK on postinfection (PI) day 14 used for experiments. In one group mice, (AMT) that was secured a tarsorrhaphy, control underwent tarsorrhaphy alone. After 2 days, appearance corneal ulcers inflammation judged clinically. Corneal sections studied by...
Purpose.: Amniotic membrane transplantation (AMT) reportedly improves herpetic stromal keratitis (HSK). Here we studied the role of amniotic (AM) on macrophages. Methods.: BALB/c mice with necrotizing HSK received an AMT or tarsorrhaphy (TAR) as control. Apoptosis F4/80+ cells was determined using annexinV/7-AAD system. Macrophage invasion a cornea assay. Cytokine secretion quantified by ELISA. Arginase activity measured bioassay. Expression nuclear factor (NF)-κB peroxisome...
A variation in the complement factor H gene was associated with an enhanced risk to develop especially early age-related macular degeneration. Drusen and basal laminar deposits are hallmarks of this AMD manifestation that contain vitronectin as a major component. In study, correlation between stimulation production retinal pigment epithelial (RPE) cells investigated.ARPE-19 cells, permanent cell line human RPE were supplemented without competent serum medium heat inactivated fetal calf...
<b><i>Background/Aims:</i></b> We examined the effect of human complement sera (HCS) on retinal pigment epithelial (RPE) cells with respect to pro-inflammatory mediators relevant in early age-related macular degeneration (AMD). <b><i>Methods:</i></b> RPE were treated complement-containing HCS or heat-inactivated (HI) C7-deficient as controls. Cells analysed for C5b-9 using immunocytochemistry and flow cytometry. Interleukin (IL)-6, IL-8,...
Purpose.: To investigate the effect of systemic or local TNF-α inhibition with etanercept on experimental autoimmune uveoretinitis (EAU). Methods.: EAU was induced by immunizing B10.RIII mice IRBPp161-180 adoptively transferring uveitogenic splenocytes. Mice received treatment in afferent efferent phase. For treatment, were injected intraperitoneally. intravitreally subconjunctivally. Control PBS. scores determined histologically. Splenic cells assessed for [3H]thymidine incorporation. ELISA...
Tumor necrosis factor (TNF)-alpha is a pleiotropic that critical for the development of inflammation. The authors investigated whether topical application TNF-alpha-antagonizing molecules, antisense oligonucleotides (ASON), might be an effective way modifying course immune-mediated herpetic stromal keratitis (HSK).ASON targeting TNF-alpha mRNA were examined their efficiency in interfering with production this cytokine vitro. In vivo uptake was determined by FITC-labeled ASON. HSV-1 corneally...
Age-related macular degeneration (AMD) is accompanied by increased complement activation, and lipofuscin accumulation in retinal pigment epithelial (RPE) cells due to incomplete degradation of photoreceptor outer segments (POS). The influence POS, ultraviolet (UV)-irradiated POS human sera (HCS) on cytokine secretion from RPE was therefore examined.RPE were incubated with or UV-POS every other day for 1 week. autofluorescence (AF) measured photometrically flow cytometry....
Fumaric acid derivates have been shown to stimulate T helper-2-cytokines (interleukin (IL)-4, -5) without affecting the T-helper-1-cytokine (IL-2, interferon (IFN)-gamma)-response. Herein, influence of systemic treatment with fumaric derivate dimethylfumarate (DMF) on secretion helper-cytokines and development HSV-1 stromal keratitis (HSK) was studied in mice. The corneas from BALB/c mice were infected 10(5) PFU (KOS strain). While one group treated intraperitoneally PBS, another received...
Retinal pigment epithelial (RPE) cells undergo functional changes upon complement stimulation, which play a role in the pathogenesis of age-related macular degeneration (AMD). These effects are part enhanced by pretreating ARPE-19 with UV-irradiated photoreceptor outer segments (UV-POS) vitro. The aim this study was to investigate human serum (HCS) treatment on p44/42 mitogen-activated protein kinase (extracellular signal-regulated 1/2 [ERK1/2]) activation pretreated UV-POS.UV-POS-pretreated...
Patients with chronic anterior uveitis are at particularly high risk of developing secondary glaucoma when corticosteroids [e.g., dexamethasone (Dex)] used or inflammatory activity has regressed. Macrophage migration into the eye increases develops and may play an important role in development glaucoma. Our aim was to evaluate vitro if increased hydrostatic pressure could induce changes macrophages phenotype. By using a chamber cell culture system, we assessed effect (HP), inflammation,...