A5005867912- Antimicrobial Resistance in Staphylococcus
- Microbial Metabolism and Applications
- Surgical site infection prevention
- Bacterial Identification and Susceptibility Testing
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Orthopedic Infections and Treatments
- RNA modifications and cancer
- Microbial infections and disease research
- Hepatitis Viruses Studies and Epidemiology
- Otolaryngology and Infectious Diseases
- RNA and protein synthesis mechanisms
- Microscopic Colitis
- Allergic Rhinitis and Sensitization
- Inflammatory Bowel Disease
- Plant Pathogens and Fungal Diseases
- Pediatric Hepatobiliary Diseases and Treatments
- Dermatology and Skin Diseases
- Congenital Anomalies and Fetal Surgery
- Probiotics and Fermented Foods
- Oral microbiology and periodontitis research
- Cancer-related gene regulation
- Fluoride Effects and Removal
Statens Serum Institut
2019-2023
University of Copenhagen
2019
Abstract Methylation of guanosine on position N7 (m7G) internal RNA positions has been found in all domains life and have implicated human disease. Here, we present m7G Mutational Profiling sequencing (m7G-MaP-seq), which allows high throughput detection modifications at nucleotide resolution. In our method, modified are converted to abasic sites by reduction with sodium borohydride, directly recorded as cDNA mutations through reverse transcription sequenced. We detect increased mutation...
Over the last decade, an increasing number of infections with livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398) in persons without contact to livestock has been registered Denmark. These have suggested be result repeated spillover random isolates from into community. However, other studies also found emerging sub-lineages spreading among humans. Based on genome-wide SNPs and association (GWAS), we assessed population structure genomic content...
The skin microbiota of atopic dermatitis (AD) patients is characterized by increased Staphylococcus aureus colonization, which exacerbates disease symptoms and has been linked to reduced bacterial diversity. Skin communities in AD have mostly described at family genus levels, while species-level characterization limited. In this study, we investigated the role bacteria belonging using targeted sequencing tuf gene with genus-specific primers. We compared staphylococcal on lesional...
The aim was to study alterations of bacterial communities in patients undergoing hip or knee arthroplasty assess the impact chlorhexidine gluconate soap decolonisation and systemic antibiotic prophylaxis. A Swedish multicentre, prospective collection samples obtained from elective (n = 83) by swabbing anterior nares, skin sites groin site planned surgery, before after analysed 16S rRNA (V3-V4) gene sequencing a complementary targeted tuf approach comprehensively characterise staphylococcal...
Background/Aim Microbial dysbiosis in inflammatory bowel disease (IBD) is poorly understood. Faecal samples collected for the purposes of microbiota analysis are not yet a part everyday clinical practice. To explore associations between faecal and activity measures adult IBD patients, purpose possibly integrating an existing eHealth application disease-monitoring.Methods We from patients one year while they were home-monitoring activity, using calprotectin (FC) Simple Clinical Colitis...
Abstract Background Although generally known as a human commensal, Staphylococcus epidermidis is also an opportunistic pathogen that can cause nosocomial infections related to foreign body materials and immunocompromized patients. Infections are often caused by multidrug-resistant (MDR) lineages difficult costly treat, have major adverse impact on patients’ quality of life. Heterogeneity common phenomenon in both carriage infection, but present methodology for detection this laborious or...
Dolosigranulum pigrum-a lactic acid bacterium that is increasingly recognized as an important member of the nasal microbiome. Currently, there are limited rapid and low-cost options for confirming D. pigrum isolates detecting in clinical specimens. Here we describe design validation a novel PCR assay targeting both sensitive specific. We designed murJ, single-copy core species gene identified through analysis 21 whole genome sequences. The achieved 100% sensitivity specificity against...
The aim was to study alterations of bacterial communities in patients undergoing hip or knee arthroplasty assess the impact chlorhexidine gluconate soap decolonisation and systemic antibiotic prophylaxis. A Swedish multicentre, prospective collection samples obtained from elective (n=83) by swabbing anterior nares, skin sites groin site planned surgery, before after analysed 16S rRNA (V3-V4) gene sequencing a complementary targeted tuf approach comprehensively characterise staphylococcal...
Abstract Background Although generally known as a human commensal, Staphylococcus epidermidis is also an opportunistic pathogen that can cause nosocomial infections related to foreign body materials and immunicompromized patients. Infections are often caused by multidrug resistant (MDR) lineages difficult costly treat, have major adverse impact on patients’ quality of life. Heterogeneity common phenomenon in both carriage infection, but present methodology for detection this laborious or...