A5065268908- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Genomics and Phylogenetic Studies
- Marine Toxins and Detection Methods
- Nanoparticle-Based Drug Delivery
- Diatoms and Algae Research
- Free Radicals and Antioxidants
- Marine and coastal plant biology
- Biocrusts and Microbial Ecology
- Marine Biology and Ecology Research
- Bacterial Genetics and Biotechnology
- Electron Spin Resonance Studies
- Phytase and its Applications
- Mollusks and Parasites Studies
- Advanced Drug Delivery Systems
- Plant nutrient uptake and metabolism
- Metal-Catalyzed Oxygenation Mechanisms
- Graphene and Nanomaterials Applications
- Plant biochemistry and biosynthesis
- Light effects on plants
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Fermentation and Sensory Analysis
Kobe University
2022-2024
National Institute of Genetics
2017-2023
Hokkaido University
2013-2020
Tohoku University
2015-2020
Dynamic Research (United States)
2015
Yamagata University
2014
Braarudosphaera bigelowii (Prymnesiophyceae) is a coastal coccolithophore with long fossil record, extending back to the late Cretaceous (ca. 100 Ma). A recent study revealed close phylogenetic relationships between B. bigelowii, Chrysochromulina parkeae (Prymnesiophyceae), and prymnesiophyte that forms symbiotic association nitrogen-fixing cyanobacterium UCYN-A. In order further examine these relationships, we conducted transmission electron microscopic molecular studies of bigelowii. TEM...
Significance Extremely acidic environments are scattered worldwide, and their ecosystems supported by acidophilic microalgae as primary producers. To understand how algae evolved from respective neutrophilic ancestors, we determined the draft genome sequence of green alga Chlamydomonas eustigma performed comparative analyses between C . its relative reinhardtii The results suggest that higher expression heat-shock proteins H + -ATPase, loss some metabolic pathways acidify cytosol,...
Sexual reproduction is widespread in eukaryotes; however, only asexual has been observed unicellular red algae, including Galdieria , which branched early Archaeplastida. possesses a small genome; it polyextremophile, grows either photoautotrophically, mixotrophically, or heterotrophically, and being developed as an industrial source of vitamins pigments because its high biomass productivity. Here, we show that exhibits sexual life cycle, alternating between cell-walled diploid cell...
Abstract A monophyletic group of dinoflagellates, called ‘dinotoms’, are known to possess evolutionarily intermediate plastids derived from diatoms. The diatoms maintain their nuclei, mitochondria, and the endoplasmic reticulum in addition with plastids, while it has been observed that host dinoflagellates retain permanently by controlling diatom karyokinesis. Previously, we showed dinotoms have repeatedly replaced Here, show process replacements is at two different evolutionary stages...
Skin lesions, cataracts, and congenital anomalies have been frequently associated with inherited deficiencies in enzymes that synthesize cholesterol. Lanosterol synthase (LSS) converts (S)-2,3-epoxysqualene to lanosterol the cholesterol biosynthesis pathway. Biallelic mutations LSS reported families cataracts and, very recently, cases of hypotrichosis. However, it remains be clarified whether these phenotypes are caused by enzymatic each tissue, disruption activity vivo has not yet...
Secondary loss of photosynthesis is observed across almost all plastid-bearing branches the eukaryotic tree life. However, genome-based insights into transition from a phototroph secondary heterotroph have so far only been revealed for parasitic species. Free-living organisms can yield unique evolutionary consequence photosynthesis, as lifestyle requires specific adaptations to host environments. Here, we report on diploid genome free-living diatom Nitzschia putrida (35 Mbp),...
Free radical scavenging activities of two series tripeptide libraries were investigated using ABTS, DPPH, and ORAC assays. Two Tyr-containing tripeptides showed higher against the hydrophilic ABTS AAPH radicals than His-containing tripeptides, showing that Tyr residues play important roles in free activity. Higher concentrations required to reveal apparent DPPH The antioxidant obtained from this study compared with previous results several assays, including activity peroxidation linoleic...
Metabolism, cell cycle stages, and related transcriptomes in eukaryotic algae change with the diel of light availability. In unicellular red alga Cyanidioschyzon merolae, S M phases occur at night. To examine how transcriptomic changes metabolic pathways are to identify all genes for which mRNA levels depending on cycle, we examined C. merolae. addition, compared between wild type transgenic lines, was uncoupled from by depletion either cyclin-dependent kinase A or retinoblastoma-related...
Replication of the circular bacterial chromosome is initiated at a unique origin (oriC) in DnaA-dependent manner which replication proceeds bidirectionally from oriC to ter. The nucleotide compositions most bacteria differ between leading and lagging DNA strands. Thus, chromosomal sequence typically exhibits an asymmetric GC skew profile. Further, free-living without genomes encoding dnaA were unknown. DnaA-oriC-dependent initiation mechanism may be essential for bacteria. However,...
