Antimicrobial peptides (AMPs) are the main components of plant innate immune system. Defensins represent most important AMP family involved in defense and non-defense functions. In this work, global RNA sequencing de novo transcriptome assembly were performed to explore diversity defensin-like (DEFL) genes wheat Triticum kiharae study their role induced resistance (IR) mediated by elicitor metabolites a non-pathogenic strain FS-94 Fusarium sambucinum . Using combination two pipelines for...

Hevein-like antimicrobial peptides (AMPs) comprise a family of plant AMPs with antifungal activity, which harbor chitin-binding site involved in interactions chitin fungal cell walls. However, the mode action hevein-like remains poorly understood. This work reports structure–function relationship WAMPs—hevein-like found wheat (Triticum kiharae Dorof. et Migush.) and later other Poaceae species. The effect WAMP homologues differing at position 34 activity peptide fragments derived from...

Cysteine-rich peptides (CRPs) play an important role in plant physiology. However, their resistance induced by biogenic elicitors remains poorly understood. Using whole-genome transcriptome sequencing and our CRP search algorithm, we analyzed the repertoire of CRPs tomato Solanum lycopersicum L. response to Fusarium oxysporum infection from F. sambucinum. We revealed 106 putative transcripts belonging different families antimicrobial (AMPs), signaling (RALFs), with non-defense functions...

Non-specific lipid-transfer proteins (nsLTPs) represent a family of plant antimicrobial peptides (AMPs) implicated in diverse physiological processes. However, their role induced resistance (IR) triggered by non-pathogenic fungal strains and metabolites is poorly understood. In this work, using RNA-seq data our AMP search pipeline, we analyzed the repertoire nsLTP genes wheat Triticum kiharae studied expression response to Fusarium oxysporum infection treatment with intracellular sambucinum...

Plants have evolved a complex multilayered defense system to counteract various invading pathogens during their life cycle. In addition silencing, considered be major molecular response against viruses, different signaling pathways activated by phytohormones trigger the expression of secondary metabolites and proteins preventing virus entry propagation. this study, we explored cucumber plants one global pathogens, green mottle mosaic (CGMMV), which causes severe symptoms on leaves fruits....

Antimicrobial peptides (AMPs) are important components of defense system in both plants and animals. They represent an ancient mechanism innate immunity providing rapid first line against pathogens. Plant AMPs classified into several families: thionins, defensins, nonspecific lipid-transfer proteins, hevein- knottin-type peptides, hairpinins macrocyclic (cyclotides). The review focuses on the thionin family. Thionins comprise a plant-specific AMP family that consists short (~5 kDA)...

Antimicrobial peptides (AMPs) constitute an essential part of the plant immune system. They are regarded as alternatives to conventional antibiotics and pesticides. In this study, we have identified γ-core motifs, which associated with antimicrobial activity, in 18 AMPs from grasses assayed their properties against nine pathogens, including yeasts affecting humans, well pathogenic bacteria fungi. All tested displayed properties. We discovered a number short AMP-derived high activity both...

Peptides play an essential role in plant development and immunity. Filipendula ulmaria, belonging to the Rosaceae family, is a medicinal which exhibits valuable pharmacological properties. F. ulmaria extracts vitro inhibit growth of variety human pathogens. The peptides defense against pathogens remains unknown. objective this study was explore repertoire antimicrobial (AMPs) defense-related signaling peptide genes expressed by response infection with Bipolaris sorokiniana using RNA-seq....

Plant extracts and microbial culture liquids contain a great number of bioactive substances, including potential biofungicides, which are effective against plant deseases.Screening cultural filtrates for ability to suppress several pathogenic fungi that damage various agricultural plants can result in discovery new compounds with broad range fungicidal activity.This work is the first report on vitro testing activity extracts, obtained by serial extractions Chenopodium album seeds hexane...

Five wild plant species belonging to different families (Chenopodium album, Plantago major, Elytrigia elongata, Filipendula ulmaria and Nigella sativa) widely spread in Russian Federation the former USSR were evaluated for their ability inhibit growth of two important human food-borne pathogens (Escherichia coli O157:H7 Listeria monocytogenes strain EGD-e) eight (Alternaria alternata, Alternaria tenuissima, Bipolaris sorokiniana, Stagonospora nodorum, Fusarium solani, oxysporum, culmorum...

There are increasing environmental risks associated with extensive use of fungicides for crop protection. Hence, the new approaches using natural plant defense mechanisms, including application antimicrobial peptides (AMPs), is great interest. Recently, we studied structural–function relationships between antifungal activity and five hevein-like AMPs from WAMP (wheat AMP) family Triticum kiharae Dorof. et Migush. We first discovered that short derived central, N-, C-terminal regions one...

Antimicrobial peptides (AMPs) constitute an important part of the plant immune system protecting plants from invading pathogens.Some antimicrobial are also active against human pathogenic microorganisms, including antibiotic-resistant strains that makes these molecules promising candidates for design next-generation drugs to treat infections.Plants represent a valuable source effective yet poorly explored peptides.An efficient pipeline highthroughput in silico detection Lycopersicon...

М.П. СЛЕЗИНА, Е