A5009596615- Ultrasound and Hyperthermia Applications
- Photoacoustic and Ultrasonic Imaging
- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Ultrasound Imaging and Elastography
- Peptidase Inhibition and Analysis
- Pancreatic and Hepatic Oncology Research
- Nanoplatforms for cancer theranostics
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Biosensors and Analytical Detection
- Cancer Research and Treatments
- Phagocytosis and Immune Regulation
- Neonatal Respiratory Health Research
- Pancreatic function and diabetes
- Erythrocyte Function and Pathophysiology
- Virus-based gene therapy research
- Microfluidic and Capillary Electrophoresis Applications
- Hepatocellular Carcinoma Treatment and Prognosis
- CAR-T cell therapy research
- Advanced MRI Techniques and Applications
- T-cell and B-cell Immunology
- Photodynamic Therapy Research Studies
- Renal and related cancers
- Click Chemistry and Applications
The University of Texas MD Anderson Cancer Center
2020-2021
Stanford Medicine
2018-2020
Stanford University
2015-2020
Palo Alto University
2017-2020
Clark University
2019
University Radiology
2019
RWTH Aachen University
2011-2013
Bayer (Germany)
2013
Federal Office for Radiation Protection
2013
Abstract The combination of target-specific drugs like bevacizumab with chemotherapeutics has improved treatment efficacy in advanced colorectal cancer (CRC). However, the clinical prognosis metastatic CRCs is still poor, and novel are currently assessed respect to their efficacies patients CRCs. In a phase III study, multikinase inhibitor regorafenib (BAY 73-4506) recently been shown prolong survival after standard therapies failed. present activity was investigated comparison angiogenesis...
Advances in precision molecular imaging promise to transform our ability detect, diagnose and treat disease. Here, we describe the engineering validation of a new cystine knot peptide (knottin) that selectively recognizes human integrin αvβ6 with single-digit nanomolar affinity. We solve its 3D structure by NMR x-ray crystallography validate leads 3 different radiolabels pre-clinical models cancer. evaluate lead tracer's safety, biodistribution pharmacokinetics healthy volunteers, show...
Purpose: Breast cancer imaging methods lack diagnostic accuracy, in particular for patients with dense breast tissue, and improved techniques are critically needed.The purpose of this study was to evaluate antibody-indocyanine green (ICG) conjugates, which undergo dynamic absorption spectrum shifts after cellular endocytosis degradation, spectroscopic photoacoustic (sPA) differentiate normal tissue from by B7-H3, a novel associated molecular target.Methods: Quantitative immunohistochemical...
Power Doppler (PD) imaging is a widely used technique for flow detection.Despite the wide use of ultrasound, limitations exist in ability ultrasound to assess slow small-diameter vasculature, such as maternal spiral arteries and fetal villous placenta focal liver lesions.The sensitivity PD small vessel detection limited by low signal produced noise associated with vessels.The sources include electronic noise, stationary or slowly moving tissue clutter, reverberation offaxis scattering from...
Abstract The ability to cryopreserve natural killer (NK) cells has a significant potential in modern cancer immunotherapy. Current cryopreservation protocols cause deterioration NK cell viability and functionality. This work reports the preservation of human cytokine‐activated function following using cocktail biocompatible bioinspired cryoprotectants (i.e., dextran carboxylated ε‐poly‐L‐lysine). Results demonstrate that recovered after rewarming maintain their immediately thawing at...
Molecularly-targeted microbubbles (MBs) are increasingly being recognized as promising contrast agents for oncological molecular imaging with ultrasound.With the detection and validation of new targets, novel binding ligands needed that bind to targets high affinity specificity.In this study we assessed a class potentially clinically translatable MBs using an engineered 10 th type III domain human-fibronectin (MB-FN3VEGFR2) scaffold-ligand image VEGFR2 on neovasculature cancer.The in vitro...
Purpose: Intraoperative near-infrared fluorescence (NIRF) imaging could help stratification for the proper primary treatment patients with pancreatic ductal adenocarcinoma (PDAC), and achieve complete resection, as it allows visualization of cancer in real time. Integrin αvβ6, a target specific PDAC, is present >90% patients, able to differentiate between pancreatitis PDAC. A clinically translatable αvβ6-targeting NIRF agent was developed, based on previously developed cysteine knottin...