Abstract Feeding on unicellular photosynthetic organisms by eukaryotes is the base of aquatic food chain and evolutionarily led to establishment endosymbionts/organelles. Photosynthesis generates reactive oxygen species damages cells; thus, possess several mechanisms cope with stress. Here, we demonstrate that prey also exposes amoebozoan excavates predators oxidative Upon illumination, there a commonality in transcriptomic changes among distant feeding prey. One genes commonly upregulated...
Abstract Endosymbiosis of unicellular eukaryotic algae into previously nonphotosynthetic eukaryotes has established chloroplasts in several lineages. In addition, certain organisms different lineages ingest and utilize them as temporal (kleptoplasts) for weeks to months before digesting them. Among these organisms, the dinoflagellate Nusuttodinium aeruginosum ingests cryptomonad Chroomonas sp. enlarges kleptoplast with aid nucleus. To understand how nucleus is remodeled dinoflagellate, here...
Dinothrix paradoxa and Gymnodinium quadrilobatum are benthic dinoflagellates possessing diatom-derived tertiary plastids, so-called dinotoms. Due to the lack of available genetic information, their phylogenetic relationships remain unknown. In this study, sequencing 18S rDNA rbcL gene from temporary cultures isolated natural samples revealed that they close relatives another dinotom, Galeidinium rugatum. The morphologies these three dinotoms differ significantly each other; however, share a...
:Nusuttodinium spp. use ingested cryptomonad chloroplasts as kleptochloroplasts but form a sister group to Spiniferodinium spp., which are typical photosynthetic dinoflagellates. Our survey of the diversity Nusuttodinium-related dinoflagellates resulted in discovery two colourless dinoflagellates, referred DAI (dinoflagellate collected from Aininkappu) and DIS Ishikari). Cells possess right-handed cingulum, an apical groove connecting with sulcal extension at right angle nuclear chambers....
The order Trypanosomatida has been well studied due to its pathogenicity and the unique biology of mitochondrion. In Trypanosoma brucei, four DNA polymerases, namely PolIA, PolIB, PolIC, PolID, related bacterial polymerase I (PolI), were shown be localized in mitochondria experimentally. These mitochondrion-localized polymerases are phylogenetically distinct from other family A such as PolI, gamma (Polγ) human yeasts, "plant protist organellar (POP)" diverse eukaryotes. However, diversity...
Abstract The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which red algal endosymbiont was integrated into previously nonphotosynthetic eukaryote. retain remnant the nucleus (nucleomorph) that is replicated once cell cycle along with chloroplast. To understand how chloroplast, nucleomorph and host divide coordinated manner, we examined expression genes/proteins are related to replication chloroplast division as well timing nuclear DNA synthesis cryptophyte...
Purpose: Biodegradable nanoparticles (NPs) composed of poly(D, L-lactide-co-glycolide) (PLGA) have attracted considerable attention as delivery systems drugs and antioxidative compounds, such β-carotene (BC). Intravenous (IV) administration BC-containing PLGA-NPs (BC-PLGA-NPs) coated with polysorbate 80 (PS80) has been shown to effectively deliver BC the brain. However, whole-body distribution profile is still not clear. Therefore, we investigated accumulation in various organs, including...
While photoautotrophic organisms utilize inorganic nitrogen as the source, heterotrophic organic and thus do not generally have an assimilation pathway. Here, we focused on metabolism of Rapaza viridis, a unicellular eukaryote exhibiting kleptoplasty. Although belonging to lineage essentially flagellates, R. viridis exploits photosynthetic products kleptoplasts was therefore suspected potentially nitrogen. From transcriptome data identified gene RvNaRL, which had sequence similarity genes...
:A new dinoflagellate, Paragymnodinium stigmaticum Yokouchi, Onuma & Horiguchi sp. nov., was described from sand samples collected at Sumiyoshi beach, Sado Island, Niigata Prefecture, Japan, based on observations the light, scanning and transmission electron microscope levels together with molecular characterisation. The cells were 8.5–15.2 μm long 6.3–12.4 wide. episome hyposome subequal hemispherical; although, could also be conical. cingulum wide, well excavated descended one quarter to a...
The species Amphidinium eludens, as described by Herdman (1922; Proc. Trans. Liverpool Biol. Soc. 36: 18) based on her drawing in fig. 1, has been investigated for the first time scanning electron microscopy and phylogenetic analysis. morphological molecular data reveal that this is distantly related to other known dinoflagellates. Balech [1956, Rev. Algol., n. ser. 2(1–2): 30] cited eludens (1922, 1) basionym of type Adenoides, while he illustrated kofoidii 2) Adenoides eludens. As...
Abstract Photosymbioses, the symbiotic relationships between photosynthetic algal symbionts and non-photosynthetic eukaryotic hosts, are sporadically found in a lot of lineages, but only few taxa, such as cnidarians ciliates hosting endosymbionts, have been actively studied for long time. That has hindered understanding universal mechanisms photosymbiosis establishment. Especially Amoebozoa, two species, Mayorella viridis Parachaos zoochlorella , reported photosymbiotic nature, their...