Meghna Gupta
A5061137960- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- Nanoplatforms for cancer theranostics
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- Drug Transport and Resistance Mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Ion channel regulation and function
- Plant nutrient uptake and metabolism
- Advanced Electron Microscopy Techniques and Applications
- Fungal and yeast genetics research
- Innovations in Medical Education
- Endoplasmic Reticulum Stress and Disease
- Protein Tyrosine Phosphatases
- Protein Degradation and Inhibitors
- Biotin and Related Studies
- interferon and immune responses
- Synthesis and Biological Evaluation
- SARS-CoV-2 detection and testing
- Adenosine and Purinergic Signaling
- Multiple Sclerosis Research Studies
- Tuberculosis Research and Epidemiology
- Molecular Communication and Nanonetworks
University of California, San Francisco
2017-2025
Oregon Health & Science University
2024-2025
Creative Commons
2023
All India Institute of Medical Sciences Raipur
2023
All India Institute of Medical Sciences
2023
Central University of Punjab
2023
All India Institute of Medical Sciences Bhopal
2023
Quantitative BioSciences
2020-2022
Shree Guru Gobind Singh Tricentenary University
2022
Jawaharlal Nehru University
1995-2021
The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a grave threat to public health and the global economy. SARS-CoV-2 closely related more lethal but less transmissible coronaviruses SARS-CoV-1 Middle East (MERS-CoV). Here, we have carried out comparative viral-human protein-protein interaction viral protein localization analyses for all three viruses. Subsequent functional genetic screening identified host factors that functionally impinge on...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus enters host cells via an interaction between its Spike protein and the cell receptor angiotensin-converting enzyme (ACE2). By screening a yeast surface-displayed library of synthetic nanobody sequences, we developed nanobodies that disrupt ACE2. Cryo-electron microscopy (cryo-EM) revealed one nanobody, Nb6, binds in fully inactive conformation with binding domains locked into their inaccessible down state, incapable...
Abstract The SARS-CoV-2 protein Nsp2 has been implicated in a wide range of viral processes, but its exact functions, and the structural basis those remain unknown. Here, we report an atomic model for full-length obtained by combining cryo-electron microscopy with deep learning-based structure prediction from AlphaFold2. resulting reveals highly-conserved zinc ion-binding site, suggesting role RNA binding. Mapping emerging mutations variants on shows potential host-Nsp2 interaction regions....
Neuromyelitis optica (NMO) is an autoimmune disease of the central nervous system where pathogenic autoantibodies target water channel aquaporin-4 on human astrocytes causing neurological impairment. Autoantibody binding leads to complement-dependent and complement-independent cytotoxicity, ultimately resulting in astrocyte death, demyelination, neuronal loss. Aquaporin-4 assembles plasma membranes as symmetric tetramers or arrays tetramers. We report molecular structures alone bound Fab...
The unfolded protein response (UPR) detects and restores deficits in the endoplasmic reticulum (ER) folding capacity. Ceapins specifically inhibit UPR sensor ATF6α, an ER-tethered transcription factor, by retaining it at ER through unknown mechanism. Our genome-wide CRISPR interference (CRISPRi) screen reveals that function is completely dependent on ABCD3 peroxisomal transporter. Proteomics studies establish physically associates with ER-resident ATF6α cells vitro a Ceapin-dependent manner....
ATP-binding cassette (ABC) transporters help export various substrates across the cell membrane and significantly contribute to drug resistance. However, a recent study reported an unusual case in which loss of ABC transporter Candida albicans, orf19.4531 (previously named ROA1), increases resistance against antifungal azoles, was attributed altered potential mutant strain. To obtain further mechanistic insights into this phenomenon, here we confirmed that plasma membrane–localized (renamed...
ABSTRACT Without an effective prophylactic solution, infections from SARS-CoV-2 continue to rise worldwide with devastating health and economic costs. gains entry into host cells via interaction between its Spike protein the cell receptor angiotensin converting enzyme 2 (ACE2). Disruption of this confers potent neutralization viral entry, providing avenue for vaccine design therapeutic antibodies. Here, we develop single-domain antibodies (nanobodies) that potently disrupt ACE2. By screening...
