A5068240507- Cancer Cells and Metastasis
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Pancreatic and Hepatic Oncology Research
- Metastasis and carcinoma case studies
- Advanced Breast Cancer Therapies
- Cancer Genomics and Diagnostics
- Phagocytosis and Immune Regulation
- RNA modifications and cancer
- TGF-β signaling in diseases
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Renal and related cancers
- Growth Hormone and Insulin-like Growth Factors
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genomic variations and chromosomal abnormalities
- Pancreatic function and diabetes
- Genetic factors in colorectal cancer
- Single-cell and spatial transcriptomics
- Cancer Diagnosis and Treatment
- Pharmacological Effects and Toxicity Studies
- Cancer-related gene regulation
- Cytokine Signaling Pathways and Interactions
- Molecular Biology Techniques and Applications
- Histone Deacetylase Inhibitors Research
The University of Texas Southwestern Medical Center
2018-2022
Vince Lombardi Cancer Clinic
2021
The University of Texas MD Anderson Cancer Center
2019-2021
Southwestern Medical Center
2019
McGill University
2007-2019
Jewish General Hospital
2007-2019
The University of Queensland
2015
QIMR Berghofer Medical Research Institute
2013
McGill University Health Centre
2010
Université de Montréal
2010
Pancreatic ductal adenocarcinoma (PDA) is a major cause of cancer-related death with limited therapeutic options available. This highlights the need for improved understanding biology PDA progression, highly complex and dynamic process featuring changes in cancer cells stromal cells. A comprehensive characterization cell heterogeneity during disease progression lacking. In this study, we aimed to profile populations understand their phenotypic progression. To that end, employed single-cell...
Abstract It has become increasingly clear that the cells within tumor microenvironment play a critical role in cancer growth and metastasis. Studies experimental models suggest carcinoma-associated fibroblasts (CAF) differ from normal are capable of promoting progression through variety mechanisms. At present, definitive view is lacking on whether genomic abnormalities present they might underlie observed phenotypic differences. This study reports molecular analysis largest series breast...
Abstract Background & Aims Pancreatic ductal adenocarcinoma (PDA) is a major cause of cancer-related death with limited therapeutic options available. This highlights the need for improved understanding biology PDA progression. The progression highly complex and dynamic process featuring changes in cancer cells stromal cells; however, comprehensive characterization cell heterogeneity during disease lacking. In this study, we aimed to profile populations understand their phenotypic...
Carcinoma-associated fibroblasts (CAFs) can remodel the extracellular matrix to promote cancer cell invasion, but paracrine signaling between CAFs and cells that regulates tumor migration remains be identified. To determine how interaction modulates invasiveness of cells, we developed a 3-dimensional co-culture model composed breast (BC) MDA-MB-231 spheroids embedded in collagen gel with without CAFs. We found crosstalk promotes invasion by stimulating scattering which was dependent on...
Abstract Purpose: The microenvironment of metastatic breast cancer is incompletely characterized, despite prior evidence that it plays a key role in the biology metastasis. A major component tumor stroma carcinoma-associated fibroblast (CAF), which has been shown to communicate with other stromal and cells create protumorigenic milieu. Our study was designed characterize human CAFs from different sites. Experimental Design: We collected eight fibroblasts (mCAFs) sites compared them primary...
e15739 Background: Pancreatic ductal adenocarcinoma (PDA) is a major cause of cancer-related death with limited therapeutic options available. This highlights the need for improved understanding biology PDA progression. The progression highly complex and dynamic process featuring changes in cancer cells stromal cells; however, comprehensive characterization cell heterogeneity during disease lacking. In this study, we aimed to profile populations understand their phenotypic Methods: We...
Cancer-associated fibroblasts (CAFs) play an important role in breast cancer pathogenesis by paracrine regulation of cell biology. Several vitro and mouse models have characterized the contact cytokine molecules mediating this relationship, although few reports used human CAFs from tumors.Primary CAF cultures were established gene expression profiles analysed order to guide subsequent co-culture models. We a combination colorimetric proliferation assays profiling determine effect on MCF-7...
Recently, we reported that E6/E7 of human papillomavirus (HPV) type 16 cooperates with the ErbB‐2 receptor to induce cellular transformation normal oral epithelial (NOE) and mouse embryonic fibroblast (NEF) cells. Furthermore, demonstrated cyclin D1 is essential for this induced by E6/E7/ErbB‐2 cooperation using antisense knockout (D1 −/– ) To determine role all D‐type cyclins (D1, D2 D3) in cooperation, examined effects E6/E7, alone together NEF, NEF‐D1 , NEF‐D2 NEF‐D3 We confirm NEF‐E6/E7...
Intrahepatic cholangiocarcinoma (ICC) is a primary biliary malignancy that harbors dismal prognosis. Oncogenic mutations of KRAS and loss-of-function BRCA1-associated protein 1 (BAP1) have been identified as recurrent somatic alterations in ICC. However, an autochthonous genetically engineered mouse model ICC genocopies the co-occurrence these has never developed. By crossing Albumin-Cre mice bearing conditional alleles mutant Kras and/or floxed Bap1, Cre-mediated recombination within liver...
733 Background: The genetic heterogeneity of pancreatic ductal adenocarcinoma (PDAC) demands a personalized molecular-targeted treatment approach. While activating KRAS mutations are near ubiquitous event in PDAC pathogenesis, 5-10% cases display deleterious driver the Wnt-signaling negative regulator, ring finger 43 (RNF43). Despite this characterization there no options for subset patients. Methods: We have developed genetically engineered mouse model (GEMM) PDAC, driven by an mutation...
Pancreatic ductal adenocarcinoma (PDAC), a leading cause of cancer-related death in the US, has high metastatic rate that is associated with persistent immune suppression. Axl, member TAM (Tyro3, MerTK) receptor tyrosine kinase family, been identified as critical factor drives metastasis and suppression many cancer types. We have shown pharmacological inhibition Axl significantly reduces tumor progression improves response to chemotherapy multiple preclinical PDAC models. Here we demonstrate...
Abstract RNF43 is an E3 ubiquitin ligase that recurrently mutated in pancreatic ductal adenocarcinoma (PDAC) and precursor cystic neoplasms of the pancreas. The impact mutations on PDAC poorly understood autochthonous models have not been sufficiently characterized. In this study we describe a genetically engineered mouse model (GEMM) with conditional expression oncogenic Kras deletion catalytic domain Rnf43 (KRC) exocrine cells. We demonstrate loss results increased incidence high-grade...
Abstract Intrahepatic cholangiocarcinoma (ICC) is a primary biliary malignancy that harbors dismal prognosis. Oncogenic mutations of KRAS and loss function BRCA1-associated protein 1 ( BAP1 ) have been identified as recurrent somatic alterations in ICC. However, an autochthonous genetically engineered mouse model ICC genocopies the co-occurrence these has never developed. By crossing Albumin -Cre mice bearing conditional alleles mutant Kras and/or floxed Bap1 , Cre-mediated recombination...
<div>Abstract<p>It has become increasingly clear that the cells within tumor microenvironment play a critical role in cancer growth and metastasis. Studies experimental models suggest carcinoma-associated fibroblasts (CAF) differ from normal are capable of promoting progression through variety mechanisms. At present, definitive view is lacking on whether genomic abnormalities present they might underlie observed phenotypic differences. This study reports molecular analysis...
Supplementary Figure 1 from Breast Carcinoma–Associated Fibroblasts Rarely Contain p53 Mutations or Chromosomal Aberrations