The current study aimed to develop carbomer based hydrogel dressings, incorporating ethanolic extracts of Rosmarinus officinalis aerial parts, Achillea millefolium and Calendula flowers. pharmaceutical properties the obtained hydrogels, as well their texture antimicrobial activity, were further evaluated. Five wound dressing formulations on carbopol prepared. addition formulation slightly lowered pH expected. parts extract loaded proved be firmest one. In terms consistency viscosity,...

Diabetic autonomic neuropathy is probably the most undiagnosed but serious complication of diabetes. The main objectives were to assess prevalence peripheral and in a population diabetic patients, analyze it real-life outpatient unit scenario determine feasibility performing SUDOSCAN tests together with widely used for neuropathy. A total 33 patients included study. Different scoring systems (the Toronto Clinical Neuropathy Score—TCNS; Disability Score—NDS; Symptom Score—NSS) applied record...

The aim of the current study was to investigate prevalence Clostridium perfringens (C. perfringens) recovered from animal faeces, as well determine antimicrobial susceptibility such isolates. A total 14 (14/100; 14%) C. isolates were isolated 100 analysed samples (twelve faecal collected pigs and two veal calves' samples). preponderant genotype type A, with all being cpa-positive. most potent agents against proved be vancomycin, rifampicin lincomycin. strong resistance tetracycline (71.4%),...

The current study was conducted in order to analyze the prevalence of Escherichia coli (E. coli) samples chicken meat (100 samples), as well evaluate antimicrobial susceptibility these isolates. A total 30 were positive for E. among collected samples. Most isolates proved be highly resistant tetracycline (80%), ampicillin sulfamethoxazole (73.33%), chloramphenicol (70%) and nalidixic acid (60%). Strong resistance ciprofloxacin (56.66%), trimethoprim (50%), cefotaxime (46.66%), ceftazidime...

Gastroenteritis attributable to Salmonella enterica and the continuous increase in antimicrobial resistance of this gut pathogen, which compromises use previously effective treatments, is great concern for public health. This study was conducted order investigate presence plasmid-mediated quinolone (PMQR) determinants β-lactamase-encoding genes, S. enterica, isolated from humans, one companion animal food. Moreover, aimed identify potential vehicles transmission resistant strains with focus...

The presence of antibiotic residues in honey has recently become a growing public health concern due to the overuse these products beekeeping and their transfer food industry. aim this study was develop validate simple, selective sensitive method for simultaneous identification quantification twenty-two sulfonamides, trimethoprim dapsone using rapid reliable liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. developed showed good linearity, specificity, precision...

This study evaluates antibiotic residues and bacterial loads in influent effluent samples from three wastewater treatment plants (WWTPs) Romania, across four seasons 2021 to 2022. Analytical methods included solid-phase extraction high-performance liquid chromatography (HPLC) quantify concentrations, while microbiological assays estimated assessed resistance patterns. Statistical analyses explored the impact of environmental factors such as temperature rainfall on levels. The results showed...

Salmonella is considered to be one of the major foodborne pathogens associated with consumption contaminated poultry meat products. To best our knowledge this first extended research performed on a number strains isolated during 2011-2021 from products in Romania. The aim study was characterize prevalence pathogenic serovars, antimicrobial susceptibility, and resistance genes 112 isolates recovered raw results showed that enterica serovars Enteritidis Typhimurium were common serotypes (56%;...

This study describes an innovative in-line near-infrared (NIR) process monitoring method for the quantification of total polyphenolic content (TPC) Ajuga genevensis dry extracts. The extract was obtained in a fluidized bed processor, by spraying and adsorbing liquid onto inert powder support. NIR spectra were recorded continuously during extract's process. For calibration TPC method, samples collected entire each sample assessed spectroscopically, applying UV-Vis reference method. values...

Abstract In this work, FT‐IR absorbance spectra of genomic DNAs isolated from four strains Escherichia coli (A, B, C and D) two Salmonella (E F), respectively, have been analyzed in both untreated UV femtosecond laser pulses irradiated samples, to investigate their screening characteristics structural radiotolerance at 256 nm. Spectroscopic band assignments aspects are discussed the 600–1800 cm −1 3000–4000 spectral ranges. DNA changes level nucleic acids backbone, sugar, PO 2 − group,...

