A5052327419- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- IL-33, ST2, and ILC Pathways
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Dermatology and Skin Diseases
- Trypanosoma species research and implications
- Psoriasis: Treatment and Pathogenesis
- Nicotinic Acetylcholine Receptors Study
- Antimicrobial Peptides and Activities
- Cytomegalovirus and herpesvirus research
- Autoimmune and Inflammatory Disorders Research
- Cytokine Signaling Pathways and Interactions
- Vagus Nerve Stimulation Research
- Research on Leishmaniasis Studies
- MicroRNA in disease regulation
- Fibroblast Growth Factor Research
- Bladder and Urothelial Cancer Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Acute Myeloid Leukemia Research
- Histone Deacetylase Inhibitors Research
University of Chicago
2021-2025
University of Illinois Chicago
2021-2024
Experimental Medicine and Biology Institute
2011-2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2011-2022
University of Buenos Aires
2014
Abstract Despite the classical function of NK cells in elimination tumor and virus-infected cells, evidence for a regulatory role has been emerging different models autoimmunity, transplantation, viral infections. However, this not fully explored context growing tumor. In article, we show that can limit spontaneous cross-priming Ag-specific CD8+ T leading to reduced memory responses. After challenge with MC57 transduced express model Ag SIY (MC57.SIY), cell–depleted mice exhibited...
The cellular source of positive signals that reinvigorate T cells within the tumor microenvironment (TME) for therapeutic efficacy programmed death-1 (PD-1)/programmed death-ligand 1 (PD-L1) blockade has not been clearly defined. We now show Batf3-lineage dendritic (DCs) are essential in this process. Flow cytometric analysis, gene-targeted mice, and blocking antibody studies revealed 4-1BBL is a major co-stimulatory signal provided by these DCs TME translates to CD8
IL-27, a member of the IL-12 family cytokines, is produced by APCs, and displays pro- anti-inflammatory effects. How IL-27 affects human NK cells still remains unknown. In this study, we observed that mature DCs secreted blockade IL-27R (CD130) reduced amount IFN-γ during their coculture, showing importance DC-NK-cell crosstalk. Accordingly, rIL-27 stimulated secretion in STAT1-dependent manner, induced upregulation CD25 CD69 on cells, displayed synergistic effect with IL-18. Preincubation...
Abstract NK cells play important roles during immunosurveillance against tumors and viruses as they trigger cytotoxicity susceptible secrete proinflammatory cytokines such IFN-γ. In addition, upon activation, macrophages can become (M1) or anti-inflammatory (M2) cells. Although the consequences of cross-talk between M1 are known, outcome M2 remains ill-defined. Therefore, in current work, we investigated underlying mechanisms interaction resting stimulated human with M2. We observed a lower...
ABSTRACT HDACi are being used as a novel, therapeutic approach for leukemias and other hematological malignancies. However, their effect on immune cells remains ill-defined, may impair surveillance. In this work, we demonstrate that TSA, VPA, NaB inhibited IFN-γ production by CD56dim CD56bright NK cell-mediated cytotoxicity against K562 target cells. promoted minor cell apoptosis but nuclear mobilization of NF-κB p50, which was accompanied robust down-regulation NKG2D NKp46 resting NKG2D,...
Natural Killer (NK) cells play a key role in cancer immunosurveillance. However, NK from patients display an altered phenotype and impaired effector functions. In addition, evidence of regulatory for is emerging diverse models viral infection, transplantation, autoimmunity. Here, we analyzed clear cell renal carcinoma (ccRCC) datasets The Cancer Genome Atlas (TCGA) observed that higher expression signature genes associated with reduced survival. Analysis fresh tumor samples ccRCC unraveled...
Based on the notion that hypomorphic germline genetic variants are linked to autoimmune diseases, we reasoned novel targets for cancer immunotherapy might be identified through associated with greater T-cell infiltration into tumors. Here, report while investigating polymorphisms a tumor immune gene signature, PKCδ as candidate. Genetic deletion of in mice resulted improved endogenous antitumor immunity and increased efficacy anti-PD-L1. Single-cell RNA sequencing revealed myeloid cell...
<div>Abstract<p>Based on the notion that hypomorphic germline genetic variants are linked to autoimmune diseases, we reasoned novel targets for cancer immunotherapy might be identified through associated with greater T-cell infiltration into tumors. Here, report while investigating polymorphisms a tumor immune gene signature, protein kinase C delta (PKCδ) as candidate. Genetic deletion of <i>Prkcd</i> in mice resulted improved endogenous antitumor immunity and...
<p>SNP alleles correlate with an M1/M2 gene signature score</p>
<p>SNP alleles correlate with <i>PRKCD</i> expression and the 160 gene signature score</p>
<p>Hematopoietic loss of PKCδ leads to an altered immune response</p>
<p>Supplemental Table 4</p>
<p>Supplemental Table 6</p>
<p>Supplemental Table 2</p>
<p>Supplemental Table 3</p>
<p>Supplemental Table 5</p>
<p>Hematopoietic loss of PKCδ via Vav1-iCre delays tumor growth and alters inflammatory gene expression in alternative “2C1”Prkcdfl/fl founder.</p>
<p>Overview of the targeting strategy employed for creation <i>Prkcd</i> conditional knockout mice</p>
<p>Supplemental Table 7</p>
<p>T cell priming is unaltered with hematopoietic loss of PKCδ</p>
<p>Hematopoietic loss of PKCδ via Vav1-iCre delays tumor growth and alters inflammatory gene expression in “2B4”Prkcdfl/fl founder.</p>
<p>Supplemental Table 1</p>
<p>Loss of PKCδ in myeloid cells delays tumor growth and is further delayed with anti-PD-L1 therapy multiple Prkcdfl/fl founders.</p>
<p>GSEA reveals many macrophage-related gene sets that are enriched in the C1qa DEG list.</p>