A5067978228- Advanced NMR Techniques and Applications
- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects and Toxicity Studies
- Enzyme Structure and Function
- Gastroesophageal reflux and treatments
- Barrier Structure and Function Studies
- Metal-Organic Frameworks: Synthesis and Applications
- Pharmaceutical studies and practices
- Amino Acid Enzymes and Metabolism
- Medical Imaging Techniques and Applications
- Central Venous Catheters and Hemodialysis
- Intensive Care Unit Cognitive Disorders
- Antibiotics Pharmacokinetics and Efficacy
- Magnetism in coordination complexes
- Pharmacogenetics and Drug Metabolism
- Gastrointestinal motility and disorders
- Lanthanide and Transition Metal Complexes
- HIV/AIDS drug development and treatment
University of California, San Francisco
2021-2024
University of California System
2022
Purpose The combined hyperpolarized (HP) 13 C pyruvate and urea MRI has provided a simultaneous assessment of glycolytic metabolism tissue perfusion for improved cancer diagnosis therapeutic evaluation in preclinical studies. This work aims to translate this dual‐probe HP imaging technique clinical research. Methods A co‐polarization system was developed where [1‐ C]pyruvic acid (PA) [ C, 15 N 2 ]urea water solution were homogeneously mixed polarized on 5T SPINlab system. Physical chemical...
This study aimed to develop and demonstrate the in vivo feasibility of a 3D stack-of-spiral balanced steady-state free precession(3D-bSSFP) urea sequence, interleaved with metabolite-specific gradient echo (GRE) sequence for pyruvate metabolic products, improving SNR spatial resolution first hyperpolarized 13 C-MRI human injection co-hyperpolarized [1-13 C]pyruvate [13 C,15 N2 ]urea.A bSSFP imaging was designed using urea-specific excitation pulse, optimized TR, readouts. Simulations phantom...
Solid tumors such as prostate cancer (PCa) commonly develop an acidic microenvironment with pH 6.5-7.2, owing to heterogeneous perfusion, high metabolic activity, and rapid cell proliferation. In preclinical models, disease progression is associated a decrease in tumor extracellular pH, suggesting that imaging may reflect biomarker detect aggressive high-risk disease. Therefore, we developed hyperpolarized carbon-13 MRI method image the (pHe) prepared it for clinical translation detection...
Abstract The Blood-Brain Barrier (BBB) is a crucial, selective barrier that regulates the entry of molecules including nutrients, environmental toxins, and therapeutic medications into brain. This function relies heavily on brain endothelial cell proteins, particularly transporters tight junction proteins. BBB continues to develop postnatally, adapting its across different developmental phases, alters with aging disease. Here we present global proteomics analysis focused ontogeny proteins in...
Introduction The healthy heart has remarkable metabolic flexibility that permits rapid switching between mitochondrial glucose oxidation and fatty acid to generate ATP. Loss of been implicated in the genesis contractile dysfunction seen cardiomyopathy. Metabolic imaged experimental models, using hyperpolarized (HP) [2‐ 13 C]pyruvate MRI, which enables interrogation metabolites reflect tricarboxylic (TCA) cycle flux cardiac myocytes. This study aimed develop methods, demonstrate feasibility...
The ATP-binding cassette transporter P-glycoprotein (P-gp) limits the oral bioavailability of many drugs. Although P-gp has been well studied in humans and mice, little is known about substrate specificities its species orthologs. To address this, we performed vitro analysis function using HEK293 cells stably expressing human, ovine, porcine, canine, feline P-gp. We also employed a human physiologically based pharmacokinetic (PBPK) model to assess variations digoxin exposure resulting from...
Hyperpolarized [1-13C]alpha-ketoglutarate (aKG) is a metabolic probe that can provide direct access to the pathway for glutamate production or 2-HG in altered alpha-ketoglutarate metabolism via IDH mutations. With its potential serve as biomarker of IDH1 mutational status glioma and measure aKG normal healthy subjects, we developed new chemistry preparation standard operating procedure (SOP) on-site hyperpolarized GMP characterized investigate feasibility this clinical setting. Following...
Motivation: Isocitrate dehydrogenase (IDH) mutational status is crucial for accurate diagnosis and prognosis of malignant gliomas. However, the current clinical assessment IDH mutation requires an invasive brain biopsy pathological testing. Goal(s): We aimed to perform first in-human MR studies using hyperpolarized [1-13C]alpha-ketoglutarate as a new probe via cancer metabolic reprogramming, along with cerebral bioenergetics. Approach: acquired 13C MRS data from healthy volunteers (N=6)...
Abstract Summary Introduction The normal heart has remarkable metabolic flexibility that permits rapid switching between mitochondrial glucose oxidation and fatty acid (FA) to generate ATP. Loss of been implicated in the genesis contractile dysfunction seen cardiomyopathy. Metabolic imaged experimental models, using hyperpolarized (HP) [2- 13 C]pyruvate MRI, which enables interrogation metabolites reflect tricarboxylic (TCA) cycle flux cardiac myocytes. This study aimed develop methods,...
Constipation is frequent in critically ill adults receiving opioids. Naloxegol (N), a peripherally acting mu-receptor antagonist (PAMORA), may reduce constipation. The objective of this trial was to evaluate the efficacy and safety N prevent constipation ICU Methods Patients. In single-center, double-blind, randomized trial, admitted medical IV opioids (≥100 mcg fentanyl/day), not having any 17 exclusion criteria, were (25 mg) or placebo (P) daily receive (25mg) docusate 100 mg twice until...
Background: Recent shortages of intravenous (IV) fluids have resulted in healthcare systems converting administration many medications from IV piggyback (IVPB) to push (IVP). Administering via IVP presents numerous advantages; however, site reactions such as phlebitis and infiltration may occur. Objective: The objective this analysis is evaluate the infusion safety ertapenem given peripheral compared IVPB adult patients. Methods: This was an institutional review board-approved,...