A5005489019- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- Microplastics and Plastic Pollution
- Animal Genetics and Reproduction
- Mesenchymal stem cell research
- Effects and risks of endocrine disrupting chemicals
- Diverse Topics in Contemporary Research
- Antimicrobial agents and applications
- GABA and Rice Research
- Toxin Mechanisms and Immunotoxins
- Protein Interaction Studies and Fluorescence Analysis
- Recycled Aggregate Concrete Performance
- Animal testing and alternatives
- 3D Printing in Biomedical Research
- Histone Deacetylase Inhibitors Research
- Psychosocial Factors Impacting Youth
- Genetic Mapping and Diversity in Plants and Animals
- Congenital heart defects research
- Child and Adolescent Health
- Biochemical and Molecular Research
- Environmental Chemistry and Analysis
- Diverse Approaches in Healthcare and Education Studies
- Mechanisms of cancer metastasis
Gyeongsangnam-do Agricultural Research and Extension Services
2015-2023
Gyeongsang National University
2014-2023
Korea Institute of Toxicology
2020-2022
University of Ulsan
2019-2021
Asan Medical Center
2019-2021
Ulsan College
2019-2021
Chungbuk National University
2020
Korea Environment Institute
2020
The establishment of porcine epiblast stem cells (pEpiSCs) and induced pluripotent (piPSCs) derived from diametrical derivations is great importance in developing biomedical models. However, pEpiSCs piPSCs have been technically much harder to culture than mouse embryonic cells, showing problematic properties such as spontaneous differentiation apoptosis after cryopreservation. Therefore, we demonstrated that Y-27632 a Rho-associated coiled-coil containing kinase inhibitor could prevent...
Endothelial cells (ECs), lining blood vessels’ lumen, play an essential role in regulating vascular functions. As multifunctional components of structures, pluripotent stem (PSCs) are the promising source for potential therapeutic applications various diseases. Our laboratory has previously established approach differentiating porcine epiblast (pEpiSCs) into ECs, representing alternative and potentially superior cell source. However, condition pEpiSCs-derived ECs growth yet to be determined,...
Conventionally, immunotoxins have been produced as a single polypeptide from fused genes of an antibody fragment and toxin. In this study, we adopted unique approach chemical conjugation toxin protein fragment. The two were separately expressed in Escherichia coli purified to high levels purity. proteins conjugated using linker. advantage is its ability overcome the problem low recombinant immunotoxin production observed some immunotoxins. Another that various combinations can be prepared...
Inhalation exposure to polyhexamethylene guanidine phosphate (PHMG-P), one of the primary biocides used in humidifier disinfectants, caused a fatal pulmonary disease Korea. Pregnant women were also exposed PHMG-P, and subsequent studies showed that PHMG-P inhalation during pregnancy adversely affects their health embryo-fetal development. However, postnatal developmental effects after birth on prenatally PHMG-P-exposed offspring have not yet been investigated. Therefore, this study, we aimed...
The development of embryos reconstructed by nuclear transfer (NT) is dependent upon numerous factors. Central to the quality and recipient cytoplast. Typically oocytes Corresponding author: Joon-Hee Lee Tel: +82-55-772-1886 Fax: +82-55-772-1889 E-mail: sbxjh@gnu.ac.kr 168 ... Journal Agriculture & Life Science 47(6) at either AI/TI or MII are potential candidates used as cytoplasts for NT, because they contain active MPF, which causes NEBD PCC in transferred nucleus may be essential...
Activation-induced cytidine deaminase (AID) is the only enzyme that has been suggested as a putative DNA demethylase in mammals. However, very little known about AID function of bovine differentiated cells toward pluripotent state. To investigate effect on demethylation, complementary DNAs were transfected into cells, which mostly methylated promoter regions pluripotency genes. As result, AID-transfected started to transform colonies at day 19 transfection. The derived from showed positive...
In-Won Lee, Hyeon-Geun Dae-Ky Moon, Yeon-Ji Bo-Gyeong Seo, Sang-Ki Baek, Tae-Suk Kim, Cheol Hwangbo and Joon-Hee Lee. Journal of Animal Reproduction Biotechnology 2023;38:109-20. https://doi.org/10.12750/JARB.38.3.109
Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated can convert into undifferentiated or pluripotent state. In the SCNT experiment, reprogramming is induced by exposure of introduced donor nuclei to recipient cytoplasm matured oocytes. However, because efficiency still remains low, combination technique with ex-ovo method may improve normal development embryos. Here we hypothesized treatment somatic cells extracts prepared from germinal vesicle (GV) stage...
Bis-diamine was developed as amebicidal and male contraceptive agents; however, it is also reported to induce characteristic congenital heart defects especially in the cardiac conotruncal area of rats. Because its defects, bis-diamine-induced animal models can be used for studying defects. However, comprehensive toxicological information regarding this model not available.In study, we investigated characterized an A single dose 200-mg bis-diamine administered by oral gavage pregnant rats on...
