A5051653257- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Contact Dermatitis and Allergies
- Nail Diseases and Treatments
- Gut microbiota and health
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Complementary and Alternative Medicine Studies
- Food Allergy and Anaphylaxis Research
- Bacterial Identification and Susceptibility Testing
- Acne and Rosacea Treatments and Effects
- IL-33, ST2, and ILC Pathways
- Antimicrobial Resistance in Staphylococcus
- Biomedical Ethics and Regulation
- Radiomics and Machine Learning in Medical Imaging
- Stress Responses and Cortisol
- Psoriasis: Treatment and Pathogenesis
- Exercise and Physiological Responses
- Pluripotent Stem Cells Research
- Medical Imaging Techniques and Applications
- Science, Research, and Medicine
- Face Recognition and Perception
- Historical Studies on Reproduction, Gender, Health, and Societal Changes
- Herbal Medicine Research Studies
Bispebjerg Hospital
2013-2023
University of Copenhagen
2013-2023
National University Hospital of Iceland
2009
Atopic dermatitis (AD) is a prevalent disease with significant impact on physical health and quality of life. Staphylococcus aureus has been directly correlated to severity, may also be contributing causal factor in the pathogenesis AD.The primary aim was assess differences S. colonization patients AD without filaggrin gene mutations. The secondary severity relation colonization. Exploratory analyses were performed investigate genetic lineages (FLG) mutations severity.Adult (n = 101)...
Abstract Background Atopic dermatitis ( AD ) affects quality of life (QoL) negatively in patients and their families. We examined the relationship between disease severity QoL with . Methods Consecutive, newly referred outpatients , 4 years age or older, were assessed from January 2012 onwards by means dermatology index DLQI range 0–30), Scoring SCORAD score (range 0–103), filaggrin gene FLG mutation status paraclinical tests related to allergy. Results A total 250 a mean 26.0 identified...
Tape-stripping is a minimally invasive approach for skin sampling that captures the cutaneous immune/barrier abnormalities in atopic dermatitis (AD). However, tape-strips have not been used to evaluate molecular changes with therapeutic targeting. In this study, we sought characterize proteomic signature of from AD patients, before and after dupilumab therapy. Twenty-six patients were treated every-other-week 300mg 16 weeks. Tape-strips lesional non-lesional collected treatment, analyzed...
Questionnaires are widely used in epidemiological studies to measure eczema symptom prevalence, but there concerns regarding their accuracy if as a diagnostic tool.To compare the performance of validated questionnaire and standardized skin examination protocol employed second phase International Study Asthma Allergies Childhood (ISAAC).A total 30,358 schoolchildren aged 8-12 years from 18 countries were examined for flexural eczema. Parents also completed an questionnaire. We compared...
Abstract The aim of this study was to investigate the amount protein in stratum corneum atopic dermatitis (AD) patients and healthy controls, using tape stripping technique. Furthermore, compare two different methods for assessment. Tape performed AD controls collect samples subsequently analysed with methods: Squame Scan, which gives an estimate total (soluble insoluble) Micro BCA determination kit measures soluble protein. Significant differences cumulative content between lesional,...
The skin microbiota of atopic dermatitis (AD) patients is characterized by increased Staphylococcus aureus colonization, which exacerbates disease symptoms and has been linked to reduced bacterial diversity. Skin communities in AD have mostly described at family genus levels, while species-level characterization limited. In this study, we investigated the role bacteria belonging using targeted sequencing tuf gene with genus-specific primers. We compared staphylococcal on lesional...
To evaluate the performance of a cefoxitin 30 microg disc on Iso-Sensitest agar, using semi-confluent inoculum and overnight incubation at 35-36 degrees C, for detection methicillin-resistant Staphylococcus aureus (MRSA).A total 457 S. aureus, including 190 MRSA several defined PFGE types number low-level resistant isolates, were tested with C. This method was compared standard SRGA (Swedish Reference Group Antibiotics) (oxacillin 1 agar supplemented 5% defibrinated horse blood, confluent...
Skin infections related to disrupted antimicrobial defence are a common problem in atopic dermatitis (AD). Altered levels of peptides, including human β-defensin (hBD)-2, have been reported AD skin, and link impaired barrier function has suggested.To study hBD-2 relation skin patients with controls, disease severity.Twenty-five 11 controls were enrolled. peptide concentration was determined stratum corneum samples collected by minimally invasive tape-stripping method. Disease severity...
