A5028646315- Medical Imaging Techniques and Applications
- Radiopharmaceutical Chemistry and Applications
- Cancer, Hypoxia, and Metabolism
- Radiomics and Machine Learning in Medical Imaging
- Nanoplatforms for cancer theranostics
- Peptidase Inhibition and Analysis
- Cancer Research and Treatments
- Pharmacological Receptor Mechanisms and Effects
- Glioma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Receptor Mechanisms and Signaling
- Nanoparticle-Based Drug Delivery
- Advanced MRI Techniques and Applications
- Medical Imaging and Pathology Studies
- Neuropeptides and Animal Physiology
- Cancer, Lipids, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Neuroscience and Neuropharmacology Research
- Prostate Cancer Treatment and Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Cancer Immunotherapy and Biomarkers
- Virus-based gene therapy research
- HER2/EGFR in Cancer Research
- Cerebrovascular and Carotid Artery Diseases
Stanford University
2014-2023
Stanford Medicine
2007-2020
Indiana University School of Medicine
2019
Palo Alto University
2018
Stanford Health Care
2011-2016
National Institute of Mental Health
2004-2014
National Institutes of Health
2004-2014
Hiroshima University
2013
Cardiovascular Institute of the South
2013
Lawrence Berkeley National Laboratory
2011
Glu-NH-CO-NH-Lys-(Ahx)-[<sup>68</sup>Ga(HBED-CC)] (<sup>68</sup>Ga-PSMA-11) is a PET tracer that can detect prostate cancer relapses and metastases by binding to the extracellular domain of PSMA. <sup>68</sup>Ga-labeled DOTA-4-amino-1-carboxymethyl-piperidine-d-Phe-Gln-Trp-Ala-Val-Gly-His-Sta-Leu-NH2 (<sup>68</sup>Ga-RM2) synthetic bombesin receptor antagonist targets gastrin-releasing peptide receptors. We present pilot data on biodistribution these tracers in small cohort patients with...
Purpose To assess the safety, biodistribution, and dosimetric properties of positron emission tomography (PET) radiopharmaceutical agent fluorine 18 (18F) FPPRGD2 (2-fluoropropionyl labeled PEGylated dimeric RGD peptide [PEG3-E{c(RGDyk)}2]), which is based on arginine-glycine–aspartic acid (RGD) sequence targets αvβ3 integrin, in first volunteers imaged with this tracer. Materials Methods The protocol was approved by institutional review board, written informed consent obtained from all...
Drug Design: An (18)F-labeled caspase-3-sensitive nanoaggregation positron emission tomography tracer was prepared and evaluated for imaging the caspase-3 activity in doxorubicin-treated tumor xenografts. Enhanced retention of (18)F apoptotic tumors is achieved through intramolecular macrocyclization situ aggregation upon activation (see picture).
Arterial spin labeling (ASL) MRI is a promising, noninvasive technique to image cerebral blood flow (CBF) but difficult use in cerebrovascular patients with abnormal, long arterial transit times through collateral pathways. To be clinically adopted, ASL must first optimized and validated against reference standard these challenging patient cases.We compared standard-delay (post-label delay=2.025 seconds), multidelay delay=0.7-3.0 long-label long-delay acquisitions delay=4.0 seconds)...
Advances in precision molecular imaging promise to transform our ability detect, diagnose and treat disease. Here, we describe the engineering validation of a new cystine knot peptide (knottin) that selectively recognizes human integrin αvβ6 with single-digit nanomolar affinity. We solve its 3D structure by NMR x-ray crystallography validate leads 3 different radiolabels pre-clinical models cancer. evaluate lead tracer's safety, biodistribution pharmacokinetics healthy volunteers, show...
Abstract The derivation of dopamine neurons is one the best examples clinical potential embryonic stem (ES) cells, but long-term function grafted has not been established. Here, we show that, after transplantation into an animal model, derived from mouse ES cells survived for over 32 weeks, maintained midbrain markers, and had sustained behavioral effects. Microdialysis in animals showed that (DA) release was induced by depolarization pharmacological stimulants. Positron emission tomography...
The cell adhesion molecule integrin αvβ3 plays a key role in tumor angiogenesis and metastasis. A series of 18F-labeled RGD peptides have been developed for PET expression based on primary amine reactive prosthetic groups. In this study, we report the use Cu(I)-catalyzed Huisgen cycloaddition, also known as click reaction, to label with 18F by forming 1,2,3-triazoles. Nucleophilic fluorination toluenesulfonic alkyne provided 18F-alkyne high yield (nondecay-corrected yield: 65.0 ± 1.9%,...
In vivo imaging of α<sub>v</sub>β<sub>3</sub> expression has important diagnostic and therapeutic applications. Multimeric cyclic RGD peptides are capable improving the integrin α<sub>v</sub>β<sub>3</sub>–binding affinity due to polyvalency effect. Here we report an example <sup>18</sup>F-labeled tetrameric peptide for PET in both xenograft spontaneous tumor models. <b>Methods:</b> The E{E[c(RGDyK)]<sub>2</sub>}<sub>2</sub> was derived with amino-3,6,9-trioxaundecanoic acid (mini-PEG; PEG is...
