Endophytic fungi play a vital role in protecting and promoting the growth of their host plants. The diversity fungal endophytes has been documented across different plant species varies depending on factors such as host, ecological conditions, health status plant. We isolated endophytic from Prunus yedoensis trees with decay rates. A total 31 were decayed trees, while 33 obtained healthy trees. number exhibiting antifungal activities against wood was higher 10 showing activity compared to...

A variety of commercial instruments are available for sampling and quantifying microscopic airborne organisms from the environment. Although most samplers highly sensitive, they also expensive, costing thousands dollars per unit, a price that is out reach many researchers, especially those looking to design experiments with replication. While at options monitor pine stands presence Fusarium circinatum, causal agent pitch canker disease, on multiple sites several units site, we developed...

Numerous cankers on small branches showing dieback were observed live oak (Quercus virginiana) trees in September 2010 Marion County, FL. Approximately 24 12-year-old landscape planted a farm displayed symptoms. Samples collected from six of the symptomatic and returned to laboratory for processing. Isolations made canker margins after surface sterilization samples 2.5% sodium hypochlorite by plating potato dextrose agar (PDA). A suspect Botryosphaeriaceae sp. (based colony morphology) was...

Laurel wilt is a fungal vascular disease of redbay (Persea borbonia (L.) Spreng) and other plants in the family Lauraceae southeastern United States (1). The caused by Raffaelea lauricola T. C. Harr., Fraedrich & Aghayeva, which vectored exotic ambrosia beetle (Xyleborus glabratus Eichhoff) (2). Pondspice (Litsea aestivalis Fern.) an obligate wetland shrub listed as endangered Florida Maryland threatened Georgia (4). On 29 August 2008, 369 430 (85%) pondspice trees observed at St. Marks...

Essential oils have insecticidal activity against several insects and are composed of numerous compounds. This study investigated the effects Thuja occidentalis L. (Cupressaceae) essential oil, terpinyl acetate, bornyl acetate peach aphid [Myzus persicae (Sulzer)]. The effect on three Cupressaceae plants was highest in T. occidentalis. oil extracted by steam distillation, optimum conditions were found. GC-MS profiling components from leaves identified 16 volatile main α-thujone (16.58%),...

Ganoderma (Ganodermataceae, Basidiomycota) species are medicinally and economically important wood-decaying fungi occurring throughout the world. Since 2022, unrecorded have been collected in Jinju Busan, Korea. The hosts of fungal were Platanus Prunus species. Based on morphological characteristics molecular phylogenetic analysis, this was identified as G. gibbosum. Description, macroscopic microscopic photographs, a phylogeny based ITS sequences provided here.

A key role that fungal endophytes play in interacting with their host plant can be defined by the fact they promote growth of plants and enhance tolerance against pathogens using bioactive compounds produce. Several studies utilizing endophytic fungi as a source biological control were conducted, representative example includes Aureobasidium protae from common wheat (Triticum aestivum), which inhibited mycelial Fusarium graminearum culmorum, causal agents head blight disease. Our previous...

Pollutants can exist in the soil for a long time and alter bacterial community. Using lubricants to prevent wear of chainsaw blades is necessary thinning activities wood harvesting. We investigated influences contamination with on communities. Bio-oil, mineral oil, recycled oil were scattered each treatment investigate variations structure during treated periods using Illumina MiSeq sequencing platform. The results obtained 5943 ASVs, 5112 6136 ASVs after at one month, six months, twelve...

A canker disease of Florida torreya (Torreya taxifolia) has been implicated in the decline this critically endangered species its native range northern and southeastern Georgia. In surveys eight sites, cankers were present on all dead trees 71 to 100% living trees, suggesting that a fungal pathogen might be causal agent. To identify agent, nuclear ribosomal internal transcribed spacer region (ITS rDNA) sequences determined for 115 fungi isolated from 46 symptomatic sampled at three sites...

