A5044649641- Cancer Research and Treatments
- Metal-Catalyzed Oxygenation Mechanisms
- Amino Acid Enzymes and Metabolism
- Telomeres, Telomerase, and Senescence
- Phytochemical Studies and Bioactivities
- Natural product bioactivities and synthesis
- Enzyme Structure and Function
- Ginseng Biological Effects and Applications
- Genomics and Rare Diseases
- Mesenchymal stem cell research
- Digestive system and related health
- RNA regulation and disease
- Kidney Stones and Urolithiasis Treatments
- Genomics and Chromatin Dynamics
- Trace Elements in Health
- Machine Learning in Bioinformatics
- RNA and protein synthesis mechanisms
- Inflammatory mediators and NSAID effects
- Epigenetics and DNA Methylation
- Bioinformatics and Genomic Networks
- Cancer, Lipids, and Metabolism
- Genetics, Aging, and Longevity in Model Organisms
- Traditional Chinese Medicine Analysis
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
Institute of Molecular and Cell Biology
2017-2024
Ege University
2017-2024
Izmir Institute of Technology
2024
Pharmaceutical Biotechnology (Czechia)
2024
Agency for Science, Technology and Research
2017-2024
Experimental Drug Development Centre
2023
National University of Singapore
2017-2018
Proteins differentially interact with each other across cellular states and conditions, but an efficient proteome-wide strategy to monitor them is lacking. We report the application of thermal proximity coaggregation (TPCA) for high-throughput intracellular monitoring protein complex dynamics. Significant TPCA signatures observed among well-validated protein-protein interactions correlate positively interaction stoichiometry are statistically observable in more than 350 annotated human...
Abstract Transcriptional factors ETS1/2 and p52 synergize downstream of non-canonical NF-κB signaling to drive reactivation the −146C>T mutant TERT promoter in multiple cancer types, but mechanism underlying this cooperativity remains unknown. Here we report crystal structure a ternary p52/ETS1/−146C>T complex. While needs associate with consensus κB sites on DNA function during signaling, show that can activate without binding DNA. Instead, interacts ETS1 form heterotetramer,...
Over-consumption of iron-rich red meat and hereditary or genetic iron overload are associated with increased risk colorectal carcinogenesis, yet the mechanistic basis how metal-mediated signaling leads to oncogenesis remains enigmatic. Using fresh cancer (CRC) samples we identify Pirin, an sensor, that overcomes a rate-limiting step in oncogenesis, by re-activating dormant human-reverse-transcriptase (hTERT) subunit telomerase holoenzyme iron-(Fe3+)-dependent-manner thereby drives CRCs....
Significance Mutations in hTERT promoter are seen over 19% of human cancers, irrespective the cancer type. Understanding molecular players that regulate Mut- promoters may help design effective targeting strategies to inhibit telomerase reactivation specifically cells. Our work uses genome-wide functional screens identify 30 specific regulators promoters. These candidates identified from screening serve as an excellent resource understand how is reactivated and targets for making inhibitors...
<div>Abstract<p>Over-consumption of iron-rich red meat and hereditary or genetic iron overload are associated with an increased risk colorectal carcinogenesis, yet the mechanistic basis how metal-mediated signaling leads to oncogenesis remains enigmatic. Using fresh cancer samples we identify Pirin, sensor, that overcomes a rate-limiting step in oncogenesis, by reactivating dormant human telomerase reverse transcriptase (hTERT) subunit holoenzyme...
<title>Abstract</title> <bold>Purpose</bold> Prostatic inflammation is closely linked to prostate cancer (PCa) and plays a pivotal role in tumor development progression via altering wide range of cellular mechanisms, including proliferation, metastasis, angiogenesis. Since the infiltration immune system cells, such as macrophages, leads higher expression inflammatory mediators microenvironment, use anti-inflammatory drugs could provide valid contribution preventing treating cancer. In our...
<p>ITDRF-CETSA method identifying SP2509’s target.</p>
<p>SP2509 competes with Iron-(Fe<sup>3+</sup>) bound to Pirin</p>
<p>Role of Iron-(Fe<sup>3+</sup>) and Pirin in CRC</p>
<p>Effect of LSD1 on hTERT expression and telomerase activity.</p>
<p>Role of Iron-(Fe<sup>3+</sup>) and Pirin in CRC</p>
<p>Regulation of Sp1 and FBXW7 levels by Pirin SP2509</p>
<p>Iron-(Fe3+) bound to Pirin positively regulates hTERT</p>
<p>Validation of reporter cell lines for small molecule screens.</p>
<p>Iron-(Fe<sup>3+</sup>) regulates hTERT via Pirin-mediated control of FBXW7, the Sp1 targeting E3 ligase</p>
<p>SP2509 mediated regulation of TERT through Pirin</p>
<p>SP2509 mediated inhibition of CRCs occurs due to loss iron-(Fe<sup>3+</sup>) Pirin activity</p>
<p>SP2509’s inhibitory action is specific to CRC specifically CMS3 subtype</p>
<p>SP2509 interaction with Iron-(Fe<sup>3+</sup>)</p>
The cyclin-dependent kinase (CDK) inhibitors purvalanol and roscovitine are therapeutic agents that control cell proliferation through regulating cell-cycle machinery. They also affect polyamine (PA) metabolism, which is activated in malignant tissues. Therefore, PA catabolism became a remarkable target cancer therapies. Induction of the catabolic enzyme spermidine/spermine N1-acetyltransferase (SSAT) under transcription factors such as NF?B PPARγ. purpose this study was to investigate...
Inhibition of inflammation-induced carcinogenesis is an efficient therapeutic strategy for cancer chemoprevention as use anti-inflammatories was reported to decrease the risk. In this study, we aimed investigate inhibition potential semi-synthetic derivations cycloartane-type sapogenol molecules on inflammation-related tumorigenic mechanisms in LNCaP prostate cells. Inflammatory microenvironment stimulated by TNFα/inflammatory conditioned media (CM). WST1/Xcelligence (proliferation),...