σ-1 receptor (S1R) radioligands have the potential to detect and monitor various neurological diseases. Herein we report synthesis, radiofluorination, evaluation of a new S1R ligand 6-(3-fluoropropyl)-3-(2-(azepan-1-yl)ethyl)benzo[d]thiazol-2(3H)-one ([(18)F]FTC-146, [(18)F]13). [(18)F]13 was synthesized by nucleophilic fluorination, affording product with >99% radiochemical purity (RCP) specific activity (SA) 2.6 ± 1.2 Ci/μmol (n = 13) at end synthesis (EOS). Positron emission tomography...
Abstract Purpose: An early readout of tumor response to therapy through measurement drug or radiation-induced cell death may provide important prognostic indications and improved patient management. It has been shown that the uptake 18F-C-SNAT can be used detect in tumors by positron emission tomography (PET) via a mechanism caspase-3–triggered nanoaggregation. Experimental Design: Here, we compared preclinical utility for detection drug-induced clinically evaluated radiotracers, 18F-FDG,...
Purpose (S)-4-(3-[18F]Fluoropropyl)-L-glutamic acid (18F-FSPG) is a novel radiopharmaceutical for Positron Emission Tomography (PET) imaging. It glutamate analogue that can be used to measure xC- transporter activity. This study was performed assess the feasibility of 18F-FSPG imaging orthotopic brain tumors in small animals and translation this approach human subjects with intracranial malignancies. Experimental Design For animal study, GS9L glioblastoma cells were implanted into brains...
Carboxylic esters were successfully labeled with one of two short-lived positron-emitters, carbon-11 or fluorine-18, within a hydrodynamically-driven micro-reactor. The non-radioactive methyl ester 4a was obtained at room temperature; its yield increased higher substrate concentration and reduced infusion rate. Radioactive 4b from the reaction 1 (10 mM) 2b in 56% decay-corrected radiochemical (RCY) an rate 10 µL min−1, when to RCY 88%. synthesis fluoroethyl 5a 3a required heating...
An efficient method based on a rapid condensation reaction between 2-cyanobenzothiazole (CBT) and cysteine has been developed for (18)F-labeling of N-terminal cysteine-bearing peptides proteins. (18)F-labeled dimeric cRGD ([(18)F]CBTRGD(2)) synthesized with an excellent radiochemical yield (92% radio-HPLC conversion, 80% decay-corrected, isolated yield) purity (>99%) under mild conditions using (18)F-CBT, shown good in vivo tumor targeting efficiency PET imaging. The labeling strategy was...
Cancer cells reprogram their metabolism to meet increased biosynthetic demands, commensurate with elevated rates of replication. Pyruvate kinase M2 (PKM2) catalyzes the final and rate-limiting step in tumor glycolysis, controlling balance between energy production synthesis metabolic precursors. We report here evaluation a positron emission tomography (PET) radiotracer, [(11)C]DASA-23, that provides direct noninvasive measure PKM2 expression preclinical models glioblastoma multiforme (GBM)....
The epidermal growth factor receptor (EGFR) serves as an attractive target for cancer molecular imaging and therapy. Our previous positron emission tomography (PET) studies showed that the EGFR-targeting affibody molecules (64)Cu-DOTA-ZEGFR:1907 (18)F-FBEM-ZEGFR:1907 can discriminate between high low EGFR-expression tumors have potential patient selection EGFR-targeted Compared with (64)Cu, (18)F may improve of is more suitable clinical application, but labeling reaction requires a long...
15 O-H 2 O PET imaging is an accurate method to measure cerebral blood flow (CBF) but it requires arterial input function (AIF). Historically, image-derived AIF estimation suffers from low temporal resolution, spill-in, and spill-over problems. Here, we optimized tracer dose on a time-of-flight PET/MR according the acquisition-specific noise-equivalent count rate curve. An of 850 MBq was determined, which allowed sufficient counts reconstruct short time-frame angiogram (PETA) during phase....
Early detection of tumor response to therapy is crucial the timely identification most efficacious treatments. We recently developed a novel apoptosis imaging tracer, <sup>18</sup>F-C-SNAT (C-SNAT caspase-sensitive nanoaggregation tracer), that undergoes an intramolecular cyclization reaction after cleavage by caspase-3/7, biomarker apoptosis. This caspase-3/7–dependent leads enhanced accumulation and retention <sup>18</sup>F activity in apoptotic tumors. study aimed fully examine vivo...
Abstract Major depressive disorder (MDD) is characterized by the altered integration of reward histories and reduced responding striatum. We have posited that this striatal activation in MDD due to tonically decreased stimulation dopamine synapses which results decremented propagation information along cortico-striatal-pallido-thalamic (CSPT) spiral. In present investigation, we tested predictions formulation conducting concurrent functional magnetic resonance imaging (fMRI) 11 C-raclopride...
The ability to locate nerve injury and ensuing neuroinflammation would have tremendous clinical value for improving both the diagnosis subsequent management of patients suffering from pain, weakness, other neurologic phenomena associated with peripheral injury. Although several non-invasive techniques exist assessing manifestations morphological aspects injury, they often fail provide accurate diagnoses due limited specificity and/or sensitivity. Herein, we describe a new imaging strategy...