Inhibitors of bromodomain and extraterminal domain (BET) proteins are possible anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) prophylactics as they downregulate angiotensin-converting enzyme (ACE2). Here we show that BET should not be inactivated therapeutically because critical antiviral factors at the post-entry level. Depletion BRD3 or BRD4 in cells overexpressing ACE2 exacerbates SARS-CoV-2 infection; same is observed when with endogenous expression treated inhibitors...
Urate provides ~50% of the reducing potential in human and primate plasma which is key to detoxifying reactive oxygen by-products cellular metabolism. endpoint purine metabolism primates, its concentration a balance between excretion from kidney intestine, subsequent reabsorption through cells proximal tubules maintain regulated plasma. SLC2A9 primary transporter that returns urate basolateral side tubule back A shorter splice variant directed apical surface where several transporters...
In the present era of 21st century, apartments and duplex housing play a dominant role in urban settlements. This way settlements has certain benefits, compared to traditional Indian like security, improved quality life, access basic luxury facilities etc. Though this settlement its limitations terms connecting with nature due limited open space availability. Kitchen gardening helps establish direct connection along several other benefits.
Significance Despite decades of biophysical and structural research, little is understood about how voltage-gated ion channels (VGICs) activate during membrane depolarization, less known VGICs can be modulated by lipids other ligands. We identify multiple functional states the voltage- Ca 2+ -gated channel TPC1 from Arabidopsis thaliana (AtTPC1), which confers electrical excitability to plant vacuole. Here, we show a voltage-sensing domain (VSD) functions activation mechanism inhibition...
Target alteration and overproduction drug efflux through overexpression of multidrug transporters localized in the plasma membrane represent conventional mechanisms azole antifungal resistance. Here, we identify a novel conserved mechanism resistance not only budding yeast Saccharomyces cerevisiae but also pathogenic Candida albicans We observed that vacuolar-membrane-localized, protein (MRP) subfamily, ATP-binding cassette (ABC) transporter S. cerevisiae, Ybt1, could import azoles into...
Phosphate is an indispensable metabolite in a wide variety of cells and involved nucleotide lipid synthesis, signaling, chemical energy storage. Proton-coupled phosphate transporters within the major facilitator family are crucial for uptake plants fungi. Similar proton-coupled have been found different protozoan parasites that cause human diseases, breast cancer with elevated demand, osteoclast-like during bone reabsorption, intestinal Caco2BBE homeostasis. However, mechanism proton-driven...
The SARS-CoV-2 protein Nsp2 has been implicated in a wide range of viral processes, but its exact functions, and the structural basis those remain unknown. Here, we report an atomic model for full-length obtained by combining cryo-electron microscopy with deep learning-based structure prediction from AlphaFold2. resulting reveals highly-conserved zinc ion-binding site, suggesting role RNA binding. Mapping emerging mutations variants on shows potential host-Nsp2 interaction regions. Using...
Active DNA-dependent ATPase A Domain (ADAAD) is a SWI2/SNF2 protein that hydrolyzes ATP in the presence of stem-loop DNA contains both double-stranded and single-stranded regions. ADAAD possesses seven helicase motifs are characteristic feature all proteins present yeast as well mammalian cells. In addition, these also possess Q motif ~17 nucleotides upstream I. Using site-directed mutagenesis, we have sought to define role I hydrolysis mediated by ADAAD. We show required for catalysis but...
Plants obtain nutrients from the soil via transmembrane transporters and channels in their root hairs, which ions radially transport toward xylem for distribution across plant body. We determined structures of hyperpolarization-activated channel AKT1 Arabidopsis thaliana, mediates K+ uptake into roots. These AtAKT1 embedded lipid nanodiscs show that undergoes a reduction C4 to C2 symmetry, possibly regulate its electrical activation.
Dear Editor, Pregabalin is a GABA analog with anticonvulsant, analgesic, euphoric, and antianxiety effects.[1] It commonly prescribed for disorders like partial-onset seizures, neuropathic pain, anxiety, fibromyalgia. emerging as potential drug of abuse in India, which very concerning North especially Punjab.[2] There growing evidence from around the world that pregabalin use has surged recently. Previous literature highlighted young male substance disorder, those taking opioids, are at...
A broad chemical genetics screen in