A5021597189- Connexins and lens biology
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Neonatal and fetal brain pathology
- Advanced Breast Cancer Therapies
- Genetic Syndromes and Imprinting
- Breast Cancer Treatment Studies
- Heat shock proteins research
- Anesthesia and Neurotoxicity Research
- Cancer Genomics and Diagnostics
- Cardiovascular Effects of Exercise
- RNA regulation and disease
- Pancreatic function and diabetes
- Evolution and Genetic Dynamics
- Metabolism and Genetic Disorders
- Bacterial Identification and Susceptibility Testing
- Cancer Treatment and Pharmacology
- Kruppel-like factors research
- Biochemical effects in animals
- Diabetes Treatment and Management
- Mitochondrial Function and Pathology
- Hearing, Cochlea, Tinnitus, Genetics
Epigenomics (Germany)
2005-2023
University Children's Hospital Tübingen
2023
Mount Sinai Hospital
2022
University of Toronto
2022
KU Leuven
2022
British Columbia Children's Hospital
2022
Hospital for Sick Children
2022
SickKids Foundation
2022
Europe Hospitals
2022
Curetis (Germany)
2016-2019
ABSTRACT In order to reveal the biological function(s) of gap-junction protein connexin 45 (Cx45), we generated Cx45-deficient mice with targeted replacement Cx45-coding region lacZ reporter gene. Heterozygous Cx45+/− showed strong expression gene in vascular and visceral smooth muscle cells. embryos exhibited striking abnormalities development died between embryonic day (E) 9.5 10.5. Differentiation positioning endothelial cells appeared be normal, but subsequent blood vessels revealed...
We have shown that DNA methylation of the PITX2 gene predicts risk distant recurrence in steroid hormone receptor-positive, node-negative breast cancer. Here, we present results from a multicenter study investigating whether and other candidate markers predict outcome node-positive, estrogen HER-2-negative cancer patients who received adjuvant anthracycline-based chemotherapy.Using microarray platform, analyzed regulatory regions 60 additional genes 241 specimens. Using Cox regression...
To analyze the molecular basis of gap junctional communication in mouse retina, we examined expression pattern following 13 connexin (Cx) genes: Cx26, Cx30, Cx30.3, Cx31, Cx31.1, Cx32, Cx36, Cx37, Cx40, Cx43, Cx45, Cx46, and Cx50. By using reverse transcriptase-polymerase chain reactions with primer oligonucleotides to murine genes, detected mRNAs Retinae from heterozygous mice targeted replacement most Cx45 open reading frame by a lacZ reporter gene showed promoter activity somata ganglion...
The objective of this study was to compare the pharmacokinetics and pharmacodynamics insulin aspart (IA), a rapidly acting analog, with those human soluble (regular) (HI) in animal models after s.c. i.v. dosing. Single doses IA HI were administered rats dogs at three dose levels, one level pigs; also underwent repeated dosing for 1 week. Plasma levels assessed predetermined time points dosing; plasma glucose measured pigs only. There no significant pharmacokinetic differences between single...
High-throughput next-generation shotgun sequencing of pathogenic bacteria is growing in clinical relevance, especially for chromosomal DNA-based taxonomic identification and antibiotic resistance prediction. Genetic exchange facilitated extrachromosomal DNA, e.g. plasmid-borne genes. Consequently, accurate plasmids from whole-genome (WGS) data remains one the major challenges sequencing-based precision medicine infectious diseases. Here, we assess heterogeneity four state-of-the-art tools...
Emerging antibiotic resistance is a major global health threat. The analysis of nucleic acid sequences linked to susceptibility phenotypes facilitates the study genetic determinants inform molecular diagnostics and drug development. We collected data (11,087 newly-sequenced whole genomes) culture-based profiles (10,991 out 11,087 isolates comprehensively tested against 22 antibiotics in total) clinical including 18 main species spanning time period 30 years. Species specific patterns were...
Multidrug-resistant pathogens represent one of the biggest global healthcare challenges. Molecular diagnostics can guide effective antibiotics therapy but relies on validated, predictive biomarkers. Here we present a novel, universally applicable workflow for rapid identification antimicrobial resistance (AMR) biomarkers from clinical Escherichia coli isolates and quantitatively evaluate potential to recover causal observed phenotypes. For this, metagenomic plasmid library 1,110 E. was...
Whole-genome sequencing (WGS) is gaining importance in the analysis of bacterial cultures derived from patients with infectious diseases. Existing computational tools for WGS-based identification have, however, been evaluated on previously defined data relying thereby unwarily available taxonomic information.Here, we newly sequenced 846 clinical gram-negative isolates representing multiple distinct genera and compared performance five (CLARK, Kaiju, Kraken, DIAMOND/MEGAN TUIT). To establish...
To analyze the molecular basis of gap junctional communication in mouse retina, we examined expression pattern following 13 connexin (Cx) genes: Cx26, Cx30, Cx30.3, Cx31, Cx31.1, Cx32, Cx36, Cx37, Cx40, Cx43, Cx45, Cx46, and Cx50. By using reverse transcriptase-polymerase chain reactions with primer oligonucleotides to murine genes, detected mRNAs Retinae from heterozygous mice targeted replacement most Cx45 open reading frame by a lacZ reporter gene showed promoter activity somata ganglion...
Over the last few decades, a wealth of treatment options have become available for breast cancer. To specifically direct those therapies to patients with highest need who will receive greatest benefit, biomarkers are urgently needed. Two specific needs seem be most pressing: first is prognostic markers, which would determine group may recover without adjuvant chemotherapy. Second, predictive markers treatments, such as different endocrine chemotherapies or targeted drugs, expected play major...
Supplementary Data from DNA Methylation Markers Predict Outcome in Node-Positive, Estrogen Receptor-Positive Breast Cancer with Adjuvant Anthracycline-Based Chemotherapy
<div>Abstract<p><b>Purpose:</b> We have shown that DNA methylation of the PITX2 gene predicts risk distant recurrence in steroid hormone receptor-positive, node-negative breast cancer. Here, we present results from a multicenter study investigating whether and other candidate markers predict outcome node-positive, estrogen HER-2-negative cancer patients who received adjuvant anthracycline-based chemotherapy.</p><p><b>Experimental Design:</b>...
<div>Abstract<p><b>Purpose:</b> We have shown that DNA methylation of the PITX2 gene predicts risk distant recurrence in steroid hormone receptor-positive, node-negative breast cancer. Here, we present results from a multicenter study investigating whether and other candidate markers predict outcome node-positive, estrogen HER-2-negative cancer patients who received adjuvant anthracycline-based chemotherapy.</p><p><b>Experimental Design:</b>...
Supplementary Data from DNA Methylation Markers Predict Outcome in Node-Positive, Estrogen Receptor-Positive Breast Cancer with Adjuvant Anthracycline-Based Chemotherapy