Fresh rainbow trout fillets are very perishable food products.Therefore, they cannot be stored at refrigeration temperatures for a long period of time.High pressure processing (HPP) can improve the quality through microbial load control.As result this, shelf-life product is extended.However, some physicochemical changes appear.The aim this study was to assess degree protein denaturation in treated with various levels high pressure.The results showed that definitely higher levels, range 400...

The variety and multiple dimensions of pain (acute/chronic, mild/moderate/severe, nociceptive/neuropathic) requires different pharmacologic non-pharmacologic treatments in certain patients.A lot topical formulation, from various therapeutic classes have been proposed order to decrease systemic exposure reduce the risks adverse events.Topical as well transdermal drug delivery systems are medicated plasters with: anesthetics (lidocaine), analgesic or nonsteroidal anti-inflamatory drugs...

Silver antimicrobial dressings have been extensively used as therapeutic strategies in wound management. The present study sought to asses and compare the efficacy of commercially available modern silver-wound dressings. activity silver has evaluated by disk diffusion method, including five common pathogens. silver-release profile determined using inductively coupled plasma–mass spectrometry. results demonstrated different degrees against tested is released at rates, within a very large...

Abstract This research was conducted in order to reveal antimicrobial resistance patterns and the prevalence of genes encoding E scherichia coli isolated from raw poultry meat. One hundred samples meat were collected, 51 isolates . recovered further tested for susceptibility 11 antimicrobials, using classical disk diffusion method. Fifteen isolates, showing a high degree among collection examined by multiplex polymerase chain reaction. Resistance most frequently observed tetracycline...

At present, the epidemiology of gastrointestinal disease caused by Clostridioides difficile (C. difficile) is starting to be slowly elucidated internationally, although information about bacteria in food supply chain insufficient and, many countries, even absent. The study was conducted order investigate prevalence C. isolated from animal feces, as well determine antimicrobial susceptibility such isolates. presence antibiotic resistance determinants has also been evaluated. Overall, a total...

ABSTRACT The global growth of antimicrobial resistance (AMR) raises significant challenges to public health, necessitating comprehensive surveillance and intervention strategies. This study investigates the prevalence profiles Escherichia coli isolated from three different wastewater treatment plants (WWTPs) in Romania during warm season 2023. Through systematic sampling subsequent microbiological molecular analyses, we identified a 50% E. samples, with concerning 83.34% isolates displaying...

Antibiotic resistance among pathogenic bacteria in humans and animals poses a critical public health challenge, leading to diminished effectiveness of existing antimicrobial treatments. Notably, animal-derived food products are significant vectors for the transmission resistant humans, with

Vibrational properties and structural behavior upon ultraviolet (UV) irradiation are reported here for DNAs isolated from three serovars of Salmonella bacteria, respectively (S. enteritidis, S. infantis typhimurium) by using Fourier transform infrared spectroscopy (FT-IR). The FT-IR absorbance spectra were recorded bacterial DNAs, control UV irradiated samples (30 min, 1 h, h 30 min), respectively, presented in the wavenumber ranges 800 to 1800 cm−1 3000 4000 cm−1, respectively. Molecular...

The presence of antibiotic residues in honey has recently become a growing public health concern due to the overuse these products beekeeping and their transfer food industry. aim this study was develop validate simple, selective sensitive method for simultaneous identification quantification twenty-two sulfonamides, trimethoprim dapsone using rapid reliable liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. developed showed good linearity, specificity, precision...

Introduction: Escherichia coli is usually a commensal bacterium of humans and animals. Pathogenic variants cause intestinal extra infections, including gastroenteritis, urinary tract infection, meningitis, peritonitis septicemia . Nowadays, the worldwide concern on spread this mainly connected to resistance phenomenon. Aims: This research was conducted in order reveal antimicrobial patterns , causing human illness. Materials methods: A total 30 isolates were recovered from patients...