Abstract Alzheimer’s disease (AD) is a multifaceted neurodegenerative condition. The pathogenesis of AD highly intricate and the apparent in aged population ~ 50–70 years old. Even after > 100 research, root origin its unclear, complex multifaceted. Herein, we have designed synthesized 9 novel molecules with three different heterocyclic scaffolds namely pyrrolidone-2-one, quinoline & indoline-2-one to imitate explore chemical space around donepezil. were evaluated for their potential...

We investigated the interaction of three flavone compounds, baicalein, chrysin and with calf thymus DNA octamer sequence (CCAATTGG)2. The binding mechanisms compounds both were unveiled using biophysical, thermodynamic molecular modelling techniques. Absorption fluorescence titrations confirm formation complexes along extent interaction. data proposed an intercalation mode binding. Fluorescence displacement assays ethidium bromide Hoechst 33258 supports a partial intercalation. Potassium...

Abstract Changes in mitochondrial dynamics are often associated with dietary patterns, medical treatments, xenobiotics, and diseases. Toxic exposures to hydrogen sulfide (H 2 S) harm mitochondria by inhibiting Complex IV via other mechanisms. However, changes dynamics, including morphology following acute exposure H S, not yet fully understood. This study followed over time after a single LCt 50 dose of S examining electron microscopy thalami images surviving mice. Our findings revealed that...

Stabilizing G-quadruplex structures through small molecule binding is an important area of research in cancer therapy. Cyclic AMP response element-binding protein 1 (CREB1) a transcription factor the CREB family that acts as oncogene. It governs various roles cellular processes, including regulation genes. CREB1 has guanine-rich regions which can form (GQ) structures. Flavones are natural compounds with anticancer properties. We have investigated mode and interaction mechanisms three flavone...

Abstract DNA gyrase brings negative supercoils into and loosens up certain positive that collect during replication transcription is a notable antibacterial target. To fight against the menace of antibiotic-resistant bacterial infections, we have employed various computational tools like high throughput virtual screening (HTVS), standard precision (SP) docking, extra (XP) molecular dynamics (MD) simulation studies to identify some potential inhibitors. A focused library 5968 anti-bacterial...

We report the measurement of optical transport parameters pathologically characterized malignant tissues, normal and different types benign tumors human breast in visible wavelength region. A spatially resolved steady-state diffuse fluorescence reflectance technique was used to estimate values for reduced-scattering coefficient (μs′) absorption (μa) tissues at three wavelengths (530, 550, 590 nm). Different could be well differentiated from one another, also distinguished by their measured...

Mitoxantrone is a promising antitumor drug having considerably reduced cardiotoxicity as compared to anthracyclines. Its binding deoxyhexanucleotides sequence d-(ATCGAT)2 has been studied by proton and phosphorous-31 nuclear magnetic resonance spectroscopy. The stoichiometry reveals that 1:1 2:1 mitoxantrone-d(ATCGAT)2 complexes are formed in solution. Significant upfield shifts 6H/7H, 2H/3H, 11NH, 12NH protons (∼.5 ppm) of mitoxantrone T6NH imino (∼.3 observed. phosphorous resonances do not...

Maya S Nair* & Aishwarya Shukla Department of Biotechnology, Indian Institute Technology Roorkee, India

Development and molecular characterization of polymeric micro-nanofibrous scaffold of a defined 3-D niche for in vitro chemosensitivity analysis against acute myeloid leukemia cells Maya S Nair,1 Ullas Mony,1 Deepthy Menon,1 Manzoor Koyakutty,1 Neeraj Sidharthan,2 Keechilat Pavithran,2 Shantikumar V Nair,1 Krishnakumar N Menon11Amrita Centre Nanosciences Molecular Medicine, 2Department Oncology, Amrita Institute Medical Sciences Research Centre,...

Human papillomavirus 33, a high-risk HPV strain, is mainly responsible for infection and cervical cancer in Asian countries. The E2 protein of 33 DNA-binding that plays crucial role viral replication transcription. We have cloned, overexpressed, purified the DNA binding domain protein. Size exclusion chromatography results suggested exists homodimeric state native form. Circular dichroism data showed has higher content β-sheet. melting temperature obtained from differential scanning...

Fluorescence intensity fluctuations in the visible wavelength regime normal, benign, and cancerous human breast tissue samples are studied through wavelet transform. The analyses have been carried out unpolarized, parallel perpendicularly polarized channels, for optimal characterization. It has observed that fluorescence data, particularly perpendicular components, differentiate various types quite well. Wavelet transform, because of its ability multiresolution analysis, provides ideal tool...

Serum Albumins are the most abundant transport protein and they important in delivery of small molecules drugs to different parts body. Their binding with various ligand has an role pharmaco-kinetic behavior these molecules. Plant derived natural molecules; flavonoids, alkaloids, stilbenes coumarins, possess valuable pharmacological therapeutic potentials. interaction serum albumins is area interest for many researchers. This article reviews coumarins human bovine studied using spectroscopic...

Inhibition of acetylcholinesterase (AChE) has been widely explored to develop novel molecules for management Alzheimer's disease. In past research finding reported molecule 3-(4-(4-fluorobenzoyl)piperidin-1-yl)-1-(4-methoxybenzyl)pyrrolidin-2-one displayed a spectrum anti-Alzheimer's properties herein, we report library 18 that were rationally designed and synthesized employing known literature mimic explore the chemical space around lead compound 6e donepezil. All compounds docked in...

Histones are proteins around which DNA is coiled to form chromatin fibres in the nucleus of a biological cell. They undergo modifications post-translation, process produces using as blueprint. These play very important role regulating gene expression by influencing translation process. The knowledge how such affect and need for an accurate pipeline predict values from modification signals undeniable. In this paper, we present first generalized deep learning model classify based on histone...