The efficiency of RNA interference (RNAi) varies considerably among different insect species, and there is growing evidence to suggest that degradation double-stranded (dsRNA) prior uptake an important factor limits the RNAi in insects. In Locusta migratoria, highly efficient when dsRNA delivered by injection, but not feeding. However, detailed mechanisms causing such differential are still elusive.We identified characterized full-length complementary DNAs (cDNAs) two new nuclease (dsRNase)...

The efficiency of RNA interference (RNAi) varies substantially among different insect species. Rapid degradation double-stranded (dsRNA) by dsRNA-degrading nucleases (dsRNases) has been implicated to cause low RNAi in several In this study, we identified four dsRNase genes (OfdsRNase1, OfdsRNase2, OfdsRNase3 and OfdsRNase4) from the Asian corn borer (Ostrinia furnacalis) transcriptome database. Bioinformatic analyses showed that each deduced protein sequence contained endonuclease NS domains...

During sunflower growth, cold waves often occur and impede plant growth. Therefore, it is crucial to study the underlying mechanism of resistance in sunflowers. In this study, physiological analysis revealed that as stress increased, levels ROS, malondialdehyde, ascorbic acid, dehydroascorbic acid activities antioxidant enzymes increased. Transcriptomics further identified 10,903 DEGs between any two treatments. Clustering demonstrated expression MYB44a, MYB44b, MYB12, bZIP2 bZIP4...

Abstract BACKGROUND Targeting insect‐specific genes through post‐transcriptional gene silencing with RNA interference (RNAi) is a new strategy for insect pest management. However, lepidopterans are recalcitrant to RNAi, which prevents application of novel RNAi technology many notorious pests, including Ostrinia nubilalis (ECB). Strategies enhancing efficiency, large doses double‐stranded (dsRNA), nuclease inhibitors, transfection reagents, and nanoparticles, have proved useful in other...

BACKGROUND The prognosis of idiopathic pulmonary fibrosis (IPF) is the worst among all interstitial lung diseases, and related to disease itself. Comorbidities or complications can worsen IPF. We assessed effect comorbidities on survival IPF patients. A retrospective review patients with was completed. MATERIAL AND METHODS Information demographic features, clinical examination, at baseline were obtained. Then, median, 1-year, 5-year calculated. total 380 admitted Beijing Chao-Yang Hospital...

Variable RNA interference (RNAi) efficiencies limit RNAi-based pest management strategies for many pests. Previous efforts to understand mechanisms contributing low RNAi efficiency indicate that double-stranded (dsRNA) is degraded in the European corn borer (ECB), Ostrinia nubilalis, due nuclease activity. To investigate contribution of dsRNA-degrading endonucleases (dsRNases) and lepidopteran-specific efficiency-related nucleases (REases) dsRNA instability ECB, five complementary DNAs...

Insect chitinases are involved in degradation of chitin from the exoskeleton or peritrophic metrix midgut. In Locusta migratoria, two duplicated Cht5s (LmCht5-1 and LmCht5-2) have been shown to distinct molecular characteristics biological roles. To explore protein properties LmCht5s, we heterologously expressed both enzymes using baculovirus expression system SF9 cells, characterized kinetic carbohydrate-binding purified enzymes. LmCht5-1 LmCht5-2 exhibited similar pH temperature optimums....

Idiopathic pulmonary fibrosis (IPF) is a fatal interstitial lung disease characterized by variable progression. The prealbumin (PA) parameter in routine blood biochemistry examination. We sought to investigate the prognostic value IPF patients.To evaluate prognosis patients with IPF.Blood examination, demographics, function data from consulted Beijing Chao-Yang Hospital and China-Japan Friendship between July 2012 December 2016 were collected. Infection, liver kidney dysfunction...

Glucocorticoid-induced osteoporosis is the most common secondary cause of osteoporosis, which increases risk fracture. Long non-coding RNA telomerase elements (TERC) has been proven to be closely related osteoporosis. However, role TERC in glucocorticoid-induced and its underlying molecular mechanism remains unclear.The vitro model was established after bone marrow mesenchymal stem cells (BMSCs) were exposed dexamethasone (DEX). The cell viability, alkaline phosphatase (ALP) activity...

RNA interference (RNAi) is a sequence-specific gene silencing mechanism that holds great promise for effective management of agricultural pests. Previous studies have shown the efficacy RNAi varies among different insect species, which limits its wide spread application in field crop protection. In this study, we identified and characterized six core pathway genes including OfDicer1, OfDicer2, OfR2D2, OfAgo1, OfAgo2, OfAgo3 from transcriptomic database Asian corn borer (Ostrinia furnacalis)....

Lung adenocarcinoma (LUAD) is the main subtype of non-small cell lung cancer with a poor survival prognosis. In our study, gene expression, DNA methylation, and clinicopathological data primary LUAD were utilized to identify potential prognostic markers for LUAD, which recruited from The Cancer Genome Atlas (TCGA) database. Univariate regression analysis showed that there 21 methylation-associated DEGs related overall (OS), including 9 down- 12 up-regulated genes. genes hypomethylation may...

Intelligent transportation systems (ITSs) present new opportunities for enhanced traffic management by leveraging advanced driving behavior sensors and real-time information exchange via vehicle-based cloud-vehicle communication technologies. Specifically, onboard can effectively detect whether human-driven vehicles are adhering to directives. However, the formulation validation of effective strategies vehicle implementation rely on accurate models reliable model-based testing; in this...

Abstract High specificity for silencing target genes and single‐copy that yield clear phenotypes are two important factors the success of RNA interference (RNAi). The lethal giant larvae ( Lgl ) gene appears to be an ideal RNAi because can effectively suppress its expression results in molting defects mortality Tribolium castaneum . To investigate suitability this other insects, we identified characterized DvLgl from western corn rootworm, Diabrotica virgifera , a species exhibiting high...

Maize (Zea mays L.) kernel width is one of the most important traits that related to yield and appearance. To understand its genetic mechanisms more clearly, a recombinant inbred line (RIL) segregation population consisting 239 RILs was used for quantitative trait locus (QTL) mapping width. We found four QTLs on chromosomes 3 (one), 5 (two), 10 (one). The were close their adjacent markers, with range 0-23.8 cM, explained 6.2-19.7% phenotypic variation. three had positive additive effects,...

Abstract Background The difference in expression of sputum cells types between the two main chronic obstructive pulmonary disease (COPD) is largely unknown. This study aims to investigate and then wants depend on sorting direct treatment COPD. Methods A total 218 patients with stable COPD (FEV1 < 80%) were selected. Stable ( n = 218) classified as eosinophilic (A phenotype) or noneosinophilic (B phenotype); sometimes, subjects separated into cough group (C dyspnea (D phenotype)....

Cigarette smoke can product some different degrees of immediate and long-term hazards on smokers non-smokers.In 2002, a national investigation about smoking showed that the number smokers, tobacco production consumption in China were ranked front world presented an upward trend, meanwhile awareness harm our population was seriously inadequate.This article systematically reviews to improve conscious people resist tobacco, maintain health, be away from related disease. Key words:...