A5039917069- Electrospun Nanofibers in Biomedical Applications
- Pancreatic function and diabetes
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Nerve injury and regeneration
- Diabetes and associated disorders
- Pluripotent Stem Cells Research
- Cannabis and Cannabinoid Research
- Silk-based biomaterials and applications
- Diabetes Management and Research
- Analytical Chemistry and Sensors
- Diet and metabolism studies
- Periodontal Regeneration and Treatments
- Spaceflight effects on biology
- Cellular Mechanics and Interactions
- Graphene and Nanomaterials Applications
- Hemodynamic Monitoring and Therapy
- Birth, Development, and Health
- Autophagy in Disease and Therapy
- Cardiac, Anesthesia and Surgical Outcomes
- Wireless Power Transfer Systems
- Infectious Aortic and Vascular Conditions
- Renal and Vascular Pathologies
Politecnico di Milano
2017-2020
Mario Negri Institute for Pharmacological Research
2013-2019
Istituti di Ricovero e Cura a Carattere Scientifico
2013-2019
For chronic kidney diseases, there is little chance that the vast majority of world's population will have access to renal replacement therapy with dialysis or transplantation. Tissue engineering would help address this shortcoming by regeneration damaged using naturally occurring scaffolds seeded precursor cells. The aims present study were optimize production three-dimensional (3D) rat whole-kidney shortening duration organ decellularization process detergents avoid nonionic compounds,...
Abstract The rising number of patients needing renal replacement therapy, alongside the significant clinical and economic limitations current therapies, creates an imperative need for new strategies to treat kidney diseases. Kidney bioengineering through production acellular scaffolds recellularization with stem cells is one potential strategy. While protocols obtaining organ have been developed successfully, scaffold more challenging. We evaluated in vivo vitro procedures. Our results show...
Abstract Generating new kidneys using tissue engineering technologies is an innovative strategy for overcoming the shortage of donor organs transplantation. Here we report how to efficiently engineer kidney vasculature decellularized rat scaffolds by human induced pluripotent stem cell (hiPSCs)-derived endothelial cells (hiPSC-ECs). In vitro , hiPSC-ECs responded flow stress acquiring alignment orientation, and attached proliferated on acellular sections, maintaining their phenotype. The...
The clinical usability of pancreatic islet transplantation for the treatment type I diabetes, despite some encouraging results, is currently hampered by short lifespan transplanted tissue. In vivo studies have demonstrated that co-transplantation Mesenchymal Stem Cells (MSCs) with islets more effective respect to alone in ensuring glycemia control diabetic rats, but molecular mechanisms this action are still unclear. aim study was elucidate positive effect MSCs on functionality setting up...
Stem cell fate and behavior are affected by the bidirectional communication of cells their local microenvironment (the stem niche), which includes biochemical cues, as well physical mechanical factors. normally cultured in conventional two-dimensional monolayer, with a environment very different from physiological one. Here, we compare culture rat mesenchymal on flat supports “Nichoid”, an innovative three-dimensional substrate micro-engineered to recapitulate architecture niche vitro. Two...
Chronic renal insufficiency inexorably progresses in patients, such as it does after partial ablation rats. However, the progression of diseases can be delayed by angiotensin II blockers that stabilize function or increase GFR, even advanced phases disease. Regression glomerulosclerosis induced antagonism, but effect these treatments on entire vascular tree is unclear. Here, using microcomputed tomography and scanning electron microscopy, we compared size extension kidney blood vessels...
Abstract Background The use of pluripotent cells in stem cell therapy has major limitations, mainly related to the high costs and risks exogenous conditioning feeder layers during expansion passages. Methods We developed an innovative three-dimensional culture substrate made “nichoid” microstructures, nanoengineered via two-photon laser polymerization. nichoids limit dimension adhering embryoid bodies expansion, by counteracting migration between adjacent units its microarchitecture....
Clinically available alternatives of vascular access for long-term haemodialysis-currently limited to native arteriovenous fistulae and synthetic grafts-suffer from several drawbacks are associated high failure rates. Bioprosthetic grafts tissue-engineered blood vessels costly without clearly demonstrated increased performance. In situ tissue engineering could be the ideal approach provide a that profits advantages in short-term (e.g. early cannulation) success rates driven by...
This work deals with the development and characterization of a potentially implantable blood pressure telemetry system, based on an active Radio-Frequency IDentification (RFID) tag, International Organization for Standardization (ISO) 15693 compliant. approach aims to continuously measure average, systolic diastolic small/medium animals. The measured wave undergoes embedded processing results are stored onboard in non-volatile memory, providing data under interrogation by external RFID...
This work deals with the development of an implantable blood pressure telemetry system, based on active RFID tag, ISO 15693 compliant, aimed to continuously measure average, systolic and diastolic pressure. The wave undergoes embedded processing results are stored onboard in a non-volatile memory, providing data under interrogation by external reader. paper presents experimental characterization laboratory preliminary in-vivo tests. device is mainly intended for monitoring freely moving test...
Transplantation of pancreatic islets is an intriguing new therapeutic option to face the worldwide spread problem Type-I diabetes. Currently, its clinical use limited by several problems, mainly based on high number required restore normoglycaemia and low survival transplanted tissue. A promising attempt overcome limits such approach was represented Mesenchymal Stem Cells (MSC). Despite encouraging results obtained with murine-derived MSC, little still known about their protective...
Transplantation of pancreatic islets has become a promising clinical option to treat patients with type 1 diabetes, alternative the standard therapy insulin injections. Islet transplantation is minimally invasive therapeutic approach, and it allows better metabolic control long-term independence in more than 80% (Ryan et al., 2002). However this treatment some side effects, such as poor yield islet explants even immune graft rejection, which have consequence very limited lifespan...
The total mass of pancreatic islet cells is a critical factor in glucose metabolic control. aim the present study was to investigate whether Munich Wistar Frömter (MWF) rat, beside reduction number nephrons, there are also alterations islets and β cell mass. We examined metabolism, both normal conditions following intravenous injection. per pancreas, estimated by morphometrical analysis, significantly lower MWF rats than (3,501±1,285 vs. 7,259±2,330 islet/rat, respectively). Also mean gram...