Pluripotent stem cells (PSCs) have the ability of self-renewal that can retain characteristics mother cell, and pluripotency differentiate into several body types. PSCs typically include embryonic (ESCs) derived from inner cell mass preimplantation embryo, epiblast (EpiSCs) postimplantation embryo. Although are able to be used by differentiation endothelial as a potential treatment for vascular diseases, human ESCs induced (iPSCs) followed ethical safety issues. Pigs anatomically...
Sang-Ki Baek, In-Won Lee, Yeon-Ji Bo-Gyeong Seo, Jung-Woo Choi, Tae-Suk Kim, Cheol Hwangbo and Joon-Hee Lee. Journal of Animal Reproduction Biotechnology 2023;38:275-90. https://doi.org/10.12750/JARB.38.4.275
Conventional human pluripotent stem cell (hPSC) cultures require high concentrations of expensive fibroblast growth factor 2 (hFGF-2) for hPSC self-renewal and pluripotency in defined media long-term culture. The thermal instability the hFGF-2 mandates change every day, which makes culture costly cumbersome. Human DJ-1 (hDJ-1) can bind to stimulate FGF receptor-1. In this study, first time, we have replaced with hDJ-1 essential eight maintained embryonic cells (hESCs), H9, at feeder-free...
Porcine embryonic stem cells (pESCs) would provide potentials for agricultural- and biotechnological-related applications. However, authentic pESCs have not been established yet because standards porcine cell-specific markers culture conditions are clear. Therefore, the present study reports attempts to derive pluripotent epiblast either from in vitro or vivo derived embryos. Nine cell lines (seven Berkshire two Duroc) could only be isolated day 9- 9.5-old early conceptuses. Pluripotency...
‘게임 이용동기’, 효능감’, 규범성’ 및 인식’이 종속변수인 ‘게임선용’에 미치는 영향을 다층모형으로 분석했다. 게임 이용행태 4개 변수와 시간수준인 측정시점으로 구성한 다층모형 분석결과, 측정시점 분산이 52∼98%, 이용행태가 2∼48%로 각각 나타나 측정시점이 개인수준보다 평균 세배 정도 많은 주는 것으로 나타났다. 개인수준인 중에서는 ‘게임효능감’, ‘게임선용’의 6개 하부 요인에 정적 영향력을 보였다. 이용동기’의 ‘몰두’ 요인은 ‘여가 선용’에는 부적, ‘활력 경험’, ‘생활경험 확장’, ‘게임몰입’ ‘통제력 경험’에는 미쳤다. ‘사회적 동기’는 선용’, ‘자긍심 ‘게임몰입’에 확장’에 ‘성취감’은 경험’ 모두 인구통계학적 변수의 ‘성’은 남성에 대해 확장’ 경험’이 정적, ‘연령’은 부적 이용자 연구에서 다층모형분석을 바탕으로 한 시계열 연구의 중요성을 설명하고, 개인수준의 대한 영향의 이론적 함의를 논의했다.
Human stem-cell factor (hSCF) stimulates the survival, proliferation, and differentiation of hematopoietic cells by binding to c-Kit receptor. Various applications hSCF require efficient reliable production hSCF. exists in three forms: as two membrane-spanning proteins hSCF248 hSCF229 truncated soluble N-terminal protein hSCF164. hSCF164 is known be insoluble when expressed Escherichia coli cytoplasm, requiring a complex refolding procedure. The activity has never been studied. Here, we...
본 연구는 순환굵은골재 콘크리트의 활용성 증대 및 규준 정립에 관한 연구의 일환으로 치환율에 따른 고강도 철근콘크리트 보의 휨 특성을 검토하고자 한다. 실험은 콘크리트 설계강도 40MPa, 50MPa, 60MPa 치환율 0%, 30%, 50%, 100%를 변수로 총 12개의 실험체를 제작하여 최종 파괴 시까지 변위제어 방식에 의해 2점 가력하였다. 실험결과 천연골재를 사용한 실험체와 비교시 균열발생 파괴양상의 경우, 순환굵은골재를 실험체는 천연골재 보 실험체에 비해 균열이 비교적 압축측까지 진전하는 보였으나 전반적으로 균열양상은 유사하게 나타났으며, KCI 설계기준식에 의한 계산값 실험결과의 비교결과 관계없이 1.08~1.29로 기준값을 상회하는 것으로 나타나 부재설계에 적용 가능할 판단된다. 따라서 증대를 위해 추가적인 실험을 통하여 순환굵은골재의 구조적 기준정립이 필요할 Recently, natural aggregate was adequate to supply the...
The cloning efficiency is extremely low despite successful somatic cell nuclear transfer (SCNT) method producing cloned animals in several mammals. In general, faulty epigenetic modifications underlying the incomplete reprogramming of donor nuclei after SCNT mainly results efficiency. process involves modifications, such as DNA demethylation and histone acetylation, which may be an important factor enhancing Recently, deacetylase inhibitors (HDACi), trichosatin A (TSA) m-carboxycinnamic acid...