A5079948580- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Epilepsy research and treatment
- Neurobiology and Insect Physiology Research
- Neurotransmitter Receptor Influence on Behavior
- Ion Transport and Channel Regulation
- Lepidoptera: Biology and Taxonomy
- Diet and metabolism studies
- Neurological disorders and treatments
- Insect Utilization and Effects
- Photoreceptor and optogenetics research
- Memory and Neural Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Mitochondrial Function and Pathology
- Cholinesterase and Neurodegenerative Diseases
- Insect Resistance and Genetics
- Silkworms and Sericulture Research
- Bone health and treatments
- Neuroendocrine regulation and behavior
- Medical Imaging Techniques and Applications
- Genetic Neurodegenerative Diseases
- Zebrafish Biomedical Research Applications
- Chemical synthesis and alkaloids
Aarhus University
2010-2023
University of Copenhagen
1983-2008
University of Oxford
1988-1989
University of Massachusetts Amherst
1968
1. The membrane properties of substantia nigra pars compacta (SNc) neurones were recorded in guinea‐pig vitro brain slices. 2. In the presence tetrodotoxin (TTX) a Ca(2+)‐dependent slow oscillatory potential (SOP) was generated. Application 0.5‐20 microM nifedipine abolished both spontaneous and evoked SOPs. A tetraethylammonium chloride (TEA)‐promoted high‐threshold Ca2+ spike (HTS) little affected by nifedipine. On other hand, omega‐conotoxin applied either locally or via perfusion medium...
The effect of a brief train electric stimuli in the dorsolateral funiculus on intrinsic response properties turtle motoneurones was investigated transverse sections spinal cord vitro. Even when glutamatergic, GABAergic and glycinergic ionotropic synaptic transmission blocked by antagonists AMPA, NMDA, glycine GABA receptors, (DLF) stimulation induced facilitation plateau potentials during current clamp underlying inward voltage clamp. This lasted more than 10 s. potential DLF absent presence...
ABSTRACT From two lines of evidence, we conclude that the potassium transport gives rise directly to midgut potential, i.e. active mechanism is electrogenic. First, diffusion potentials neither potassium, sodium, magnesium, calcium, nor chloride could give large potential if values for tissue concentrations are accepted their respective activities in epithelium. Secondly, no externally added cation other than required sustain either or short circuit current, specific anion required, and...
1. Although the addition of 32 mM/l. choline chloride to standard bathing solutions depresses short-circuit current isolated midgut, replacement added by either sodium or lithium has no further affect on current. No net flux is measureable atomic absorption spectrometry. Therefore neither nor actively transported across midgut. 2. Rubidium able substitute fully for potassium in maintaining a The fluxes rubidium and are approximately same ratio as their concentrations blood-side midgut unable...
The Na(+)/K(+)-ATPases maintain Na(+) and K(+) electrochemical gradients across the plasma membrane, a prerequisite for electrical excitability secondary transport in neurons. Autosomal dominant mutations human ATP1A3 gene encoding neuron-specific Na(+)/K(+)-ATPase α3 isoform cause different neurological diseases, including rapid-onset dystonia-parkinsonism (RDP) alternating hemiplegia of childhood (AHC) with overlapping symptoms, hemiplegia, dystonia, ataxia, hyperactivity, epileptic...
The regenerative properties of CA1 pyramidal neurons were studied through differential polarization with external electrical fields. Recordings obtained from somata and apical dendrites in the presence 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), DL-2-amino-5-phosphonovaleric acid (APV), bicuculline. S+ fields hyperpolarized distal depolarized rest cell, whereas S÷ reversed polarization. During intradendritic recordings, evoked either fast spikes or compound spiking. threshold response...
The ketogenic diet (KD), a high-fat, carbohydrate-restricted diet, is used as an alternative treatment for drug-resistant epileptic patients. Evidence suggests that compromised glucose metabolism has significant role in the anticonvulsant action of KD; however, it unclear what part important. present study investigates how selective alterations glycolysis and oxidative phosphorylation influence epileptiform activity induced by blocking K + currents GABA A NMDA receptors hippocampal slice...
The mechanisms behind the therapeutic effects of electrical stimulation brain in epilepsy and other disorders are poorly understood. Previous studies vitro have shown that uniform electric fields can suppress epileptiform activity through a direct polarizing effect on neuronal membranes. Such an depends continuous DC with unbalanced charge. Here we describe suppressive brief (10 ms) field stimulus-evoked rat hippocampal slices exposed to Cs(+) (3.5 mM). This was independent polarity,...
ABSTRACT Amino acid accumulation within brush-border membrane vesicles (BBMV) from the larval midgut of Lepidoptera is driven by a K+ gradient. However, it can also be Na+ gradient, although with reduced efficiency. To examine possibility that sodium and potassium ions are handled same amino transporter, glycine uptake into BBMV Philosamia cynthia Drury was measured in presence pH gradient transmembrane electrical potential difference, i.e. simulated ‘physiological’ conditions. The kinetics...
Abstract Evidence is accumulating that a soluble, secretory form of acetylcholinesterase may have novel, non–cholinergic functions in certain brain regions, such as the substantia nigra. In this study, application human recombinant (rhAChE) to pars compacta neurons rostral nigra resulted sustained hyperpolarization was not only mimicked by N‐methyl–D–aSpartate (NMDA) but also blocked NMDA receptor antagonists MK801 and 2–amino–5phosphonopentanoic acid. Neither rhAChE–nor NMDA–induced seen...
Slow, pacemaker-like firing is due to intrinsic membrane properties in substantia nigra compacta (SNc) neurons vitro. How these interact with afferent synaptic inputs not fully understood. In this study, intracellular recordings from SNc brain slices showed that spontaneous action potentials (APs) were attenuated when generated lower than normal threshold. Such APs blocked by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and could be related non-N-methyl-D-aspartate (NMDA) receptor-mediated...
Abstract Reactive glial cells, for example, from patients with temporal lope epilepsy have a reduced density of inward rectifying K + (Kir) channels and thus buffering capacity. Evidence is accumulating that this downregulation Kir could be implicated in epileptogenesis. In rat hippocampal brain slices, prolonged exposure to the nonselective channel antagonist, Cs (5 mM), gives rise an epileptiform field potential (Cs‐FP) area CA1 composed initial positive (interictal‐like) phase followed by...