A5023404099- Amino Acid Enzymes and Metabolism
- Drug Transport and Resistance Mechanisms
- Genetics and Neurodevelopmental Disorders
- Pharmacological Effects and Toxicity Studies
- Genomic variations and chromosomal abnormalities
- Epigenetics and DNA Methylation
- Cannabis and Cannabinoid Research
- DNA and Nucleic Acid Chemistry
- Molecular Sensors and Ion Detection
- HIV/AIDS drug development and treatment
- Biochemical effects in animals
- Metabolism and Genetic Disorders
- Stress Responses and Cortisol
- Hemoglobinopathies and Related Disorders
- Pharmacogenetics and Drug Metabolism
- RNA and protein synthesis mechanisms
- Genomics and Rare Diseases
- Estrogen and related hormone effects
- Menopause: Health Impacts and Treatments
University of California, San Francisco
2020-2024
Hunter College
2016
City University of New York
2016
17β-estradiol (E2) rapidly, within minutes, activates behaviors and cognition by binding to membrane estrogen receptors, activating cell signaling cascades increasing dendritic spines. In female rodents, E2 enhances spatial memory 2-4 hours, spine density is increased in the CA1 area of hippocampus 30-60 minutes. Although chronic gonadal hormone treatments male rats alter spines/spine synapses acute effects occur hippocampal slices, acute, vivo administration males are unknown. Therefore, we...
Heterozygous variants in SLC6A1, encoding the GAT-1 GABA transporter, are associated with seizures, developmental delay, and autism. The majority of affected individuals carry missense variants, many which recurrent germline de novo mutations, raising possibility gain-of-function or dominant-negative effects. To understand functional consequences, we performed an vitro uptake assay for 213 unique including 24 control variants. De consistently resulted a decrease uptake, keeping...
The human solute carrier 22A (SLC22A) family consists of 23 members, representing one the largest families in SLC superfamily. Despite their pharmacological and physiological importance absorption disposition a range solutes, eight SLC22A members remain classified as orphans. In this study, we used multifaceted approach to identify ligands orphan SLC22A15. Ligands SLC22A15 were proposed based on phylogenetic analysis comparative modeling. putative then confirmed by metabolomic screening...
Membrane transporters play a fundamental role in the tissue distribution of endogenous compounds and xenobiotics are major determinants efficacy side effects profiles. Polymorphisms within these drug result inter-individual variation response, with some patients not responding to recommended dosage whereas others experience catastrophic effects. For example, variants hepatic Human organic cation transporter OCT1 (SLC22A1) can change cations many prescription levels. To understand how...
The ATP-binding cassette transporter P-glycoprotein (P-gp) limits the oral bioavailability of many drugs. Although P-gp has been well studied in humans and mice, little is known about substrate specificities its species orthologs. To address this, we performed vitro analysis function using HEK293 cells stably expressing human, ovine, porcine, canine, feline P-gp. We also employed a human physiologically based pharmacokinetic (PBPK) model to assess variations digoxin exposure resulting from...
Abstract Heterozygous variants in the GAT-1 GABA transporter encoded by SLC6A1 are associated with seizures, developmental delay, and autism. The majority of affected individuals carry missense variants, many which recurrent germline de novo mutations, raising possibility gain-of-function effects. To understand functional consequences, we performed an vitro uptake assay for 213 unique including 24 control variants. De consistently resulted a decrease uptake, keeping haploinsufficiency...
ABSTRACT The human L-type amino acid transporter 1 (LAT1; SLC7A5) is a membrane of acids, thyroid hormones, and drugs such as the Parkinson’s disease drug L-Dopa. LAT1 found in blood-brain-barrier (BBB), testis, bone marrow, placenta, its dysregulation has been associated with various neurological diseases autism epilepsy well cancer. In this study, we combine metainference molecular dynamics (MD) simulations, docking, experimental testing, to characterize LAT1-inhibitor interactions. We...