The availability of adapted germplasm capable regenerating shoots from callus may affect the speed with which tissue culture‐mediated genetic improvements can be brought to commercial usage. To assess shoot regeneration capability in Beta vulgaris L., 17 sources including sugarbeet, table beet, fodder beet and leaf were screened for callus‐forming ability regeneration. Petiole blade explants vitro cultures grown 6‐benzyladenine (BA) as only growth regulator challenged initiate on a...

Silk bay (Persea humilis Nash) is a member of the Lauraceae precinctive to scrub forests central and southern Florida sister species primary laurel wilt host, redbay (P. borbonia (L.) Spreng), which generally not found in these ecosystems. In November 2011, observations silk mortality near Lake Placid Highlands County, FL, were reported Forest Service agents. A subsequent visit roadside homeowners' properties area revealed many dead dying silkbays with characteristic symptoms, including...

Since 2006 there has been a decline in Colorado blue spruce (CBS; Picea pungens) planted as landscape trees and for Christmas tree production throughout the Lower Peninsula of Michigan. This is characterized by slow loss needles lower portion starting at branch tips, followed entire dieback, which progresses upward over several years. dieback linked to shallow cankers visible phloem when bark layer removed. Isolates fungal genus Diaporthe have consistently isolated from lesion margins on...

Vanderbylia fraxinea (Bull.) D.A. Reid, 1973 is an important wood-inhabiting fungus that plays a significant role in nutrient recycling most forest ecosystems. In this study, the complete mitochondrial genome of V. was characterized through de novo assembly using Illumina sequencing data and annotation. The circular molecule 115,473 bp with GC content 28.66%. It comprises total 62 genes. Among these, 36 are protein-coding genes including 21 free-standing open reading frames (ORFs), 24...

Pluripotent stem cells (PSCs) have the ability of self-renewal that can retain characteristics mother cell, and pluripotency differentiate into several body types. PSCs typically include embryonic (ESCs) derived from inner cell mass preimplantation embryo, epiblast (EpiSCs) postimplantation embryo. Although are able to be used by differentiation endothelial as a potential treatment for vascular diseases, human ESCs induced (iPSCs) followed ethical safety issues. Pigs anatomically...

A routine survey to establish an inventory of fungal pathogens occurring on Quercus spp. in South Korea was conducted the autumn 2021 and 2022. Symptoms, including leaf spot, were frequently observed variabilis leaves a natural forest located Sejong. fungus with unique morphological characteristics, pycnothyria, found be major colonizer infected Q. variabilis. Based characteristics fungus, it identified as member Tubakia. Sequence comparisons LSU, ITS, TEF-1α gene regions revealed that...

Machilus thunbergii Siebold & Zucc., known as Japanese bay tree, is an evergreen tree distributed widely in East Asia, including South Korea, where the species of ecological importance. provides habitat for wildlife and a common urban tree. In September 2022, anthracnose symptoms on leaves were observed Jeju (33°26'02.4"N, 126°19'48.8"E) Tongyeong (34°49'27.1"N, 128°24'01.8"E) Korea. Disease incidence each affected naturally growing forest area covering approximately 0.5 ha was ~ 70 % study...

Lubricants are applied onto chainsaw blades to achieve their optimum cutting performance; however, during logging or timber operations, lubricants may penetrate the forest soil. The persistent organic pollutants in cause environmental damage, and different types of vary terms impact. Hence, selecting appropriate for operations is important sustainable management. In this study, effects three lubricant types—biodegradable oil (bio-oil), petroleum-based bar-and-chain (mineral oil), recycled...

Porcine embryonic stem cells (pESCs) would provide potentials for agricultural- and biotechnological-related applications. However, authentic pESCs have not been established yet because standards porcine cell-specific markers culture conditions are clear. Therefore, the present study reports attempts to derive pluripotent epiblast either from in vitro or vivo derived embryos. Nine cell lines (seven Berkshire two Duroc) could only be isolated day 9- 9.5-old early conceptuses. Pluripotency...