Purpose: To engineer a dual human and murine Thy1-binding single-chain-antibody ligand (Thy1-scFv) for contrast microbubble-enhanced ultrasound molecular imaging of pancreatic ductal adenocarcinoma (PDAC).Experimental Design: Thy1-scFv were engineered using yeast-surface-display techniques. Binding to soluble Thy1 Thy1-expressing cells was assessed by flow cytometry. then attached gas-filled microbubbles create MBThy1-scFv binding evaluated under shear stress conditions in flow-chamber...
Abstract Purpose: Human B7-H3 (hB7-H3) is a promising molecular imaging target differentially expressed on the neovasculature of breast cancer and has been validated for preclinical ultrasound (US) with anti–B7-H3-antibody-functionalized microbubbles (MB). However, smaller ligands such as affibodies (ABY) are more suitable design clinical-grade targeted MB. Experimental Design: Binding ABYB7-H3 was confirmed soluble cell-surface by flow cytometry. MB were functionalized or...
Ultrasound molecular imaging (UMI) is enabled by targeted microbubbles (MBs), which are highly reflective ultrasound contrast agents that bind to specific biomarkers. Distinguishing between adherent MBs and background signals can be challenging in vivo. The preferred preclinical technique differential enhancement (DTE), wherein a strong acoustic pulse used destroy verify their locations. However, DTE intrinsically cannot for real-time may cause undesirable bioeffects. In this work, we...
Photoacoustic imaging is a nonionizing biomedical modality with higher resolution and depth than fluorescence imaging, which has greater sensitivity. The combination of the 2 modalities could improve detection cancer. Integrin α<sub>v</sub>β<sub>6</sub> cell surface marker overexpressed in many different cancers. Here, we report development evaluation dye-labeled cystine knot peptide, selectively recognizes integrin high affinity, for photoacoustic imaging. new dual-modality probe may find...
Yeast surface display is a proven tool for the selection and evolution of ligands with novel binding activity. Selections from yeast libraries against transmembrane targets are generally carried out using recombinant soluble extracellular domains. Unfortunately, these molecules may not be good models their true, membrane-bound form variety reasons. Such campaigns often yield that bind target but target-expressing cells or tissues. Advances in cell-based selections aid frequency evolving do...
Using ultrasound to image small vessels in the neonatal brain can be difficult presence of strong clutter from surrounding tissue and with a neonate motion during scan. We propose coherence-based beamforming method, namely short-lag angular coherence (SLAC) that suppresses incoherent noise artifacts Ultrafast data, we demonstrate its applicability improve detection blood flow brain. Instead estimating spatial across receive elements, SLAC utilizes principle acoustic reciprocity estimate...
Ultrasound molecular imaging (USMI) is accomplished by detecting microbubble (MB) contrast agents that have bound to specific biomarkers, and can be used for a variety of applications, such as the early detection cancer. USMI has been widely utilized in preclinical mice; however, humans challenging because low concentration MBs signal degradation caused presence heterogenous soft tissue between transducer lesion. Short-lag spatial coherence (SLSC) beamforming proposed robust technique less...
Objectives Early detection of pancreatic ductal adenocarcinoma (PDAC) represents the most significant step toward treatment this aggressive lethal disease. Previously, we engineered a preclinical Thy1-targeted microbubble (MB Thy1 ) contrast agent that specifically recognizes antigen overexpressed in vasculature murine PDAC tissues by ultrasound (US) imaging. In study, adopted single-chain variable fragment (scFv) site-specific bioconjugation approach to construct clinically translatable MB...
Despite decades of research and clinical trials, metastatic castration-resistant prostate cancer (mCRPC) remains incurable typically fatal. Current treatments may provide modest increases in progression-free survival but can come with significant adverse effects are disaggregated from the diagnostic imaging needed to fully assess spread disease. A theranostic approach, using radiolabeled ligands that target cell surface protein PSMA, simplifies visualization disease treatment process by...
Targeted microbubbles (MBs)enable ultrasound molecular imaging (USMI)by binding to specific biomarkers and producing strong reflections ultrasound. However, current USMI techniques are not easily translatable for clinical use. In particular, preclinical studies often utilize destruction-subtraction imaging, wherein a destructive pulse is used destroy MBs confirm their locations. This approach potentially unsafe, intrinsically real-time. this work, novel neural-network-based beamformer...