Abstract The tape stripping technique is increasingly used in research regarding skin barrier function. However, number of strips varies between studies, and literature considering advancement into stratum corneum/epidermis relation to scarce. aim this pilot study was assess the through epidermis using healthy volunteers. A total ten volunteers were included. From all 0, 5, 15 35 consecutive (D-squame) taken from four adjacent areas on middle volar forearm, followed by Reflectance Confocal...
Investigation of changes in the skin microbiome following treatment atopic dermatitis (AD) with dupilumab may provide valuable insights into as a therapeutic target. The aim this study is to assess AD (n = 27). E-swabs were collected from nose, lesional, and nonlesional before after 16 weeks therapy, was analyzed by 16S rRNA tuf gene sequencing. Data for 17 patients milder disease receiving non-targeted therapies are also presented. results show that both groups experienced clinical...
Decreased levels of antimicrobial peptides (AMPs) in atopic dermatitis (AD) have previously been reported and linked to the increased susceptibility skin infections found AD patients. This study intents identify AMPs: hBD-2, hBD-3, RNase7, psoriasin LL-37 patients healthy controls, determine concentrations consecutive depths outer most layers. Tape stripping was used on lesional non-lesional skin. From each site, 35 tape strips were collected pooled groups 5. Commercially available ELISA...
Staphylococcus aureus skin colonization is common in patients with atopic dermatitis (AD) and associated risk of infections. AD therefore often receive antibiotic treatments, including topical treatment fusidic acid, which have been resistance development. To examine the prevalence S. isolated from Danish patients, a primary focus on acid genetic mechanisms that underlie it. One hundred thirty-eight isolates collected lesional (n = 54), non-lesional 27) anterior nares 57) 71 adult were...
Tape-strips are a minimally invasive approach to characterize skin biomarkers in atopic dermatitis (AD). However, they have not yet been used for tracking gene expression changes with systemic treatment.The aim of the study was evaluate and therapeutic response AD patients before after dupilumab (interleukin 4Rα antibody) treatment using tape-strips obtain epidermal tissue analysis.Lesional nonlesional tape-stripped sampled from 18 17 healthy subjects analyzed by RNA-seq.At baseline, we...
Introduction. The pathogenesis of atopic dermatitis (AD) is not yet fully understood, but the bacterial composition AD patients' skin has been shown to have an increased abundance Staphylococcus aureus. More recently, coagulase-negative (CoNS) species were be able inhibit S. aureus, further studies are required determine effects community variation in AD.Aim. Here we investigated whether analysing metabarcoding data with more recently developed DADA2 approach improves analyses compared...
Atopic dermatitis (AD) is a common inflammatory skin disease characterized by an epidermal barrier impairment, as well Th2/Th22-skewed immune response, both favoring colonization with Staphylococcus aureus . Colonization strongly related to severity of the disease, and reduction S. has been found alleviate symptoms. Lactic acid bacteria (LAB) produce antimicrobial compounds such organic acids bacteriocins are widely used probiotics. The aim this study was isolate LAB screen for antibacterial...
Abstract Background Atopic dermatitis (AD) patients have an altered skin bacterial community, with abundance of Staphylococcus aureus associated flares, highlighting that microbial organisms may be important for disease exacerbation. Despite strong evidence association between colonisation and AD, very limited knowledge regarding the eukaryotic including fungi ectoparasites, in AD exists. In this study, we compared nasal community adult ( n = 55) non-AD healthy controls 45) using targeted...
A strong link between disease severity and Staphylococcus aureus colonization of the skin has been reported in patients with atopic dermatitis (AD).To examine temporal variations S. CC type AD, to investigate links severity, barrier properties filaggrin gene (FLG) mutations.This was a follow-up study cohort 101 adult AD recruited from an outpatient clinic. Bacterial swabs were taken at baseline lesional skin, nonlesional nose. Swabs positive for characterized by spa respective clonal complex...
Background While Staphylococcus aureus (S. aureus) colonization has been thoroughly studied in atopic dermatitis (AD), where S. is related to flares and considered a trigger factor, hand eczema (HE) only sparsely studied. Objectives To examine the 1-week prevalence of HE patients, its association with severity, subtype, AD, nasal compared healthy controls. Methods In case-control study 50 adult patients controls, bacterial swabs from lesional skin (patients only), non-lesional (dorsal hand),...