A5014288672- Epigenetics and DNA Methylation
- Developmental Biology and Gene Regulation
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Estrogen and related hormone effects
- Histone Deacetylase Inhibitors Research
- Hedgehog Signaling Pathway Studies
- Sirtuins and Resveratrol in Medicine
- CRISPR and Genetic Engineering
- Synthesis and Characterization of Heterocyclic Compounds
- RNA Research and Splicing
- Cancer-related gene regulation
- Click Chemistry and Applications
- Gene Regulatory Network Analysis
- MicroRNA in disease regulation
- RNA modifications and cancer
- TGF-β signaling in diseases
- Erythrocyte Function and Pathophysiology
- Ubiquitin and proteasome pathways
- Hippo pathway signaling and YAP/TAZ
- Light effects on plants
- Receptor Mechanisms and Signaling
- Phagocytosis and Immune Regulation
- Adenosine and Purinergic Signaling
- Quinazolinone synthesis and applications
Medical University of Lublin
2018-2025
Sirtuin 7 (SIRT7), a member of the sirtuin family NAD+-dependent deacetylases, plays vital role in cancer, exhibiting context-dependent functions across various malignancies. Our study investigates SIRT7 depletion head and neck squamous cell carcinoma (HNSCC) progression. In vitro 3D organotypic models demonstrated that knock-out attenuates cancer viability, proliferation, motility as well induces downregulation migration- epithelial-mesenchymal transition (EMT)-related gene expression....
Colorectal cancer (CRC) is the third leading cause of cancer-related deaths in men and women. The impact new pyrazolo[4,3-e]tetrazolo[1,5-b][1,2,4]triazine sulphonamide (MM-129) was evaluated against human colon vitro zebrafish xenografts. Our results show that this synthesised compound effectively inhibits cell survival BTK-dependent mechanism. Its effectiveness much higher at a relatively low concentration as compared with standard chemotherapy used for CRC, i.e. 5-fluorouracil (5-FU)....
The Notch signaling pathway is a crucial regulator of cell differentiation as well tissue organization, whose deregulation linked to the pathogenesis different diseases. NOTCH1 plays key role in breast cancer progression by increasing proliferation, maintenance stem cells, and impairment death. mechanosensitive receptor, where mechanical force required activate proteolytic cleavage release intracellular domain (NICD). We circumvent this limitation regulating activity light. To achieve this,...
Notch signalling is one of the key molecular pathways involved in cell-to-cell signal transduction. Although mechanisms action NOTCH receptors are already relatively well known, their biological implications remain unclear, especially during initiation and progression head neck squamous cell carcinoma (HNSCC). Here, we present growth- differentiation-modulating effects various "next generation" small molecule modulators represented by RIN-1, CB-103, on HNSCC, compared to gamma secretase...
Synthetic biology involves the engineering of logic circuit gates that process different inputs to produce specific outputs, enabling creation or control biological functions. While CRISPR has become tool choice in molecular due its RNA-guided targetability other nucleic acids, it not been frequently applied beyond those controlling guide RNA (gRNA). In this study, we present an adaptation split Cas9 generate capable sensing events, leveraging a reporter (EGxxFP) detect occurrences such as...
Recombinant human erythropoietin (Epo) is an effective and convenient treatment for cancer-related anaemia. In our study the first time, we evaluated effect of simultaneous use Epo Bruton's tyrosine kinase (BTK) inhibitor LFM-A13 on viability tumour development breast cancer cells. The results demonstrated that significantly intensifies anticancer activity in MCF-7 MDA-MB-231. featured therapeutic scheme efficiently blocked zebrafish experimental model. administered together resulted cell...
Aberration of Notch signaling is one the key events involved in development and progression head neck squamous cell carcinoma (HNSCC). The pathway controls tissue-specific differentiation normal epithelial cells frequently altered carcinomas, thus affecting their proliferation, growth, survival, chemosensitivity or resistance against anti-cancer agents. In this study, we show that use novel, small-molecule inhibitors signaling, such as FLI-06, can have a beneficial effect on increasing HNSCC...
<title>Abstract</title> Sirtuin 7 (SIRT7), a member of the sirtuin family NAD+-dependent deacetylases, plays vital role in cancer, exhibiting context-dependent functions across various malignancies. Our study investigates SIRT7 depletion head and neck squamous cell carcinoma (HNSCC) progression. <italic>In vitro</italic> 3D organotypic models demonstrated that knock-out attenuates cancer viability, proliferation, motility as well induces downregulation migration- epithelial-mesenchymal...
TWIST1 is a transcription factor that affects cell behavior during development and differentiation. Yet, it better known for its roles in neoplasia through regulation of plasticity. The pathological contributions tumor initiation, angiogenesis, invasion, metastasis, chemo-resistance have been the focus much research. To-date, only way to quantitatively measure abundance TWIST by immunoblots. no bioassay exists can detect activity. Thus, we present here cell-based assay allows measuring...
Abstract The Notch signalling pathway is a crucial regulator of cell differentiation as well tissue organisation. Dysregulation has been linked to the pathogenesis different diseases. plays key role in breast cancer progression by controlling interaction between tumour cells and microenvironment increasing motility invasion. NOTCH1 mechanosensitive receptor, where mechanical force required activate proteolytic cleavage release intracellular domain (NICD). Here, we circumvent this step...
Synthetic biology involves the generation of logic circuits to create or control biological functions and behaviours by engineering interconnected genetic elements such as promoters, repressors, transcriptional activators. CRISPR discovery, its adaptations mammalian cells, has made it tool choice in molecular revolutionized genome biomedical sciences. Here, we describe an adaptation a split Cas9 generate synthetic gates sense events. As proof-of-concept, complementing halves were placed...
ABSTRACT Cell signalling pathways, in particular downstream receptor activation, frequently converge the activation of transcriptional modulators. Yet, cells are able to differentiate stimulation each receptor. It has become clear that modulators, such as transcription factors, do not work on or off states but rather patterns active/inactivate conformations. Thus, it is intensity and duration fluctuating activity result differential cellular genes expression changes, this challenging...
(1) Background: The purpose of the given study was to examine antitumor activity simultaneous administration MM-129, a 1,2,4-triazine derivative, and indoximod (IND), kynurenine pathway inhibitor, toward colon cancer. (2) Methods: efficiency co-administration studied compounds assessed in xenografted zebrafish embryos. Then, effects combined on cellular processes such as cell viability, apoptosis, intracellular signaling pathways were evaluated. In vitro studies performed using two...