A5007717169- Plant and Biological Electrophysiology Studies
- Photoreceptor and optogenetics research
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Cancer-related molecular mechanisms research
- Protease and Inhibitor Mechanisms
- Ubiquitin and proteasome pathways
- Calcium signaling and nucleotide metabolism
- Chromosomal and Genetic Variations
- Colorectal Cancer Treatments and Studies
- MicroRNA in disease regulation
- Bone health and treatments
- Plant Molecular Biology Research
- Ion channel regulation and function
- S100 Proteins and Annexins
- DNA and Nucleic Acid Chemistry
- Parasitic Infections and Diagnostics
- Apelin-related biomedical research
- Plant Stress Responses and Tolerance
- Psoriasis: Treatment and Pathogenesis
- Heme Oxygenase-1 and Carbon Monoxide
- Calpain Protease Function and Regulation
- Legume Nitrogen Fixing Symbiosis
- Oral microbiology and periodontitis research
- Axon Guidance and Neuronal Signaling
Zhuhai People's Hospital
2013-2024
Jinan University
2015-2024
Zhengzhou University
2023
Vienna Biocenter
2023
University of Würzburg
2019-2022
Hunan Cancer Hospital
2018
Central South University
2013-2018
Sun Yat-sen University
2015-2016
Sun Yat-sen University Cancer Center
2015-2016
State Key Laboratory of Oncology in South China
2015
A novel cDNA, IA-2beta, was isolated from a mouse neonatal brain library. The predicted protein sequence revealed an extracellular domain, transmembrane region, and intracellular domain. domain is 376 amino acids long 74% identical to the of IA-2, major autoantigen in insulin-dependent diabetes mellitus (IDDM). partial IA-2beta indicates that it differs substantially (only 26% identical) IA-2. Both molecules are expressed islets tissue. Forty-six percent (23 50) IDDM sera but none normal...
Replication Protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein (SSB) found in all eukaryotic cells. RPA known to be required for many of the same reactions catalyzed by homotetrameric SSB bacteria, but its origin, subunit functions, and mechanism binding remain mystery. Here we show that three subunits yeast contain total four domains with weak sequence similarity Escherichia coli protomer. We refer these regions as potential ssDNA-binding (SBDs). The p69 subunit, which...
Abstract In contrast to the plasma membrane, vacuole membrane has not yet been associated with electrical excitation of plants. Here, we show that mesophyll vacuoles from Arabidopsis sense and control potential essentially via K + -permeable TPC1 TPK channels. Electrical stimuli elicit transient depolarization can last for seconds. excitability is suppressed by increased vacuolar Ca 2+ levels. comparison wild type, fou2 mutant, harboring channels insensitive luminal , be excited fully even...
Significance Despite decades of biophysical and structural research, little is understood about how voltage-gated ion channels (VGICs) activate during membrane depolarization, less known VGICs can be modulated by lipids other ligands. We identify multiple functional states the voltage- Ca 2+ -gated channel TPC1 from Arabidopsis thaliana (AtTPC1), which confers electrical excitability to plant vacuole. Here, we show a voltage-sensing domain (VSD) functions activation mechanism inhibition...
Abstract The regulation of stability is particularly crucial for unstable proteins in cells. However, a convenient and unbiased method identifying regulators protein remains to be developed. Recently, genome-scale CRISPR-Cas9 library has been established as genetic tool mediate loss-of-function screening. Here, we developed screening assay (Pro-SRSA) by combining the whole-genome with dual-fluorescence-based reporter high-throughput sequencing screen stability. Using Cdc25A an example,...
The diagnostic and prognostic significance of increased cathepsin B (CTSB) D (CTSD) concentration in the serum cancer patients were evaluated for some tumor types. High expression CTSD CTSB was detected biopsy tissues from nasopharyngeal carcinoma (NPC). However, whether serve as markers NPC remains unclear.Serum samples collected 40 healthy volunteers 80 enrolled study. using enzyme-linked immunosorbent assay (ELISA). Concomitantly, relationship between concentrations clinicopathological...
Human single-stranded DNA binding protein 1 (hSSB1) plays a critical role in responding to damage and maintaining genome stability. However, the regulation of hSSB1 remains poorly studied. Here, we determined that acetylation at K94 mediated by acetyltransferase p300 deacetylases SIRT4 HDAC10 impaired its ubiquitin-mediated degradation proteasomes. Moreover, demonstrated hSSB1-K94R mutant had reduced cell survival response radiation or chemotherapy drugs. Furthermore, p300/CBP inhibitor C646...
Nasopharyngeal carcinoma (NPC) has a high metastatic clinicopathological feature. As carcinogen factor, N,N'-dinitrosopiperazine (DNP) is involved in NPC metastasis, but its precise mechanism not been fully elucidated. Herein, we showed that DNP promotes metastasis through upregulating miR-149. was found to decrease Plakophilin3 (PKP3) expression, further DNP-decreased PKP3 verified be We also induced proliferation, adhesion, migration and invasion of cell, which inhibited by...
To fire action-potential-like electrical signals, the vacuole membrane requires two-pore channel TPC1, formerly called SV channel. The TPC1/SV functions as a depolarization-stimulated, non-selective cation that is inhibited by luminal Ca 2+ . In our search for species-dependent functional TPC1 variants with different sensitivity, we found in total three acidic residues present sensor sites 2 and 3 of -sensitive AtTPC1 from Arabidopsis thaliana were neutral its Vicia faba ortholog also those...
Aldo‑keto reductase family 1 member B10 (AKR1B10) is a potential marker of several types cancer; however, the role AKR1B10 in nasopharyngeal carcinoma (NPC) remains unclear. In present study, RNA‑seq data and clinical information were obtained from The Cancer Genome Atlas head neck squamous cell (HNSCC) database to evaluate HNSCC. There was no statistically significant difference expression between HNSCC tissues adjacent normal tissues, high not associated with poor overall survival...
Abstract To fire action-potential-like electrical signals, the vacuole membrane requires depolarization-activated two-pore channel TPC1, also called S lowly activating V acuolar SV channel. The TPC1/SV channel, encoded by TPC1 gene, functions as a voltage-dependent and Ca 2+ -regulated potassium currents are activated rise in cytoplasmic but inhibited luminal . In search for species-dependent functional variants, we studied polymorphic amino acids contributing to sensitivity. We found that...
Genetic relationships among 42 grape accession of at least 15 species were estimated and compared using RAPD isozyme techniques. These accessions either hybrids or wild collections the Asiatic species, American European ( V. vinifera ), muscadine rotundifolia ). A total 196 fragments generated from twenty 10-mer primers. The pairwise similarities ranged 0.46 to 0.94. dendrogram was based on similarity coefficients. Species/accessions basically grouped together in accordance with their...